Analysis of mutually exclusive expression in cancer cells identifies a previously unknown intergenic regulatory paradigm DOI
Ling Tang,

Yuzhe Hu,

Chao Wang

и другие.

FEBS Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Mutual exclusion of gene expression has received limited attention. Gene (expression) plasticity analysis provides an efficient way to identify highly plastic genes (HPGs) based on changes in rank. In this study, we quantitatively measured the 19 961 protein‐coding 24 human cancer cell lines and identified HPGs these cells. By comparing methods, showed that virtual sorting cosine similarity, rather than Pearson Spearman rank correlations, are suitable for mutual exclusion. Mutually exclusive pairs were each type. Experimental validation thiol methyltransferase 1B ( TMT1B ; also known as METTL7B ) CD274 molecule PD‐L1 mutually exclusively expressed at either mRNA or protein level. negatively regulated several types, JAK/STAT3 pathway was involved. Knockdown Huh7 cells inhibited interleukin 2 (IL‐2) secretion by Jurkat co‐culture experiments, inhibition blocked anti‐PD‐L1 antibodies. Therefore, study method expressional implies a newly intergenic regulatory paradigm.

Язык: Английский

VSTM1/SIRL‐1: An Inhibitory Pattern Recognition Receptor Regulating Myeloid Cells DOI Creative Commons

Maaike F.M. Koops,

Linde Meyaard

European Journal of Immunology, Год журнала: 2025, Номер 55(2)

Опубликована: Фев. 1, 2025

Innate immune cells express a plethora of inhibitory receptors, many which recognize molecular patterns. An appropriate balance between signaling via activating and pattern recognition receptors is important for proper response while preventing immunopathology. V-set transmembrane domain containing 1 (VSTM1), also known as signal receptor on leukocytes-1 (SIRL-1), an expressed myeloid cells. VSTM1 can modulate the function cells, by inhibiting reactive oxygen species neutrophil extracellular trap formation. recognizes shared patterns both from endogenous microbial origin, defining it receptor. involved in various pathological conditions, including autoimmune disorders cancer, its restricted expression highlights potential specific therapeutic target. This review summarizes characteristics health disease.

Язык: Английский

Процитировано

0
Analysis of mutually exclusive expression in cancer cells identifies a previously unknown intergenic regulatory paradigm DOI
Ling Tang,

Yuzhe Hu,

Chao Wang

и другие.

FEBS Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Mutual exclusion of gene expression has received limited attention. Gene (expression) plasticity analysis provides an efficient way to identify highly plastic genes (HPGs) based on changes in rank. In this study, we quantitatively measured the 19 961 protein‐coding 24 human cancer cell lines and identified HPGs these cells. By comparing methods, showed that virtual sorting cosine similarity, rather than Pearson Spearman rank correlations, are suitable for mutual exclusion. Mutually exclusive pairs were each type. Experimental validation thiol methyltransferase 1B ( TMT1B ; also known as METTL7B ) CD274 molecule PD‐L1 mutually exclusively expressed at either mRNA or protein level. negatively regulated several types, JAK/STAT3 pathway was involved. Knockdown Huh7 cells inhibited interleukin 2 (IL‐2) secretion by Jurkat co‐culture experiments, inhibition blocked anti‐PD‐L1 antibodies. Therefore, study method expressional implies a newly intergenic regulatory paradigm.

Язык: Английский

Процитировано

0