Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
13
Опубликована: Янв. 28, 2025
Introduction
Osteoarthritis
(OA)
is
a
degenerative
disease
of
the
joints
characterized
by
cartilage
degradation
and
synovial
inflammation.
Due
to
complex
pathogenesis
OA,
multifaceted
therapies
that
modulate
inflammatory
immune
microenvironmental
disturbances
while
promoting
regeneration
are
key
control
progression
OA.
Methods
Herein,
multifunctional
nanoparticle
(DIC/Mg-PDA
NPs)
was
constructed
successfully
metal
chelation
effect
between
Mg
2+
catecholamine
bond
from
dopamine,
followed
amidation
with
diclofenac
(DIC),
which
then
prepared
into
an
injectable
hydrogel
microsphere
(DIC/Mg-PDA@HM)
immune-regulating
cartilage-repairing
abilities
through
microfluidic
technology
for
treatment
osteoarthritis.
Results
discussion
The
sustained
release
composite
microspheres
achieved
regulation
converting
macrophages
M1
M2
promoted
differentiation
BMSCs.
Moreover,
enhanced
DIC
polydopamine
(PDA)
effectively
downregulated
factors,
finally
OA
therapy.
In
addition,
in
vivo
MRI
tissue
section
staining
model
proved
significant
efficacy
on
conclusion,
these
novel
demonstrated
promising
prospect
multidisciplinary
repairing
Язык: Английский
Protective Effects of Vitamin D on Proteoglycans of Human Articular Chondrocytes through TGF-β1 Signaling
Nutrients,
Год журнала:
2024,
Номер
16(17), С. 2991 - 2991
Опубликована: Сен. 4, 2024
The
extracellular
matrix
of
cartilage
primarily
constitutes
collagen
and
aggrecan.
Cartilage
degradation
starts
with
aggrecan
loss
in
osteoarthritis
(OA).
Vitamin
D
(VD)
plays
an
essential
role
several
inflammation-related
diseases
can
protect
the
during
OA.
present
study
focused
on
VD
turnover
human
articular
chondrocytes
treated
tumor
necrosis
factor
α
(TNF-α)
possible
mechanism.
Treatment
different
doses
periods
intervention
TNF-α
TGF-β1
receptor
(TGFβR1)
inhibitor
SB525334
were
investigated.
viability
secretion
measured.
expression
intracellular
TGFβR1
was
examined.
Transcriptional
translational
levels
related
metabolic
factors
analyzed.
results
showed
that
markedly
reduced
viability,
expressions
chondrocytes,
increased
disintegrin
metalloproteinase
thrombospondin
motifs.
alterations
partially
inhibited
by
treatment.
Furthermore,
effects
blocked
TNF-α-treated
cells.
may
prevent
proteoglycan
due
to
via
signaling
chondrocytes.
Язык: Английский