Proceedings of the National Academy of Sciences,
Год журнала:
2025,
Номер
122(11)
Опубликована: Март 10, 2025
Recurrent
implantation
failure
(RIF)
is
a
devastating
condition
that
leaves
many
undergoing
fertility
treatment
childless.
The
human
endometrium
receptive
to
blastocyst
for
brief
period,
the
window
of
implantation.
Critical
knowledge
underpinning
biological
processes
leading
RIF,
essential
effective
treatment,
lacking.
We
employed
spatial
transcriptomics
define
region-
and
cell-type-specific
differences
in
endometrial
gene
expression
luteinizing
hormone
timed
biopsies
between
women
with
RIF
(n
=
8)
fertile
controls
(FC)
8).
Differentially
expressed
genes
(DEGs)
were
identified
when
comparing
regions
FC
(685
luminal
epithelium,
293
glandular
419
subluminal
stroma,
264
functionalis
1,125
stromal
CD45
+
leukocytes,
1,049
CD56
leukocytes).
Only
57
DEGs
common
all
subregions
cell
types,
which
highlights
multiple
are
lost
examined
as
single
entity.
When
RIF-specific
leveraged
against
from
mouse
genetic
models,
associated
aberrant
embryo
phenotypes
observed,
mostly
immune
populations.
Dysregulated
pathways
specific
included
“WNT
signaling
pathway,”
altered
stroma.
“Response
estradiol”
“ovulation
cycle”
dysregulated
In
silico
drug
screening
potential
compounds
can
reverse
profile
(e.g.,
raloxifene,
bisoprolol).
Our
findings,
well-characterized
cohort,
highly
endorse
consideration
each
region
type
separate
entities.
Ignoring
individual
composite
populations
will
overlook
important
aberrations,
forego
targets,
lead
research
waste
pursuing
clinically
irrelevant
options.
hLife,
Год журнала:
2024,
Номер
2(7), С. 325 - 341
Опубликована: Янв. 25, 2024
Drug-induced
liver
injury
(DILI)
remains
a
serious
problem
in
clinics
for
both
diagnoses
and
treatment
decisions.
It
is
result
of
accumulated
drugs
human
bodies
metabolized
into
toxic
constituents
generating
reactive
metabolites,
then
arise
initial
consequences
oxidative
stress,
organelle
stress
responses
lethal
(liver
necrosis
or
apoptosis).
However,
the
idiosyncratic
nature
DILI
complexes
its
mechanistic
studies
still
little
known
potential
etiopathogenesis
certain.
Single
cell
omics
technology
approaches
serve
as
powerful
tools
investigating
cellular
heterogeneity
relationships
from
measurements
up
to
millions
individual
cells
at
an
unprecedented
resolution,
which
are
achieved
by
advances
genome,
epigenome,
transcriptome,
proteome,
metabolism
technologies.
As
contains
heterogeneous
types
distinct
spatial,
molecular,
functional
properties,
they
interact
with
each
other
proceed
type-specific
reprogramming
play
irreplaceable
role
properties
upon
encountering
insults.
omics,
especially
single
transcriptomics
proteomics,
have
been
utilized
exploring
mechanisms
prediction
risk
factors.
In
this
review,
we
discuss
recent
development
future
perspectives
omics-based
technologies
DILI-related
research.
Journal of Translational Medicine,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 4, 2025
CXCL14
is
a
highly
conserved
chemokine
expressed
in
various
cell
types,
playing
crucial
roles
both
physiological
and
pathological
processes,
including
immune
regulation
tumorigenesis.
Recently,
the
role
of
tumors
has
attracted
considerable
attention.
However,
previous
pan-cancer
studies
have
reported
inconsistencies
regarding
effects
on
tumors,
particularly
concerning
its
expression
levels
tumor
tissues
influence
phenotypes
cancer
cells.
This
variability
believed
to
stem
from
context-dependent
nature
CXCL14,
as
different
sources
secretion
within
distinct
microenvironments
may
mediate
diverse
biological
effects.
Such
phenomena
also
been
observed
prostate
research.
Despite
foundational
understanding
cancer,
there
remains
lack
comprehensive
reviews
summarizing
specific
this
systematically
analyzing
reasons
behind
complex
Therefore,
article
aims
discuss
microenvironment
explore
future
research
directions
potential
applications.
Proteomes,
Год журнала:
2025,
Номер
13(1), С. 3 - 3
Опубликована: Янв. 13, 2025
Idiopathic
pulmonary
fibrosis
(IPF)
is
a
progressive
lung
disease
characterized
by
repetitive
alveolar
injuries
with
excessive
deposition
of
extracellular
matrix
(ECM)
proteins.
A
crucial
need
in
understanding
IPF
pathogenesis
identifying
cell
types
associated
histopathological
regions,
particularly
local
centers
known
as
fibroblast
foci.
To
address
this,
we
integrated
published
spatial
transcriptomics
and
single-cell
RNA
sequencing
(scRNA-seq)
adopted
the
Query
method
Overlap
to
determine
type
enrichments
regions.
Distinct
are
highly
foci,
transitional
2
aberrant
KRT5-/KRT17+
(KRT:
keratin)
epithelial
cells
morphologically
normal
alveoli
human
lungs.
Furthermore,
employed
laser
capture
microdissection-directed
mass
spectrometry
profile
By
comparing
another
similar
dataset,
common
differentially
expressed
proteins
enriched
pathways
related
ECM
structure
organization
collagen
processing
were
identified
Importantly,
enrichment
results
from
innovative
proteomics
scRNA-seq
data
integration
accord
those
integration,
supporting
capability
versatility
entire
approach.
In
summary,
multi-omics
identify
disease-associated
potential
targets
for
novel
therapies
intervention.
The
approach
can
be
further
applied
other
areas
heterogeneity.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 995 - 995
Опубликована: Янв. 24, 2025
Spatial
transcriptomics
has
transformed
our
understanding
of
gene
expression
by
preserving
the
spatial
context
within
tissues.
This
review
focuses
on
application
in
human
cardiac
tissues,
exploring
current
technologies
with
a
focus
commercially
available
platforms.
We
also
highlight
key
studies
utilizing
to
investigate
development,
electro-anatomy,
immunology,
and
ischemic
heart
disease.
These
demonstrate
how
can
be
used
conjunction
other
omics
provide
more
comprehensive
picture
health
Despite
its
transformative
potential,
comes
several
challenges
that
limit
widespread
adoption
broader
application.
By
addressing
these
limitations
fostering
interdisciplinary
collaboration,
potential
become
an
essential
tool
cardiovascular
research.
hope
this
serves
as
practical
guide
for
researchers
interested
adopting
transcriptomics,
particularly
those
limited
prior
experience,
providing
insights
into
technologies,
applications,
considerations
successful
implementation.
