Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells DOI Creative Commons
Xi Yao, Min Liu, Pei Wang

и другие.

Archives of Oral Biology, Год журнала: 2025, Номер unknown, С. 106253 - 106253

Опубликована: Апрель 1, 2025

This study aimed to explore the impact of sitagliptin on inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) clarify underlying mechanisms action. In vitro-cultured BMSCs were identified, treated with a range doses, assessed cell counting kit-8 assay quantify viability. The expression proteins genes relevant inflammation osteogenesis was measured using enzyme-linked immunosorbent real-time quantitative polymerase chain reaction techniques. ability analyzed by alkaline phosphatase staining, activity assay, alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation detected through western blotting. High but not low concentrations significantly suppressed cellular Sitagliptin dose-dependently inhibited LPS-induced responses while facilitating their differentiation. It also activated AMPK suppressing NF-κB activity. inhibitor treatment partially reversed these beneficial effects osteogenesis. suppresses promoting modulation AMPK/NF-κB activity, thereby mitigating functional impairment under microenvironmental conditions.

Язык: Английский

Bone-protective effects of neutralizing angiopoietin-like protein 4 monoclonal antibody in rheumatoid arthritis DOI

Liqing Ke,

Qifei He,

Jing Qu

и другие.

Molecular Therapy, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

3
Exercise ameliorates osteopenia in mice via intestinal microbial-mediated bile acid metabolism pathway DOI Creative Commons
Congcong Yu,

Rongtai Sun,

Wentao Yang

и другие.

Theranostics, Год журнала: 2025, Номер 15(5), С. 1741 - 1759

Опубликована: Янв. 2, 2025

Rationale: Physical exercise is essential for skeletal integrity and bone health. The gut microbiome, as a pivotal modulator of overall physiologic states, closely associated with homeostasis metabolism. However, the potential role intestinal microbiota in exercise-mediated gain remains unclear. Methods: We conducted depletion fecal transplantation (FMT) ovariectomy (OVX) mice aged to investigate whether transfer ecological traits could confer exercise-induced protective effects. study analyzed metabolic profiles via 16S rRNA gene sequencing LC-MS untargeted metabolomics identify key microbial communities metabolites responsible protection. Transcriptome RNA interference were employed explore molecular mechanisms. Results: found that hindered osteogenic benefits exercise, FMT from exercised osteoporotic effectively mitigated osteopenia. Comprehensive profiling microbiome metabolome revealed exercise-matched reshaped microecology landscape. Notably, alterations bile acid metabolism, specifically enrichment taurine ursodeoxycholic acid, mediated effects on mass. Mechanistically, activated apelin signaling pathway restored bone-fat balance recipient MSCs. Conclusion: Our underscored important microbiota-metabolic axis gain, heralding breakthrough treatment osteoporosis.

Язык: Английский

Процитировано

0
Baicalein attenuates ovalbumin-induced allergic rhinitis through the activation of nuclear receptor subfamily 4 group a member 1 DOI
Ying Xu, Lili Xu, Jian Xiao

и другие.

Immunologic Research, Год журнала: 2025, Номер 73(1)

Опубликована: Янв. 14, 2025

Язык: Английский

Процитировано

0
Laboratory investigation of METTL7A driving MSC osteogenic differentiation through YAP1 translation enhancement via eIF4F recruitment DOI Open Access
Li Cui,

Zizhao Mai,

Ye Lu

и другие.

International Endodontic Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Effective control of mesenchymal stem cell (MSC) differentiation towards osteogenic lineages is fundamental for bone regeneration. This study elucidates the regulatory role methyltransferase like 7A (METTL7A) in MSCs. Alkaline phosphatase staining, Alizarin Red S western blotting, and vivo studies were conducted to determine effects METTL7A depletion or overexpression on various types Then downstream signalling pathways regulated by MSCs further investigated. Our findings indicate that expression significantly increases during Furthermore, hindered, whereas its enhanced, Mechanistically, influences MSC activating YAP1-TEAD1 pathway. It enhances YAP1 not only stabilising mRNA but also, crucially, recruiting eIF4F complex, thereby boosting translation efficiency mRNA. Additionally, YAP1/TEAD1 complex transcriptionally regulates expression, creating a positive feedback loop amplifies differentiation. Overall, our uncovers previously unknown molecular mechanism suggests could offer new avenues enhancing

Язык: Английский

Процитировано

0
Endothelial cell-modified BMSC-GT/PCL nanofiber membrane sheet constructs promote bone tissue regeneration DOI Creative Commons
Qian Zhou,

Mengnan Wen,

Yihang Zhang

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Фев. 28, 2025

Introduction Bone defect repair remains a major challenge in modern medicine. Although bone marrow mesenchymal stem cells (BMSCs) possess multilineage differentiation potential, traditional BMSC constructs are often limited clinical applications due to insufficient osteogenic efficiency and inadequate vascularization. Methods This study developed an innovative tissue engineering strategy by combining BMSCs with gelatin/polycaprolactone (GT/PCL) nanofiber membranes form cell sheets, which were then modified endothelial (ECs) on the surface. The sheets subsequently rolled into three-dimensional scaffolds systematically evaluate their potential underlying mechanisms. Resuilts Results showed that electrospun GT/PCL exhibited uniform fiber structure (diameter 200–500 nm), successfully mimicking microstructure of natural extracellular matrix. In vitro experiments demonstrated after 14 days culture, EC modification significantly enhanced compared unmodified controls, approximately 3-fold increase ALP expression (p < 0.05) 2.5-fold angiogenic factor VEGF 0.01). Subcutaneous implantation nude mice revealed superior formation capability EC-modified at both 4 8 weeks: micro-CT analysis density reaching 350 mg/cm 3 , surface area approaching 400 mm 2 volume fraction 20%, higher than control groups 0.0001). Immunohistochemical evaluation further confirmed more mature trabecular richer vascular networks groups. Discussion Mechanistic studies promoted regeneration through three key pathways: optimization local microenvironment for improved nutrient supply, activation intercellular synergistic signaling pathways, reconstruction physiological microenvironment. not only validates application value this composite but also provides important theoretical basis developing novel solutions.

Язык: Английский

Процитировано

0
Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells DOI Creative Commons
Xi Yao, Min Liu, Pei Wang

и другие.

Archives of Oral Biology, Год журнала: 2025, Номер unknown, С. 106253 - 106253

Опубликована: Апрель 1, 2025

This study aimed to explore the impact of sitagliptin on inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) clarify underlying mechanisms action. In vitro-cultured BMSCs were identified, treated with a range doses, assessed cell counting kit-8 assay quantify viability. The expression proteins genes relevant inflammation osteogenesis was measured using enzyme-linked immunosorbent real-time quantitative polymerase chain reaction techniques. ability analyzed by alkaline phosphatase staining, activity assay, alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation detected through western blotting. High but not low concentrations significantly suppressed cellular Sitagliptin dose-dependently inhibited LPS-induced responses while facilitating their differentiation. It also activated AMPK suppressing NF-κB activity. inhibitor treatment partially reversed these beneficial effects osteogenesis. suppresses promoting modulation AMPK/NF-κB activity, thereby mitigating functional impairment under microenvironmental conditions.

Язык: Английский

Процитировано

0