Preliminary results and a theoretical perspective of co‑treatment using a miR‑93‑5p mimic and aged garlic extract to inhibit the expression of the pro‑inflammatory interleukin‑8 gene
Experimental and Therapeutic Medicine,
Год журнала:
2025,
Номер
29(4), С. 1 - 11
Опубликована: Фев. 25, 2025
The
coronavirus
disease‑19
(COVID‑19)
pandemic
has
been
a
very
significant
health
issue
in
the
period
between
2020
and
2023,
forcing
research
to
characterize
severe
acute
respiratory
syndrome
2
(SARS‑CoV‑2)
sequences
develop
novel
therapeutic
approaches.
Interleukin‑6
(IL‑6)
IL‑8
are
considered
targets
for
COVID‑19
emerging
evidence
suggested
that
microRNAs
(miRNAs/miRs)
serve
key
role
regulating
these
genes.
MiRNAs
short,
19‑25
nucleotides
length,
non‑coding
RNAs
regulate
gene
expression
at
post‑transcriptional
level
through
sequence‑selective
recognition
of
3'‑untranslated
region
(3'‑UTR)
regulated
mRNAs,
eventually
repressing
translation,
commonly,
via
mRNA
degradation.
For
example,
among
several
miRNAs
involved
regulation
'cytokine
storm',
miR‑93‑5p
can
inhibit
by
directly
targeting
3'‑UTR
mRNA.
In
addition,
Toll‑like
receptor‑4
(TLR4)
interleukin‑1
receptor‑associated
kinase
4
(IRAK4)
expression,
thus
affecting
nuclear
factor‑κB
(NF‑κB)
pathway
NF‑κB‑regulated
genes,
such
as
IL‑6,
IL‑1β
other
hyper‑expressed
genes
during
storm'.
present
study,
results
provided
preliminary
suggesting
miR‑93‑5p‑based
miRNA
therapeutics
could
be
combined
with
anti‑inflammatory
aged
garlic
extract
(AGE)
more
effectively
expression.
human
bronchial
epithelial
IB3‑1
cell
line
was
employed
experimental
model
system.
cells
were
stimulated
BNT162b2
vaccine
transfected
pre‑miR‑93‑5p
absence
or
presence
AGE,
verify
inhibitory
effects
on
BNT162b2‑induced
gene.
accumulation
assessed
RT‑qPCR;
release
protein
determined
Bio‑Plex
assay.
possible
applications
TLR4/NF‑κB
agents
(such
AGE)
treating
pathologies
hyperinflammatory
state,
COVID‑19,
cystic
fibrosis
diseases,
summarized.
Язык: Английский
Aged Garlic Extract (AGE) and Its Constituent S-Allyl-Cysteine (SAC) Inhibit the Expression of Pro-Inflammatory Genes Induced in Bronchial Epithelial IB3-1 Cells by Exposure to the SARS-CoV-2 Spike Protein and the BNT162b2 Vaccine
Molecules,
Год журнала:
2024,
Номер
29(24), С. 5938 - 5938
Опубликована: Дек. 16, 2024
Garlic
(Allium
sativum
L.)
is
a
species
of
the
onion
family
(Alliaceae)
widely
used
as
food
and
folk
medicine.
The
objective
this
study
was
to
determine
effects
AGE
(aged
garlic
extract)
on
pro-inflammatory
genes
relevant
COVID-19.
To
aim,
we
treated
bronchial
epithelial
IB3-1
cells
with
SARS-CoV-2
spike
protein
(S-protein)
or
COVID-19
BNT162b2
vaccine
in
absence
presence
AGE.
results
obtained
demonstrated
that
potent
inhibitor
S-protein-induced
expression
IL-1β,
IL-6
IL-8
genes.
Bio-Plex
analysis
reduced
release
IL-8,
which
were
highly
induced
by
S-protein.
No
inhibition
cells'
growth,
toxicity
pro-apoptotic
found
AGE-treated
cells.
one
major
constituents
(S-allyl
cysteine,
SAC)
studied
same
experimental
model
systems.
SAC
able
inhibit
extracellular
confirming
S-allyl-cysteine
responsible
for
inhibiting
Docking
experiments
suggest
possible
mechanism
action
an
interference
activity
Toll-like
receptors
(TLRs),
particularly
TLR4,
thereby
NF-κB-
NF-κB-regulated
genes,
such
These
both
deserve
further
efforts
verify
their
SARS-CoV-2-infected
Язык: Английский