het-Ballorecognition inPodospora anserinais determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein DOI Creative Commons
Corinne Clavé,

Sonia Dheur,

S. Lorena Ament‐Velásquez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 19, 2023

Abstract Filamentous fungi display allorecognition genes that trigger regulated cell death (RCD) when strains of unlike genotype fuse. Podospora anserina is one several model species for the study this process termed heterokaryon or vegetative incompatibility. Incompatibility restricts transmission mycoviruses between isolates. In P. , genetic analyses have identified nine incompatibility loci, het loci. Here we set out to clone controlling het-B displays two incompatible alleles, het-B1 and het-B2 . We find locus encompasses adjacent genes, Bh Bp exist as highly divergent allelic variants ( Bh1/Bh2 Bp1/Bp2 ) in haplotypes. encodes a protein with an N-terminal HET domain, inducing domain bearing homology Toll/interleukin-1 receptor (TIR) domains C-terminal predicted lectin fold. The product homologous PII-like proteins, family small trimeric proteins acting sensors adenine nucleotides bacteria. show although system appears genetically allelic, fact determined by non-allelic Bh1/Bp2 interaction while reciprocal Bh2/Bp1 plays no role fold BH determines recognition specificity. Population studies genome indicate under balancing selection trans-species polymorphism, highlighting evolutionary significance addition emphasizing anew central TIR-like fungal RCD, identifies novel players completes characterization entire gene species. Author summary Many cellular life forms systems protect individuality discriminate conspecific self from non-self. filamentous fungi, fusion events are check specific upon detection restrict mycovirus parasitism. good form allorecognition, harbors Previous revealed these can be immune functions other phyla including bacteria, plants animals. cloned het-B, last remained found it complex comprising (related TIR domains) BP known bacterial metabolite sensors. Intriguingly, despite apparent allelism, dictated Bh/Bp interaction. This stresses reoccurring involvement RCD signs completion loci species, enabling comparative analysis different architectures underlying allorecognition.

Язык: Английский

FungANI, a BLAST-based program for analyzing average nucleotide identity (ANI) between two fungal genomes, enables easy fungal species delimitation DOI

Christophe Lalanne,

Philippe Silar

Fungal Genetics and Biology, Год журнала: 2025, Номер 177, С. 103969 - 103969

Опубликована: Янв. 31, 2025

Язык: Английский

Процитировано

1
Organic fertilizers increase the proportion of saprotrophs favoring soil nitrification under medicinal plants Fritillaria thunbergii DOI

Jianhang Du,

Yefei Yu,

Caixian Tang

и другие.

Industrial Crops and Products, Год журнала: 2024, Номер 219, С. 119129 - 119129

Опубликована: Июль 9, 2024

Язык: Английский

Процитировано

5
Huge genetic diversity ofSchizothecium tetrasporum(Wint.). N. Lundq.: delimitation of 18 species distributed into three complexes through genome sequencing DOI Creative Commons

Elsa De Filippo,

Valérie Gautier,

Christophe Lalanne

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Abstract Analyses of the genetic diversity well-studied fungi Sordariales order, such as Neurospora spp. and Podospora anserina (syn. Triangularia ), have shown that species classically defined by morphology are often complexes cryptic species. Here, we report on delimitation among 76 strains producing mycelium sexual reproductive structures identical to those pseudo-homothallic Schizothecium tetrasporum Neoschizothecium ). Their whole genomes were sequenced well six closely related but eight-spored asci instead four-spored ones. The clustering based Average Nucleotide Identity (ANI) between identified eighteen grouped into three clades, which further supported a phylogenetic tree constructed with genome Single Polymorphisms (SNPs). Based their contrasting breeding systems large evolutionary distances, considered clades distinct complexes. Indeed, two them, pseudotetrasporum complexes, contains asci, while third one, named octosporum , heterothallic asci. Surprisingly it was nestled Our data reveals thus huge morpho-species convergent evolution pseudo-homothallism or reversion heterothallism within An epitype for sensus stricto is seventeen new formally described.

Язык: Английский

Процитировано

0
Phylogenomic insights into the taxonomy, ecology, and mating systems of the lorchel family Discinaceae (Pezizales, Ascomycota) DOI
Alden C. Dirks, Andrew S. Methven, Andrew N. Miller

и другие.

