Caco2/HT-29 In Vitro Cell Co-Culture: Barrier Integrity, Permeability, and Tight Junctions’ Composition During Progressive Passages of Parental Cells
Biology,
Год журнала:
2025,
Номер
14(3), С. 267 - 267
Опубликована: Март 6, 2025
Epithelial
linings
are
crucial
for
the
maintenance
of
physiological
barriers.
The
intestinal
epithelial
barrier
(IEB)
consists
enterocytes
through
tight
junctions
and
mucus-secreting
cells
can
undergo
modifications
throughout
life.
To
reproduce
as
closely
possible
IEB
main
features
over
time,
in
vitro
co-cultures
Caco2/HT-29
70/30
formed
by
parental
Caco2
HT-29
sub-cultivated
more
than
40
passages
were
set
up.
measurements
transepithelial
electrical
resistance
(TEER)
identified
two
populations:
TEER
(PC)
with
values
>
50
Ωcm2
fewer
passages,
leaky
(LC)
<
passages.
In
LC,
paracellular
permeability
increased
parallel.
By
immunofluorescence
Western
blot
analysis,
an
increase
claudin
2
was
observed
LC
vs.
PC,
no
differences
occludin
expression.
MUC-2
immunoreactivity
stronger
PC
LC.
also
showed
enhanced
vulnerability
to
TNFα+IFN-γ.
These
results
morpho-functional
reported
human
leaky/aged
gut
support
usefulness
our
cell
model
studying
molecular
processes
underlying
these
testing
drug/nutraceutical
treatments
ameliorate
aging.
Язык: Английский
Runchangningshen paste activates NLRP6 inflammasome-mediated autophagy to stimulate colonic mucin-2 secretion and modulates mucosal microbiota in functional constipation
World Journal of Gastroenterology,
Год журнала:
2025,
Номер
31(9)
Опубликована: Фев. 18, 2025
Runchangningshen
paste
(RCNSP)
is
a
made
of
four
medicinal
and
edible
homologous
Chinese
medicine
mixed
with
honey.
It
known
for
its
ability
to
nourish
yin
blood
as
well
loosen
the
bowel
relieve
constipation.
The
pathophysiology
functional
constipation
(FC)
associated
reduction
in
mucin-2
(MUC2)
secretion
microbial
dysbiosis.
To
investigate
underlying
mechanism
RCNSP
against
FC
through
MUC2
gut
mucosal
microbiota.
Ultra-performance
liquid
chromatography
tandem
mass
spectrometry
characterized
composition
elucidate
material
basis
action.
model
was
induced
via
loperamide
gavage
(16
mg/kg)
twice
daily
7
days.
Applying
defecation
function
gastrointestinal
motility
assess
severity.
Hematoxylin
eosin
Alcian
blue-periodic
acid-schiff
staining
analyzed
colonic
morphology.
Transmission
electron
microscope
used
observe
ultrastructure
goblet
cells
(GCs).
Immunofluorescence
colocalization,
quantitative
PCR,
western
blot
assessed
impact
on
gene
protein
expression
within
NLRP6/autophagy
pathway.
16S
rDNA
employed
sequence
contained
12
components
potential
laxative
effects.
enhanced
function,
accelerated
motility,
maintained
integrity.
treatment
significantly
increased
GC
abundance
production
while
preserving
ultrastructure.
At
molecular
level,
colocalized
key
regulatory
proteins
modulated
mRNA
expressions
Through
sequencing
analysis,
altered
microbiota
composition.
Specifically,
it
beneficial
bacterial
strains
reducing
harmful
ones.
Simultaneously,
reduced
butyrate-producing
bacteria
like
Proteobacteria,
Enterobacteriaceae,
Blautia,
Eubacterium
decreased
hydrogen
sulfide-producing
species,
such
Prevotellaceae.
also
bile
acid-inhibiting
g_Eubacter_coprostanoligenes_group
Erysipelotrichaceae
increasing
acid-producing
Colidextribacter.
Our
findings
suggested
that
ameliorated
dual
mechanism:
stimulated
by
activating
NLRP6
inflammasome-mediated
autophagy
Язык: Английский
Colonic bacterial community responding to selenium-enriched yeast supplementation associated with improved gut mucus function in growing-finishing pigs
BMC Microbiology,
Год журнала:
2025,
Номер
25(1)
Опубликована: Апрель 14, 2025
Selenium-enriched
yeast
(SeY),
a
high-quality
organic
source
of
selenium,
enhances
antioxidant
activity
and
intestinal
health
in
swine.
This
study
aims
to
evaluate
the
effects
varying
dietary
SeY
levels
on
morphology,
epithelial
mucus
production,
activity,
colonic
bacterial
communities
growing-finishing
pigs.
Thirty
90-day-old
Duroc×Landrace×Yorkshire
pigs
(average
body
weight
54.37±2.13
kg)
were
randomly
assigned
five
treatment
groups.
The
control
group
(CON)
was
fed
basal
diet,
while
other
four
groups
diet
supplemented
with
at
0.3,
1,
3,
5
mg/kg,
respectively,
for
an
80-day
feeding
trial.
results
showed
that
addition
0.3
mg/kg
increased
villus
height,
height/crypt
ratio,
production
ileum,
as
evidenced
by
increase
goblet
cell
number
thickness
(P
<
0.05).
Furthermore,
up-regulated
mRNA
expression
MUC-1,
claudin-1,
occludin,
ZO-1
genes
In
contrast,
high-dose
resulting
damage
mucosal
morphology.
Ileal
SOD
GSH-Px,
jejunal
GPX-1
GPX-4,
higher
response
Faecal
Se
excretion
dose-dependent
manner
led
significant
difference
beta
diversity
among
=
0.002)
decrease
concentrations
isobutyric
isovaleric
acids
when
compared
acetate,
propionate,
butyrate,
total
short-chain
fatty
positively
correlated
biomarker
genera
Agathobacter
(SeY
0.3mg/kg),
isobutyrate
isovalerate
negatively
Lactobacillus
0.3mg/kg)
accumulation
Alloprevotella
3mg/kg)
Prevotellaceae_UCG-001
5mg/kg)
Bacteroides
(CON),
Faecalibacterium
conclusion,
doses
have
beneficial
health,
whereas
prolonged
up
may
compromise
function
Язык: Английский
Enhancing food digestion and nutrient absorption through pre-digestion: Tailored strategies for special populations
Food & medicine homology.,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
The
human
body
relies
on
the
intake
and
utilization
of
nutrients
from
food
to
support
growth,
development,
daily
activities.
However,
certain
groups,
such
as
infants,
elderly,
patients
with
digestive
diseases
(e.g.,
chronic
atrophic
gastritis,
pancreatitis,
cystic
fibrosis)
experience
impaired
gastrointestinal
function
due
developmental
immaturity
or
physiological
decline,
leading
reduced
digestion
absorption
capacity.
This
has
resulted
in
emergence
pre-digested
foods.
Nonetheless,
research
nutritional
functional
properties
these
foods,
specifically
tailored
characteristics
special
populations,
remains
limited.
review
begins
an
overview
general
processes
digestion,
absorption,
fermentation
tract.
It
then
summarizes
systems
individuals
diseases,
while
highlighting
recent
advancements
regarding
effects
pre-digestion
capabilities
groups.
insights
presented
aim
provide
a
scientific
foundation
for
precise
design
development
healthy
foods
suited
populations.
Язык: Английский