Cold stress regulates accumulation of flavonoids and terpenoids in plants by phytohormone, transcription process, functional enzyme, and epigenetics

Critical Reviews in Biotechnology, Год журнала: 2022, Номер 43(5), С. 680 - 697

Опубликована: Июль 18, 2022

Plants make different defense mechanisms in response to environmental stresses. One common way is produce secondary metabolites. Temperature the main factor that regulates plant metabolites, especially flavonoids and terpenoids. Stress caused by temperature decreasing 4–10 °C conducive accumulation of However, mechanism under cold stress still lacks a systematic explanation. In this review, we summarize three aspects promoting terpenoids plants, is, affecting (1) content endogenous hormones, jasmonic acid abscisic acid; (2) expression level activity important transcription factors, such as bHLH MYB families. This aspect also includes post-translational modification factors stress; (3) key enzyme genes biosynthesis pathway, addition, rate-limiting glycosyltransferases are responsive stress. The understanding flavonoids, will contribute future research genetic engineering breeding, metabolism regulation, mining, synthetic biology.

Язык: Английский

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Diverse roles of MYB transcription factors in plants

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

MYB transcription factors (TFs), one of the largest TF families in plants, are involved various plant-specific processes as central regulators, such phenylpropanoid metabolism, cell cycle, formation root hair and trichome, phytohormones responses, reproductive growth abiotic or biotic stress responses. Here we summarized multiple roles explained molecular mechanisms TFs plant development adaptation. The exploration contributes to a better comprehension regulation environmental adaptability.

Язык: Английский

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Identification of PAL genes related to anthocyanin synthesis in tea plants and its correlation with anthocyanin content

Horticultural Plant Journal, Год журнала: 2021, Номер 8(3), С. 381 - 394

Опубликована: Дек. 24, 2021

The phenylalanine ammonia-lyase (PAL) gene family in tea plants (Camellia sinensis L.) encodes the enzyme that catalyzes first reaction of phenylpropane metabolic pathway. present study aimed to characterize PAL genes plants, and get better insights on CsPALs anthocyanins accumulation. Seven CsPAL were identified characterized by bioinformatics analysis. Systematic analysis was conducted for its phylogenetic relationship, structure, chromosomal location, protein conserved motifs based plant genome. cis-elements responsive light, abiotic stress, hormone, MYB-binding site. Furthermore, tissue-specific expression showed CsPAL4 expressed preferentially young leaves buds. Correlation performed purple-leaf with anthocyanin components, it suggested closely related different accumulated, especially cyanidin 3-O-galactoside, 3-O-glucoside, delphinidin 3-O-glucoside. Additionally, putative upstream regulation factors CsMYBs (CsMYB59, CsARR1, CsSRM1, CsMYB101, CsMYB52) CsbHLHs (CsbHLH104, CsbHLH3, CsbIM1, CsTCP14, CsPIF4) could bind promoter CsPALs, thereby activating transcription. This provides a theoretical basis further research elucidate functions genes.

Язык: Английский

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Comprehensive analysis of safflower R2R3-MYBs reveals the regulation mechanism of CtMYB76 on flavonol biosynthesis

Industrial Crops and Products, Год журнала: 2025, Номер 227, С. 120795 - 120795

Опубликована: Март 7, 2025

Язык: Английский

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Processing, chemical signature and food industry applications of Camellia sinensis teas: An overview

Food Chemistry X, Год журнала: 2021, Номер 12, С. 100160 - 100160

Опубликована: Ноя. 12, 2021

The plant Camellia sinensis is the source of different teas (white, green, yellow, oolong, black, and pu-ehr) consumed worldwide, which are classified by oxidation degree their bioactive compounds. sensory (taste, aroma, body drink) functional properties affected amount methylxanthines (caffeine theobromine), amino acids (l-theanine) reducing sugars in composition. Additionally, flavan-3-ols, mainly characterized epicatechins, catechins, derivatives, represent on average, 60% compounds teas. These secondary metabolites from widely recognized for antioxidant, anti-cancer, anti-inflammatory properties. Thus, extracts isolated have been increasingly used food industry. However, very susceptible to caused processing degradation under physiological conditions gastrointestinal digestion. In this context, new approaches/technologies developed preservation these This review presents main stages involved production following a description compounds, biological properties, stability bioaccessibility. Besides, updated view field science technology was provided focusing novel findings innovations published scientific literature over last five years.

