bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 21, 2024
Extracellular
vesicles
(EVs)
have
emerged
as
promising
tools
for
the
development
of
disease
biomarkers
and
therapeutics
because
they
can
transfer
various
bioactive
cargos
between
cells
in
vivo.
A
better
understanding
heterogeneous
properties
EVs
vivo
may
provide
insights
into
their
biological
roles
clinical
translation
potential.
As
a
proof-of-concept,
we
report
that
different
EV
subpopulations
from
skeletal
muscle
tissues
distinct
composition
signatures
diverse
effects
on
recipient
cells.
Multiple
cell
types
(e.g.,
myoblasts
endothelial
(ECs))
contribute
to
pool
tissue-derived
EVs,
large
(L-EVs)
are
enriched
with
proteins
related
metabolic
regulation,
whereas
small
(S-EVs)
original
cell-specific
function
regulation.
Compared
L-EVs,
S-EVs
exhibited
abundant
surface
higher
uptake
rates.
Moreover,
L-EVs
induce
changes
global
gene
expression,
patterns
some
cellular
behaviors
proliferation
differentiation)
These
results
suggest
might
control
tissue
hemostasis
coordinated
manner
importance
reconsidering
favorable
role
future
applications
biomarker
discovery
intervention).
This
study
highlights
functional
heterogeneity
vivo,
selection
an
ideal
subset
basis
its
specific
be
strategy
developing
more
precise
or
tailored
therapies
regenerative
medicine.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(9), С. 4144 - 4144
Опубликована: Апрель 27, 2025
Extrachromosomal
circular
DNAs
(eccDNAs)
are
heterogeneous
DNA
molecules
derived
from
genomic
DNA,
and
believed
to
be
involved
in
intercellular
communication
natural
biological
processes.
Extracellular
vesicles
(EVs)
membrane-bound
particles
released
all
cells,
have
been
shown
contain
various
classes
of
nucleic
acids.
EVs
can
play
a
role
may
used
as
biomarkers.
This
constitutes
the
first
study
demonstrate
that
healthy
human
dermal
fibroblasts
carry
eccDNA.
eccDNA
their
corresponding
donor
cells
were
isolated
sequenced
on
Oxford
Nanopore
MinIon
platform,
followed
by
identification
potential
eccDNAs
through
four
different
bioinformatic
pipelines,
namely
ecc_Finder,
cyrcular-calling,
CReSIL,
Flec.
Our
main
findings
eccDNA;
there
is
variability
number
identified
same
sample
pipelines;
across
replicates.
Additionally,
characterized
this
research
had
(a)
sequences
small
306
base
pairs
large
28,958
samples,
(b)
uneven
chromosomal
distribution,
(c)
an
average
49.7%
harboring
gene
fragments.
Future
implications
for
novel
include
using
framework
method
elucidate
factors
conditions
influence
skin
aging
process
related
biogenesis
cells.
Chemical Communications,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Extracellular
vesicles
have
been
deemed
as
potential
drug
carriers
for
treatment
of
various
diseases.
Recent
advances
summarized,
including
the
sources,
delivery
function,
extraction
and
cargo-loading
technology
extracellular
vesicles.
Abstract
High
myopia
stands
as
the
primary
cause
of
blindness
globally,
with
cataract
emerging
one
most
prevalent
complications.
However,
underlying
mechanism
high
myopic
remains
unknown.
The
lens
capsule
is
basement
membrane
enclosing
lens.
In
this
study,
we
hypothesized
tissue‐derived
extracellular
vesicles
(Ti‐EVs)
play
a
vital
role
in
formation
cataract.
Ti‐EVs
were
collected
from
and
age‐related
patients
during
surgery,
isolated
by
ExoDisc.
Then,
performed
proximity
barcoding
assay
for
single‐EV
analysis,
which
enabled
us
to
identify
alteration
Ti‐EV
subpopulations
associated
Among
detected
proteins,
our
findings
revealed
predominant
immunity
cluster
within
cataracts,
characterized
significantly
higher
abundance
macrophage‐derived
EVs
strongly
correlated
aquaporin
1
(AQP1)
cluster,
suggesting
potential
interaction
between
these
two
components
progression
It
was
also
observed
that
eye
morphogenesis
may
work
concert
AQP1,
potentially
driving
cataracts
through
pathway.
