Stable germline transgenesis using theMinosTc1/marinerelement in the sea urchin,Lytechinus pictus DOI Creative Commons
Elliot W. Jackson, Emilio Romero, Svenja Kling

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 29, 2024

Abstract Stable transgenesis is a transformative tool in model organism biology. While the sea urchin one of oldest animal models cell and developmental biology, it has relied on transient manipulations wild animals, lacked strategy for stable transgenesis. Here we build recent progress to develop more genetically tractable species, Lytechinus pictus , establish robust transgene integration method. Three commonly used transposons ( Minos, Tol2, piggyBac ) were tested non-autonomous transposition, using plasmids containing polyubiquitin promoter upstream H2B-mCerulean nuclear marker. Minos was only transposable element that resulted significant expression past metamorphosis. F 0 animals raised sexual maturity spawned determine germline integration, inheritance frequency, characterize patterns 1 progeny. The results demonstrated transmission through germline, first example transgenic urchin, indeed any echinoderm. This milestone paves way generation diverse resources will dramatically enhance utility, reproducibility, efficiency research. Significance Statement Transgenic tools are essential effective utilization models. Despite being an established not previously benefited from technology. study reports opens new avenues this organism.

Язык: Английский

Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin, Lytechinus pictus DOI Creative Commons
Elliot W. Jackson, Emilio Romero, Svenja Kling

и другие.

Development, Год журнала: 2024, Номер 151(20)

Опубликована: Авг. 19, 2024

ABSTRACT Stable transgenesis is a transformative tool in model organism biology. Although the sea urchin one of oldest animal models cell and developmental biology, studies this have largely relied on transient manipulation wild animals, without strategy for stable transgenesis. Here, we build recent progress to develop more genetically tractable species, Lytechinus pictus, establish robust transgene integration method. Three commonly used transposons (Minos, Tol2 piggyBac) were tested non-autonomous transposition, using plasmids containing polyubiquitin promoter upstream H2B-mCerulean nuclear marker. Minos was only transposable element that resulted significant expression beyond metamorphosis. F0 animals raised sexual maturity, spawned determine germline inheritance frequency, characterize patterns F1 progeny. The results demonstrate transmission through germline, first example transgenic and, indeed, any echinoderm. This milestone paves way generation diverse resources will dramatically enhance utility, reproducibility efficiency research.

Язык: Английский

Процитировано

6
Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors DOI Creative Commons

Malcolm Arnott,

Nina Faye Sampilo,

Jia L. Song

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Апрель 24, 2024

In early embryonic development, the cross-regulation of transcription factors and signaling pathways are critical in mediating developmental physiological processes. Additionally, many studies have shown importance post-transcriptional regulation network components mediated by microRNAs (miRNAs); however, how miRNAs transcriptionally regulated is poorly understood. fine-tuners biological processes their dysregulation leads to a variety diseases defects. Previously, we that dynamically expressed throughout sea urchin suggesting likely be under transcriptional regulation. Here, used pharmacological inhibitors, genetic constructs, loss-of-function reagents assess impact key (Wnt, Nodal, MAPK, Sonic Hedgehog, Delta/Notch, VEGF, BMP) (Alx1, Ets1/2, Tbr) on transcript levels evolutionarily conserved miR-1, miR-31, miR-92 miR-124; invertebrate-specific miR-71; echinoderm-specific miR-2002, miR-2007, miR-2012. We also computational methods identify potential factor binding sites these miRNAs. Lists motifs for (TFs) were acquired from MEME-Suite Motif Database as inputs algorithm FIMO (Find Individual Occurrences), which detects short nucleotide within larger sequences. Based experimental data miRNA expression conjunction with bioinformatic predictions, propose Tbr, Alx1, Ets1 regulate SpmiR-1, SpmiR-31, SpmiR-71, respectively. additionally observed significant effects result perturbations Wnt, Hedgehog pathways, while no change or BMP pathways. Overall, this study provides insights into contribute our overall understanding

Язык: Английский

Процитировано

2
microRNA-1 Regulates Metabolic Flexibility in Skeletal Muscle via Pyruvate Metabolism DOI Creative Commons
Ahmed Ismaeel,

Bailey D. Peck,

McLane M. Montgomery

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 10, 2024

Abstract MicroRNA-1 (miR-1) is the most abundant miRNA in adult skeletal muscle. To determine function of miR-1 muscle, we generated an inducible, muscle-specific knockout (KO) mouse. Integration RNA-sequencing (RNA-seq) data from KO muscle with Argonaute 2 enhanced crosslinking and immunoprecipitation sequencing (AGO2 eCLIP-seq) human identified target genes involved glycolysis pyruvate metabolism. The loss induced cancer-like metabolic reprogramming, as shown by higher kinase isozyme M2 (PKM2) protein levels, which promoted glycolysis. Comprehensive bioenergetic phenotyping combined proteomics metabolomics further demonstrated that inflexibility a result oxidation resistance. While genetic reduced endurance exercise performance mice C. elegans, physiological down-regulation expression response to hypertrophic stimulus both humans causes similar reprogramming supports cell growth. Taken together, these identify novel post-translational mechanism metabolism regulation mediated miR-1.

Язык: Английский

Процитировано

1
In silico identification and characterization of microRNAs from rotifers, cladocerans, and copepods DOI
Min‐Sub Kim, Zhou Yang, Jae‐Seong Lee

и другие.

Marine Pollution Bulletin, Год журнала: 2024, Номер 209, С. 117098 - 117098

Опубликована: Окт. 22, 2024

Язык: Английский

Процитировано

1
Stable germline transgenesis using theMinosTc1/marinerelement in the sea urchin,Lytechinus pictus DOI Creative Commons
Elliot W. Jackson, Emilio Romero, Svenja Kling

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 29, 2024

Abstract Stable transgenesis is a transformative tool in model organism biology. While the sea urchin one of oldest animal models cell and developmental biology, it has relied on transient manipulations wild animals, lacked strategy for stable transgenesis. Here we build recent progress to develop more genetically tractable species, Lytechinus pictus , establish robust transgene integration method. Three commonly used transposons ( Minos, Tol2, piggyBac ) were tested non-autonomous transposition, using plasmids containing polyubiquitin promoter upstream H2B-mCerulean nuclear marker. Minos was only transposable element that resulted significant expression past metamorphosis. F 0 animals raised sexual maturity spawned determine germline integration, inheritance frequency, characterize patterns 1 progeny. The results demonstrated transmission through germline, first example transgenic urchin, indeed any echinoderm. This milestone paves way generation diverse resources will dramatically enhance utility, reproducibility, efficiency research. Significance Statement Transgenic tools are essential effective utilization models. Despite being an established not previously benefited from technology. study reports opens new avenues this organism.

Язык: Английский

Процитировано

0