Sertm2 is a conserved micropeptide that promotes GDNF-mediated motor neuron subtype specification

EMBO Reports, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Small open-reading frame-encoded micropeptides within long noncoding RNAs (lncRNAs) are often overlooked due to their small size and low abundance. However, emerging evidence links these various biological pathways, though roles in neural development neurodegeneration remain unclear. Here, we investigate the function of murine micropeptide Sertm2, encoded by lncRNA A730046J19Rik, during spinal motor neuron (MN) development. Sertm2 is predicted be a conserved transmembrane protein found both mouse human, with subcellular analysis revealing that it enriched cytoplasm neurites. By generating C terminally Flag-tagged expressing from A730046J19Rik locus, demonstrate localizes MNs mice. The GDNF signaling-induced Etv4+ pool impaired knockout mice, which display nerve arborization defects culminate coordination muscle weakness. Similarly, human SERTM2 iPSC-derived also reduced ETV4+ pools, highlighting novel, evolutionarily essential for maintaining GDNF-induced MN subtype identity.

Язык: Английский

---

Single-cell spatial transcriptomic atlas of the whole mouse brain

Neuron, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

---

An Inflammation Associated lncRNA Induces Neuronal Damage via Mitochondrial Dysfunction

Molecular Therapy — Nucleic Acids, Год журнала: 2025, Номер unknown, С. 102533 - 102533

Опубликована: Апрель 1, 2025

Immune disease-associated non-coding SNPs, which often locate in tissue-specific regulatory elements, are emerging as key factors gene regulation. Among these long RNAs (lncRNAs) participate many cellular processes, and their characteristics make molecules appealing therapeutic targets. In this study, we have studied lncRNA LOC339803 the context of neuronal cells, is located autoimmunity-associated region 2p15 recently described to a proinflammatory role intestinal disorders. Using human brain samples wide variety vitro techniques, showed differential function cells. We further demonstrated maintaining hexokinase 2 (HK2) levels thus mitochondrial integrity, partially explaining implication multiple sclerosis (MS) pathogenesis. Our results show importance cell-type-specific studies case lncRNAs. present candidate for dysfunction marker or possible target neurodegeneration.

Язык: Английский

---

A novel cancer-associated lncRNA, LINC01123, participates in tumor progression, metabolism, immune escape, and resistance

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 4, 2025

Long Intergenic Non-Protein Coding RNA 1123 (LINC01123), located on human chromosome 2q13, is a pivotal factor in tumorigenesis, exerting multifaceted oncogenic effects. Its expression strongly correlates with clinicopathological features, patient survival, and disease progression. In vivo vitro experiments further demonstrate that LINC01123 influences diverse cellular processes, including proliferation, apoptosis, viability, migration, invasion, stemness, tumor growth. Notably, it also regulates metabolic reprogramming, immune escape, cell resistance to treatment. regulated by multiple transcription factors participates gene regulation through protein interactions competitive endogenous (ceRNA) networks, thereby modulating cancer-promoting This work systematically elucidates its primary functions molecular mechanisms driving cancer initiation progression, suggesting might serve as novel potential driver biomarker various cancers.

Язык: Английский

---

Analgesic Mechanism of Emodin in Neuropathic Pain Through Inhibiting P2X4 Purinoceptor Signaling

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Язык: Английский

---