Target Enrichment Data Uncovers Rapid Radiation, Whole Genome Duplication, and Extensive Hybridization in Slipper Orchid GenusCypripediumL. DOI Open Access

Loudmila Jelinscaia Lagou,

Gudrun Kadereit, Diego F. Morales‐Briones

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 27, 2024

Background and Aims Cypripedium is the most widespread morphologically diverse genus of slipper orchids. Despite several published phylogenies, topology monophyly its infrageneric taxa remained uncertain. Here, we aimed to reconstruct a robust section-level phylogeny explore evolutionary history using target capture data for first time. Methods We used orchid-specific bait set Orchidaceae963 in combination with transcriptomic based on 913 nuclear loci, covering all 13 sections. Subsequently, investigated discordance among chloroplast trees, estimated divergence times ancestral ranges, searched anomaly zones, polytomies, diversification rate shifts, identified potential gene (genome) duplication hybridization events. Key Results All sections were recovered as monophyletic, contrary two subsections within sect. . The subclades this section did not correspond but matched geographic distribution their species. Additionally, discovered high levels short backbone branches , which can be attributed events detected phylogenetic network analyses, incomplete lineage sorting caused by rapid radiation. Our biogeographic analysis suggested Neotropical origin during Oligocene (∼30 Ma), potentially hybrid spreading Old World Early Miocene (∼22 Ma). radiation at likely occurred Southeast Asia around Middle Climatic Transition (∼15–13 followed independent dispersals back New World. Moreover, Pliocene-Quaternary glacial cycles may have contributed further speciation reticulate evolution Conclusions study provided novel insights into high-throughput molecular data, shedding light dynamics diversity patterns from present.

Язык: Английский

Unveiling a Meaningful Form of Cypripedium × ventricosum Sw. (Cypripedioideae, Orchidaceae) from Changbai Mountain, China: Insights from Morphological, Molecular, and Plastome Analyses DOI Creative Commons
Ying Li, Xi Lu, Shuang Li

и другие.

Plants, Год журнала: 2025, Номер 14(5), С. 772 - 772

Опубликована: Март 3, 2025

A Cypripedium plant was discovered in Wangqing County, Changbai Mountain, Jilin Province. This newly of not only inhabits the same natural habitat as calceolus L. and × ventricosum Sw. but also has a morphology intermediate between that C. ventricosum. Its dorsal sepals, petals, synsepal are similar to those calceolus, while color its lip is For purpose distinguishing from other plants, we provisionally named it W1. To further verify taxonomic status W1, introduced three identified forms conducted molecular biology analyses with The confirmed relationship W1 ventricosum, phylogenetic analysis nuclear region demonstrated close calceolus. Collectively, morphological evidence indicates product backcross Although shows differences typical can still be considered form We investigated chloroplast genome this determined total length 196,850 bp. contains 132 genes, including 87 protein-coding 37 tRNA 8 rRNA genes. By analyzing position study clarified relationships among taxa characteristics, laying foundation for research on orchid evolution species conservation.

Язык: Английский

Процитировано

1
Origin and biogeographical history of Palaeotropical Piper (Piperaceae): multiple long-distance dispersals from South America initiated during the Middle Eocene climatic optimum DOI
Dominik Metschina, Luiz Augusto Cauz‐Santos, Maarten J. M. Christenhusz

и другие.

Botanical Journal of the Linnean Society, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Abstract Piper is the largest genus of Piperaceae and second in angiosperms, but biogeography timing its global radiation has not yet been fully investigated. Here, we carried out phylogenetic analyses with a molecular clock to provide framework examine divergence times dispersal patterns genus, focus on Palaeotropical groups. Target enrichment produced 269 genes for 122 samples Piperaceae. For Piper, included species representing all geographic regions, most subgenera/sections, clades previously identified studies. We used maximum likelihood (RAxML, IQ-Tree) coalescent (ASTRAL-III) produce trees optimized branch lengths tree provided time estimates using treePL calibrated Cretaceous fossil P. margaritae Martínez-A. age 90.8 Myr, derived from previous estimate crown age. BioGeoBEARS was biogeographic analyses. generally well-supported which there good support (Verhuellia ((Manekia + Zippelia) (Piper Peperomia))). Intercontinental dispersals South America started during Middle Eocene climatic optimum (c. 40 Ma), first southern Africa Pacific islands, followed by New Guinea, Southeast Asia, Philippines. In dispersed diversified across Wallace Lydekker’s lines multiple times. Despite being only four endemic species, mainland colonized twice, it appears never there.

Язык: Английский

Процитировано

0
Persistent Habitat Instability and Patchiness, Sexual Attraction, Founder Events, Drift and Selection: A Recipe for Rapid Diversification of Orchids DOI Creative Commons
James D. Ackerman, Raymond L. Tremblay, Tatiana Arias

и другие.

Plants, Год журнала: 2025, Номер 14(8), С. 1193 - 1193

Опубликована: Апрель 11, 2025

Orchidaceae is one of the most species-rich families flowering plants, with current diversity having evolved within last 5 My. Patterns associated species richness and rapid diversification have been identified but not often evolutionary processes. We review frequently correlates suggest that processes rate by which they occur vary geographically are largely dependent on persistent pulses habitat instabilities, especially for epiphytes. Aggressive orogenesis creates fragmented habitats while global climatic cycles exacerbate ecological instabilities. The need repeated dispersal results in frequent founder events, sets stage allopatric via bouts genetic drift natural selection. allopatry requirement can be bypassed pollination systems involving flowers attracting pollinators through production sex signaling semiochemicals. drift-selection model diversification, coupled instability throughout geological time scales, likely components a multifactorial process leading to rapid, recent this family.