Molecular Phylogenetics and Evolution, Год журнала: 2025, Номер unknown, С. 108286 - 108286

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
A tale of too many trees: a conundrum for phylogenetic regression DOI Creative Commons

Richard H. Adams,

Jenniffer Roa Lozano,

Mataya Duncan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 20, 2024

A bstract Just exactly which tree(s) should we assume when testing evolutionary hypotheses? This question has plagued comparative biologists for decades. Given a perfectly estimated tree (if this is even possible in practice), seldom know with certainty whether such truly best (or adequate) to represent the history of our studied traits. Regardless certainty, choosing required all phylogenetic methods. Yet, conflict and error are ubiquitous modern biology, still learning about their dangers hypotheses. Here investigated consequences gene tree-species mismatch regression presence incomplete lineage sorting. Our simulation experiments reveal excessively high false positive rates mismatched both small large trees, simple complex traits, known phylogenies. In some cases, find evidence directionality error: incorrectly assuming species traits that evolved according sometimes fares worse than opposite. To explore difficult yet realistic scenarios, also used rather trees conduct case studies, as well an expansive expression dataset investigate arguably best-case scenario one may have better chance match trait. Though never meant be panacea ail methods, found promise application robust estimator potential, albeit imperfect, solution issues raised by mismatch, perhaps offering path forward. Collectively, results emphasize importance careful study design highlighting need fully appreciate role adequate modeling

Язык: Английский

Процитировано

3
Comparative genomic analysis of thermophilic fungi reveals convergent evolutionary adaptations and gene losses DOI Creative Commons
Andrei Stecca Steindorff, María Victoria Aguilar Pontes, Aaron Robinson

и другие.

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Сен. 12, 2024

Abstract Thermophily is a trait scattered across the fungal tree of life, with its highest prevalence within three families (Chaetomiaceae, Thermoascaceae, and Trichocomaceae), as well some members phylum Mucoromycota. We examined 37 thermophilic thermotolerant species 42 mesophilic for this study identified thermophily ancestral state all prominent fungi. Thermophilic genomes were found to encode various thermostable enzymes, including carbohydrate-active enzymes such endoxylanases, which are useful many industrial applications. At same time, overall gene counts, especially in responsible microbial defense secondary metabolism, reduced thermophiles compared mesophiles. also reduction core genome size both Chaetomiaceae family Eurotiomycetes class. The Gene Ontology terms lost fungi include primary transporters, UV response, O-methyltransferases. Comparative genomics analysis revealed higher GC content third base codons (GC3) lower effective number than Furthermore, using Support Vector Machine classifier, we several Pfam domains capable discriminating between mesophiles 94% accuracy. Using AlphaFold2 predict protein structures endoxylanases (GH10), built similarity network based on structures. that disulfide bonds appears important structure, clusters correlate optimal activity temperature. Thus, comparative offers new insights into biology, adaptation, evolutionary history while providing parts list bioengineering

Язык: Английский

Процитировано

3
het-B allorecognition in Podospora anserina is determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein DOI Creative Commons
Corinne Clavé,

Sonia Dheur,

S. Lorena Ament‐Velásquez

и другие.

PLoS Genetics, Год журнала: 2024, Номер 20(2), С. e1011114 - e1011114

Опубликована: Фев. 12, 2024

Filamentous fungi display allorecognition genes that trigger regulated cell death (RCD) when strains of unlike genotype fuse. Podospora anserina is one several model species for the study this process termed heterokaryon or vegetative incompatibility. Incompatibility restricts transmission mycoviruses between isolates. In P . , genetic analyses have identified nine incompatibility loci, het loci. Here we set out to clone controlling het-B displays two incompatible alleles, het-B1 and het-B2 We find locus encompasses adjacent genes, Bh Bp exist as highly divergent allelic variants ( Bh1/Bh2 Bp1/Bp2 ) in haplotypes. encodes a protein with an N-terminal HET domain, inducing domain bearing homology Toll/interleukin-1 receptor (TIR) domains C-terminal predicted lectin fold. The product homologous PII-like proteins, family small trimeric proteins acting sensors adenine nucleotides bacteria. show although system appears genetically allelic, fact determined by non-allelic Bh1/Bp2 interaction while reciprocal Bh2/Bp1 plays no role fold BH determines recognition specificity. Population studies genome indicate under balancing selection trans-species polymorphism, highlighting evolutionary significance addition emphasizing anew central TIR-like fungal RCD, identifies novel players completes characterization entire gene species.