Язык: Английский

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Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Five Solanaceae Species and Identification of Members Regulating Carotenoid Biosynthesis in Wolfberry

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(4), С. 2259 - 2259

Опубликована: Фев. 18, 2022

The R2R3-MYB is a large gene family involved in various plant functions, including carotenoid biosynthesis. However, this lacks comprehensive analysis wolfberry (Lycium barbarum L.) and other Solanaceae species. recent sequencing of the genome provides an opportunity for investigating organization evolutionary characteristics genes A total 610 were identified five species, 137 wolfberry. LbaR2R3-MYB grouped into 31 subgroups based on phylogenetic analysis, conserved structures, motif composition. Five groups only functionally divergent during evolution. Dispersed whole duplication events are critical expanding family. There 287 orthologous pairs between four selected RNA-seq expression level differential (DEGs) biosynthesis (CBGs) fruit development stages. highly expressed co-expressed with CBGs development. quantitative Real-Time (qRT)-PCR verified seven candidate genes. Thus, Lba11g0183 Lba02g01219 regulating This study elucidates evolution function

Язык: Английский

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High Light Intensity Triggered Abscisic Acid Biosynthesis Mediates Anthocyanin Accumulation in Young Leaves of Tea Plant (Camellia sinensis)

Antioxidants, Год журнала: 2023, Номер 12(2), С. 392 - 392

Опубликована: Фев. 6, 2023

There is increasing interest in the production and consumption of tea (Camellia sinensis L.) processed from purple–leaved cultivar due to their high anthocyanin content health benefits. However, how why seasonal changes affect accumulation young leaves still remains obscured. In this study, abscisic acid (ABA) contents Zifuxing 1 (ZFX1), a with new shoots turning purple Wuyi Mountain, key region China, were monitored over four seasons. Young produced September highly purplish, which was accompanied higher ABA contents. Among environmental factors, light intensity particular closely correlated A shade experiment also indicated that significantly decreased after 168 h growth under 75% shade, but treatment conditions sustained content. To confirm involvement modulation accumulation, anthocyanin, carotenoids, chlorophyll, ABA, jasmonic (JA), salicylic (SA) cultivars, including ZFX1, Zijuan (ZJ), wherein are completely purple, Rougui (RG) Fudingdabaicha (FDDB) green, analyzed, antioxidant activities leaf extracts tested. Results showed not other tested hormones, cultivars. Cultivars exhibited activities. Subsequently, ZFX1 plants grown full sun treated fluridone (Flu), an inhibitor. elevated level chlorophyll The reverse true those Flu. pursue better understanding RNA–Seq used analyze transcript differences among ABA– or Flu–treated untreated plants. differentially expressed genes Flu mainly signal sensing metabolism–related genes, accumulation-related light–responsive regulatory MYB transcription factors. Taking all results into account, model for proposed: caused reactive oxygen stress, triggered biosynthesis ABA; interactions such as MYB-enhanced limited carotenoid accumulation; transport vacuoles resulting purplish coloration. Further research warranted test model.

Язык: Английский

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Anthocyanin metabolism and its differential regulation in purple tea (Camellia sinensis)

Plant Physiology and Biochemistry, Год журнала: 2023, Номер 201, С. 107875 - 107875

Опубликована: Июль 9, 2023

Язык: Английский

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Genome wide characterization of R2R3 MYB transcription factor from Apocynum venetum revealed potential stress tolerance and flavonoid biosynthesis genes

Genomics, Год журнала: 2022, Номер 114(2), С. 110275 - 110275

Опубликована: Янв. 31, 2022

MYB transcription factors are crucial in regulating stress tolerance and expression of major genes involved flavonoid biosynthesis. The functions MYBs is well explored a number plants, yet no study reported Apocynum venetum. We identified total 163 candidates, that comprised 101 (61.96%) R2R3, 6 3R, 1 4R 55 1R. Syntenic analysis A. venetum R2R3 (AvMYBs) showed highest orthologous pairs with Vitis vinifera followed by Arabidopsis thaliana among the four species evaluated. Thirty segmental duplications tandem were obtained AvMYB gene signifying their role family expansion. Nucleotide substitution (Ka/Ks) AvMYBs to be under influence strong purifying selection. Expression selected low temperature cadmium stresses resulted identification AvMYB48, AvMYB97, AvMYB8, AvMYB4 as potential responsive AvMYB10 AvMYB11 addition, proanthocyanidin biosynthesis regulatory which consistent annotated homologues Arabidopsis. Tissue specific profile further supported qPCR result. higher transcript levels root, stem leaf like for example, was downregulated such level AvMYB48 had upregulated both stresses. Transcriptome phylogenetic analyses suggested AvMYB42 regulator anthocyanin Thus, this provided valuable information on AvR2R3-MYB respect

Язык: Английский

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Integrated transcriptome and metabolome provide insights into flavonoid biosynthesis in 'P113', a new purple tea of <i>Camellia tachangensis</i>

Beverage Plant Research, Год журнала: 2023, Номер 3(1), С. 1 - 11

Опубликована: Янв. 1, 2023

Plants typically exhibit the purple phenomenon as a result of an increase in flavonoids and anthocyanins. A new tea germplasm 'P113' was recently selected from Camellia tachangensis, which is tender shoots. In present study, integrated transcriptome metabolome were used to analyze flavonoid metabolite components genes involved biosynthesis 'P113'. total 86 metabolites identified, including 70 significantly differential (p < 0.05) they enriched three metabolic pathways ko00941, ko00942 ko00944 through KEGG pathway analysis. 136 obtained transcriptomic 53 differentially expressed developmental The correlation between profiling transcriptome, protein interactions suggested that transcription factor MYB12 glycosyltransferase UGT78D2 had good facilitation on potential underlie coloration shoots are clarified this study. It also lays groundwork for identifying color variation offers theoretical framework creation use distinctive genetic resources breeding cultivars.

Язык: Английский

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