Automated assessment of the mouse body-language reveals pervasive behavioral disruption in a two-hit model of psychiatric vulnerability DOI Creative Commons

Viviana Canicatti,

Vanes Cibin,

Nicoletta Berardi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Abstract The influence of early-life experiences is widely acknowledged as a crafting tool that sculpts complex behavioral patterns and well-being living organisms. use preclinical models can provide invaluable insight into how negative environmental push interplays with genetic make-up in shaping psychiatric vulnerability. However, the assessment traits cross-species studies often relies on surrogate metrics proxy for internal state, limiting interpretation to context-dependent outcomes. In this work, we exploited validated computational digitalized ethological screening identify spontaneous hallmarks altered functioning dual-hit mouse model To do so, mice carrying heterozygous deletion gene coding Contactin-associated protein-like 2 ( Cntnap2 +/- ) their wild-type (WT) littermates were raised limited bedding nesting (LBN). These animals compared both WT standard conditions, mapping behavior during freely-moving exploration. Our data show descriptors motility state or anxiety indicators largely failed detecting subtle diversion from control conditions. By contrast, automated segmentation body-language revealed significant impact genotype experience programming. Thus, using unsupervised clustering, unveiled two alternative neurobehavioral profiles within our dataset. We found one identified overlapped LBN, while other was equally shared among controls. conclude coincidence adversity haploinsufficiency drastically reshapes structure rodents. SIGNIFICANCE STATEMENT Enhancing predictive face validity research remains challenge due inherent heterogeneity complexity these While animal are crucial understanding risk factors involved, replicating full conditions continues pose difficulties. study, investigate emotional double-hit (environmental genetic) vulnerability disorder, glimpsing subliminal disturbances not captured traditional assessments. findings highlight effectiveness novel tools identifying deviations support hypothesis gene-environment interaction contributes shape mice.

Язык: Английский

Automated assessment of the mouse body-language reveals pervasive behavioral disruption in a two-hit model of psychiatric vulnerability DOI Creative Commons

Viviana Canicatti,

Vanes Cibin,

Nicoletta Berardi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Abstract The influence of early-life experiences is widely acknowledged as a crafting tool that sculpts complex behavioral patterns and well-being living organisms. use preclinical models can provide invaluable insight into how negative environmental push interplays with genetic make-up in shaping psychiatric vulnerability. However, the assessment traits cross-species studies often relies on surrogate metrics proxy for internal state, limiting interpretation to context-dependent outcomes. In this work, we exploited validated computational digitalized ethological screening identify spontaneous hallmarks altered functioning dual-hit mouse model To do so, mice carrying heterozygous deletion gene coding Contactin-associated protein-like 2 ( Cntnap2 +/- ) their wild-type (WT) littermates were raised limited bedding nesting (LBN). These animals compared both WT standard conditions, mapping behavior during freely-moving exploration. Our data show descriptors motility state or anxiety indicators largely failed detecting subtle diversion from control conditions. By contrast, automated segmentation body-language revealed significant impact genotype experience programming. Thus, using unsupervised clustering, unveiled two alternative neurobehavioral profiles within our dataset. We found one identified overlapped LBN, while other was equally shared among controls. conclude coincidence adversity haploinsufficiency drastically reshapes structure rodents. SIGNIFICANCE STATEMENT Enhancing predictive face validity research remains challenge due inherent heterogeneity complexity these While animal are crucial understanding risk factors involved, replicating full conditions continues pose difficulties. study, investigate emotional double-hit (environmental genetic) vulnerability disorder, glimpsing subliminal disturbances not captured traditional assessments. findings highlight effectiveness novel tools identifying deviations support hypothesis gene-environment interaction contributes shape mice.

Язык: Английский

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