Acetate metabolism during xylose fermentation enhances 3-hydroxypropionic acid production in engineered acid-tolerantIssatchenkia orientalis DOI
Deokyeol Jeong, Da Hye Lee, Junli Liu

и другие.

Опубликована: Апрель 17, 2025

Abstract Efficient bioconversion of acetate-rich lignocellulosic biomass into value-added chemicals remains a major challenge due to the toxicity acetic acid. In this study, we engineered an acid-tolerant Issatchenkia orientalis strain (IoDY01H) capable producing 3-hydroxypropionic acid (3-HP), key bioplastic precursor, from glucose, xylose, and acetate. Using Cas9-based genome editing system with hygromycin B resistance marker, introduced heterologous genes encoding xylose utilization β-alanine-based 3-HP biosynthetic pathways I. genome. Metabolomic analysis revealed that acetate supplementation redirected metabolic flux toward amino lipid metabolism while reducing TCA cycle intermediates. Acetate enhanced production by promoting accumulation β-alanine, but also β-alanine–pyruvate aminotransferase as bottleneck under acidic conditions. pretreated hemp stalk hydrolysate feedstock, achieved titer 8.7 g/L via separate hydrolysis fermentation (SHF), outperforming simultaneous saccharification (SSF). These findings demonstrate feasibility using non-conventional yeast highlight promising microbial chassis for industrial bioconversion. Graphical abstract Highlights Engineered co-utilized produce 3-HP. addition during fermentation. biosynthesis pathways. SHF.

Язык: Английский

Acetate metabolism during xylose fermentation enhances 3-hydroxypropionic acid production in engineered acid-tolerantIssatchenkia orientalis DOI
Deokyeol Jeong, Da Hye Lee, Junli Liu

и другие.

Опубликована: Апрель 17, 2025

Abstract Efficient bioconversion of acetate-rich lignocellulosic biomass into value-added chemicals remains a major challenge due to the toxicity acetic acid. In this study, we engineered an acid-tolerant Issatchenkia orientalis strain (IoDY01H) capable producing 3-hydroxypropionic acid (3-HP), key bioplastic precursor, from glucose, xylose, and acetate. Using Cas9-based genome editing system with hygromycin B resistance marker, introduced heterologous genes encoding xylose utilization β-alanine-based 3-HP biosynthetic pathways I. genome. Metabolomic analysis revealed that acetate supplementation redirected metabolic flux toward amino lipid metabolism while reducing TCA cycle intermediates. Acetate enhanced production by promoting accumulation β-alanine, but also β-alanine–pyruvate aminotransferase as bottleneck under acidic conditions. pretreated hemp stalk hydrolysate feedstock, achieved titer 8.7 g/L via separate hydrolysis fermentation (SHF), outperforming simultaneous saccharification (SSF). These findings demonstrate feasibility using non-conventional yeast highlight promising microbial chassis for industrial bioconversion. Graphical abstract Highlights Engineered co-utilized produce 3-HP. addition during fermentation. biosynthesis pathways. SHF.

Язык: Английский

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