Язык: Английский

Процитировано

0
Comparative Analysis of the Chloroplast Genomes of Cypripedium: Assessing the Roles of SSRs and TRs in the Non-Coding Regions of LSC in Shaping Chloroplast Genome Size DOI Open Access
Huanchu Liu, Hans Jacquemyn, Yanlin Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3691 - 3691

Опубликована: Апрель 14, 2025

Cypripedium is renowned for its high morphological diversity and complex genetic evolutionary characteristics. The chloroplast genome serves as a valuable tool investigating phylogenetic relationships processes in plants. Currently, research on the evolution of within genus limited due to insufficient large-scale sampling lack comprehensive understanding. Consequently, mechanisms underlying significant differences size among species remain poorly understood. In this study, we conducted comparative analysis genomes 29 species. lengths these ranged from 162,092 246,177 base pairs (bp) contained between 127 134 genes. Our results indicate that, while overall structure relatively conserved, were observed large single-copy (LSC), small (SSC), inverted repeat (IR) regions. Several genes, including psaC, rpl32, ycf1, psbK, exhibited higher levels variability may serve molecular markers taxonomic studies. our correlation suggest that expansion LSC region, increase simple sequence repeats (SSRs), tandem (TRs) have significantly enlarged Phylogenetic signal testing supports notion variation has driven divergence genus. Overall, findings provide insights into substantial length However, relationship diversification affecting Cypripedium, ecological adaptive evolution, incomplete lineage sorting (ILS), hybridization, reticulate events, requires further investigation.

Язык: Английский

Процитировано

0
Phylogeny, character evolution and biogeography of Adonis (Ranunculaceae), with special emphasis on the out of Qinghai‐Tibet Plateau DOI
Yuan‐Yuan Ling, Kun‐Li Xiang, Huan‐Wen Peng

и другие.

Taxon, Год журнала: 2025, Номер unknown

Опубликована: Апрель 27, 2025

Abstract The genus Adonis (Ranunculaceae) contains about 30 species and is widespread in Eurasia. This some of pharmaceutical ornamental interest, yet a phylogenetic framework for understanding its evolution still lacking. It has been hypothesized that originated the Qinghai‐Tibet Plateau (QTP) migrated out QTP through three dispersal routes. biogeographical hypothesis never tested using molecular data. Using nine plastid nuclear DNA regions, we first reconstructed robust with currently recognized 31 species. We then inferred evolutionary patterns morphological characters, estimated divergence times, ancestral ranges. Our results show two subgenera A. subg. Adonanthe are monophyletic strong support. Among four non‐monotypic sections, only sect. as monophyletic. Habit, basal leaf, pollen, carpel carry signal , while achene, beak shape, length exhibit relatively higher levels flexibility. Oligocene (ca. Ma) subsequently dispersed into Irano‐Turanian region early Miocene 20 Ma). In out‐of‐QTP routes were from late to Pliocene. study provides comprehensive phylogeny highlights indispensable role uplift associated climatic events shaping current distribution endemism hence contributing knowledge on historical biogeography plants

Язык: Английский

Процитировано

0
Mitochondrial genome evolution in the orchid subfamily Cypripedioideae (Orchidaceae) DOI
Shiyu Qin,

Hanchen Wang,

Miaomiao Wang

и другие.

Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 30, 2025

Язык: Английский

Процитировано

0
Target Enrichment Data Uncovers Rapid Radiation, Whole Genome Duplication, and Extensive Hybridization in Slipper Orchid GenusCypripediumL. DOI Open Access

Loudmila Jelinscaia Lagou,

Gudrun Kadereit, Diego F. Morales‐Briones

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 27, 2024

Background and Aims Cypripedium is the most widespread morphologically diverse genus of slipper orchids. Despite several published phylogenies, topology monophyly its infrageneric taxa remained uncertain. Here, we aimed to reconstruct a robust section-level phylogeny explore evolutionary history using target capture data for first time. Methods We used orchid-specific bait set Orchidaceae963 in combination with transcriptomic based on 913 nuclear loci, covering all 13 sections. Subsequently, investigated discordance among chloroplast trees, estimated divergence times ancestral ranges, searched anomaly zones, polytomies, diversification rate shifts, identified potential gene (genome) duplication hybridization events. Key Results All sections were recovered as monophyletic, contrary two subsections within sect. . The subclades this section did not correspond but matched geographic distribution their species. Additionally, discovered high levels short backbone branches , which can be attributed events detected phylogenetic network analyses, incomplete lineage sorting caused by rapid radiation. Our biogeographic analysis suggested Neotropical origin during Oligocene (∼30 Ma), potentially hybrid spreading Old World Early Miocene (∼22 Ma). radiation at likely occurred Southeast Asia around Middle Climatic Transition (∼15–13 followed independent dispersals back New World. Moreover, Pliocene-Quaternary glacial cycles may have contributed further speciation reticulate evolution Conclusions study provided novel insights into high-throughput molecular data, shedding light dynamics diversity patterns from present.

Язык: Английский

Процитировано

1