These
not
only
shed
new
light
on
mechanisms
cataract,
but
pave
way
development
novel
therapeutic
strategies
prevent
or
treat
devastating
condition.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 11, 2025
Summary
Extracellular
vesicles
(EVs)
mediate
cell-to-cell
communication
in
endocrine,
paracrine,
and
autocrine
fashions,
but
their
roles
transport
the
body
remain
incompletely
understood.
It
is
expected
that
EVs
circulate
through
bloodstream,
where
derived
from
various
tissues
are
present.
However,
how
much
each
tissue
contributes
to
circulating
largely
unknown.
Therefore,
identifying
tissue-specific
EV
markers
of
importance
for
elucidating
vivo
dynamics
EVs.
A
key
issue
hampers
studying
lack
methodologies
collecting
them
tissues.
Here,
we
developed
methods
isolate
four
different
tissues,
skeletal
muscle,
heart,
liver,
adipose
tissue,
performed
proteomic
analysis
on
Unbiased
quantitative
revealed
protein
signatures
identified
marker
proteins
specific
tissue.
Furthermore,
comprehensive
comparisons
proteome
plasma
EVs,
predicted
accessibility
circulation.
Our
data
suggests
that,
among
tissue-derived
more
readily
enter
circulation,
while
muscle-derived
exhibit
poor
blood.
Collectively,
our
circulation
highly
depends
original
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Март 17, 2025
Osteoarthritis
(OA)
is
the
most
common
degenerative
joint
disease
worldwide,
characterized
by
synovial
inflammation,
cartilage
loss,
and
reactive
hyperplasia
of
subchondral
bone,
affecting
quality
life
hundreds
millions
people.
However,
molecular
mechanisms
underlying
occurrence
progression
OA
remain
unclear,
there
no
therapy
can
substantially
interrupt
or
reverse
destructive
process
OA.
More
insight
into
pathogenesis
may
result
in
innovative
therapeutics.
The
microenvironment
plays
a
pivotal
role
development
OA,
which
encompasses
chondrocytes,
adipocytes,
fibroblasts,
endothelial
cells,
immune
cells.
Extracellular
vesicles
(EVs)
have
emerged
as
novel
form
intercellular
communication,
mediating
transfer
range
bioactive
molecules
to
create
specific
microenvironment.
Recent
studies
reported
that
cargos
EVs
play
crucial
including
noncoding
RNAs
(ncRNAs),
proteins,
lipids.
This
review
systematically
analyzes
summarizes
biological
characteristics
functionalities
derived
from
diverse
cellular
sources,
especially
how
mediate
communication
between
different
cells
microenvironment,
with
view
providing
new
insights
Abstract
Extracellular
vesicles
(EVs)
are
lipid
bilayer
structures
secreted
by
cells
that
act
as
intercellular
messengers.
Tissue‐derived
EVs
(TEVs),
harvested
from
the
tissue
interstitium,
directly
reflect
actual
physiological
or
pathological
state
of
microenvironment.
However,
difficulty
in
acquisition
severely
limits
development
TEVs.
In
contrast,
organoids
3D
cell
clusters
cultured
stem
cells,
which
have
spatial
and
functions
highly
similar
to
source
tissues.
Although
organoid‐derived
(OEVs),
isolated
culture
supernatants,
can
complex
cellular
interactions,
they
cannot
microenvironment
like
Building
on
foundation
TEVs
OEVs,
we
introduce
innovative
concept
organoid‐tissue
(OTEVs),
where
residing
organoid
interstitium.
Acting
a
communication
bridge
between
OEVs
TEVs,
OTEVs
accurately
represent
true
They
overcome
challenges
associated
with
limited
availability
inability
We
believe
will
synergize
enhance
understanding
pathogenesis
diseases,
well
improve
their
diagnosis
treatment.