Язык: Английский

Процитировано

2
Arcopilins: A New Family of Staphylococcus aureus Biofilm Disruptors from the Soil Fungus Arcopilus navicularis DOI Creative Commons
Esteban Charria‐Girón,

Haoxuan Zeng,

Tatiana E. Gorelik

и другие.

Journal of Medicinal Chemistry, Год журнала: 2024, Номер 67(17), С. 15029 - 15040

Опубликована: Авг. 14, 2024

Biofilms represent a key challenge in the treatment of microbial infections; for instance, Staphylococcus aureus causes chronic or fatal infections by forming biofilms on medical devices. Herein, fungus Arcopilus navicularis was found to produce novel family PKS-NRPS metabolites that are able disrupt preformed S. aureus. Arcopilins A–F (1–6), tetramic acids, and arcopilin G (7), 2-pyridone, were elucidated using HR-ESI-MS one-dimensional (1D) two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy. Their absolute configuration established synthesis MPTA-esters 2, analysis 1H–1H coupling constants, ROESY correlations, along with comparison crystal structure 7. Arcopilin A (1) not only effectively disrupts but also potentiates activity gentamicin vancomycin up 115- 31-fold times, respectively. Our findings demonstrate potential application arcopilins conjugated caused antibiotics unable biofilms.

Язык: Английский

Процитировано

1
Pinibarreniales, a new order of Sordariomycetes from pine barrens ecosystem DOI
Jing Luo, Emily Walsh,

Alexis Faulborn

и другие.

Mycologia, Год журнала: 2024, Номер 116(5), С. 835 - 847

Опубликована: Июль 3, 2024

, sp. nov. isolated from the roots of highbush blueberry in New Jersey Pine Barrens, is described and illustrated. Based on multigene phylogenetic analysis, as well morphological ecological characteristics, Pinibarreniales Pinibarreniaceae are established to accommodate this novel lineage Sordariomycetidae, Sordariomycetes. Pinibarreniales, Tracyllalales, Vermiculariopsiellales proposed be included subclass Sordariomycetidae. likely have a wide distribution forms association with Ericaceae plants that live acidic oligotrophic environments because its DNA barcode matches environmental sequences other independent studies. The plant-fungal interaction experiment revealed negative impacts

Язык: Английский

Процитировано

0
het-Ballorecognition inPodospora anserinais determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein DOI Creative Commons
Corinne Clavé,

Sonia Dheur,

S. Lorena Ament‐Velásquez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 19, 2023

Abstract Filamentous fungi display allorecognition genes that trigger regulated cell death (RCD) when strains of unlike genotype fuse. Podospora anserina is one several model species for the study this process termed heterokaryon or vegetative incompatibility. Incompatibility restricts transmission mycoviruses between isolates. In P. , genetic analyses have identified nine incompatibility loci, het loci. Here we set out to clone controlling het-B displays two incompatible alleles, het-B1 and het-B2 . We find locus encompasses adjacent genes, Bh Bp exist as highly divergent allelic variants ( Bh1/Bh2 Bp1/Bp2 ) in haplotypes. encodes a protein with an N-terminal HET domain, inducing domain bearing homology Toll/interleukin-1 receptor (TIR) domains C-terminal predicted lectin fold. The product homologous PII-like proteins, family small trimeric proteins acting sensors adenine nucleotides bacteria. show although system appears genetically allelic, fact determined by non-allelic Bh1/Bp2 interaction while reciprocal Bh2/Bp1 plays no role fold BH determines recognition specificity. Population studies genome indicate under balancing selection trans-species polymorphism, highlighting evolutionary significance addition emphasizing anew central TIR-like fungal RCD, identifies novel players completes characterization entire gene species. Author summary Many cellular life forms systems protect individuality discriminate conspecific self from non-self. filamentous fungi, fusion events are check specific upon detection restrict mycovirus parasitism. good form allorecognition, harbors Previous revealed these can be immune functions other phyla including bacteria, plants animals. cloned het-B, last remained found it complex comprising (related TIR domains) BP known bacterial metabolite sensors. Intriguingly, despite apparent allelism, dictated Bh/Bp interaction. This stresses reoccurring involvement RCD signs completion loci species, enabling comparative analysis different architectures underlying allorecognition.

Язык: Английский

Процитировано

0