Virological characteristics of the SARS-CoV-2 XBB variant derived from recombination of two Omicron subvariants

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Май 16, 2023

In late 2022, SARS-CoV-2 Omicron subvariants have become highly diversified, and XBB is spreading rapidly around the world. Our phylogenetic analyses suggested that emerged through recombination of two cocirculating BA.2 lineages, BJ.1 BM.1.1.1 (a progeny BA.2.75), during summer 2022. XBB.1 variant most profoundly resistant to BA.2/5 breakthrough infection sera date more fusogenic than BA.2.75. The breakpoint located in receptor-binding domain spike, each region recombinant spike confers immune evasion increases fusogenicity. We further provide structural basis for interaction between human ACE2. Finally, intrinsic pathogenicity male hamsters comparable or even lower multiscale investigation provides evidence suggesting first observed increase its fitness rather substitutions.

Язык: Английский

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SARS-CoV-2 BA.2.86 enters lung cells and evades neutralizing antibodies with high efficiency

Cell, Год журнала: 2024, Номер 187(3), С. 596 - 608.e17

Опубликована: Янв. 8, 2024

Язык: Английский

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Profound neutralization evasion and augmented host cell entry are hallmarks of the fast-spreading SARS-CoV-2 lineage XBB.1.5

Cellular and Molecular Immunology, Год журнала: 2023, Номер 20(4), С. 419 - 422

Опубликована: Март 3, 2023

Since late 2022, the share of infections caused by SARS-CoV-2 lineage XBB.1.5has gradually increased in United States, resulting XBB.1.5becoming dominating States and a similar trend is likely to soon take place also European countries.However, information on virological properties XBB.1.5 scarce.Here, we conducted an initial assessment XBB.1.5lineage.The XBB possesses extraordinarily high capacity for antibody evasion due its unique set S protein mutations [1][2][3][4].However, this trait may have come at cost moderately reduced host cell entry efficiency as suggested recent vitro data [1,5], which explain why sublineages only accounted small proportion total several countries (except India) so far (Fig. 1a).Recently, has changed where sublineage since XBB.1.5now represents 1a).Moreover, although being presently detected low frequencies only, increase XBB.1.5-relatedinfections observed 1a).The XBB.1.5S differs one mutation (S486P) from parental XBB.1 lineage, located receptor-binding domain (RBD) 1b).Thus, it affect transmissibility modulating efficiency, alter sensitivity antibody-mediated neutralization.Here, performed XBB.1.5lineage neutralization, using protein-bearing pseudovirus particles (pp), are suitable model system analysis neutralization [6].Particles pseudotyped with ancestral B.1 (B.1 pp ) or Omicron BA.4/BA.5 (identical amino acid level, BA.4-5 ), BQ.1.1 (BQ.1.1 (XBB.1 were used comparison.First, compared line tropism different pseudoviruses.As expected, displayed augmented most lines tested exception

Язык: Английский

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Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency

Nature Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Июль 8, 2024

Язык: Английский

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Enabling the immune escaped etesevimab fully-armed against SARS-CoV-2 Omicron subvariants including KP.2

hLife, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(30)

Опубликована: Май 23, 2023

Mass pathogen screening is critical to preventing the outbreaks and spread of infectious diseases. The large-scale epidemic COVID-19 rapid mutation severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus have put forward new requirements for detection identification techniques. Here, we report a CRISPR-based Amplification-free Viral RNA Electrical Detection platform (CAVRED) SARS-CoV-2 variants. A series CRISPR assays were designed amplify CRISPR-Cas system's ability discriminate between mutant wild genomes with single-nucleotide difference. identified viral information was converted into readable electrical signals through field-effect transistor biosensors achievement highly sensitive single-base mutations. CAVRED can detect genome as low 1 cp μL-1 within 20 mins without amplification, this value comparable limit real-time quantitative polymerase chain reaction. Based on excellent ability, an 8-in-1 array constructed realized 40 simulated throat swab samples variants 95.0 % accuracy. advantages accuracy, sensitivity, fast speed promise its application in screening.

Язык: Английский

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Inactivated vaccine-elicited potent antibodies can broadly neutralize SARS-CoV-2 circulating variants

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Апрель 17, 2023

A full understanding of the inactivated COVID-19 vaccine-mediated antibody responses to SARS-CoV-2 circulating variants will inform vaccine effectiveness and vaccination development strategies. Here, we offer insights into vaccine-induced after prime-boost at both polyclonal monoclonal levels. We characterized VDJ sequence 118 antibodies (mAbs) found that 20 neutralizing mAbs showed varied potency breadth against a range including XBB.1.5, BQ.1.1, BN.1. Bispecific (bsAbs) based on nonoverlapping exhibited enhanced most antibody-evasive strains, such as The passive transfer or their bsAb effectively protected female hACE2 transgenic mice from challenge with an infectious Delta Omicron BA.2 variant. neutralization mechanisms these were determined by structural characterization. Overall, broad spectrum potent distinct can be induced in individuals immunized BBIBP-CorV, suggesting application potential vaccines for preventing infection variants.

Язык: Английский

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Neutralization of SARS-CoV-2 BQ.1.1, CH.1.1, and XBB.1.5 by breakthrough infection sera from previous and recent waves in China

Cell Discovery, Год журнала: 2023, Номер 9(1)

Опубликована: Июнь 27, 2023

Язык: Английский

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Neutralization against XBB.1 and XBB.1.5 after omicron subvariants breakthrough infection or reinfection

The Lancet Regional Health - Western Pacific, Год журнала: 2023, Номер 33, С. 100759 - 100759

Опубликована: Апрель 1, 2023

Dear editor, Currently, the omicron XBB subvariant, which is a recombinant of BJ.1 and BA.2.75, was first identified in India August 2022 now its new sublineage XBB.1.5, has been reported from at least 79 countries become predominant world,1WHOWeekly epidemiological update on COVID-19-8 March 2023.https://www.who.int/publications/m/item/weekly-epidemiological-update-on-covid-19---8-march-2023Date accessed: 15, 2023Google Scholar raising concern neutralizing activity elicited by breakthrough infection reinfection.2Kurhade C. Zou J. Xia H. et al.Low neutralization SARS-CoV-2 Omicron BA.2.75.2, BQ.1.1 XBB.1 parental mRNA vaccine or BA.5 bivalent booster.Nat Med. 2023; 29: 344-347Crossref PubMed Scopus (135) Google Scholar, 3Arora P. Cossmann A. Schulz S.R. al.Neutralisation sensitivity lineage.Lancet Infect Dis. 23: 147-148Summary Full Text PDF (17) 4Wang Q. Iketani S. Li Z. al.Alarming antibody evasion properties rising BQ subvariants.Cell. 186: 279-286.e8Summary (232) 5Yue Song W. Wang L. al.ACE2 binding enhanced transmissibility XBB.1.5.Lancet 278-280Summary (74) Therefore, we tested titers serum samples obtained participants six groups who suffered subvariants (BTI) reinfection against D614G strain primary BA.2, BA.4/5, BF.7, BQ.1.1, XBB.1, XBB.1.5 using pseudovirus test (pVNT) (Appendix pp 2, 8–13). In all groups, were lower than strain; level antibodies lowest followed (Fig. 1 Appendix p 4). For four months after BA.2.2 BTI, BF.7 that 1.8–4.0 times D614G, while 21.4–77.5 1A). 8 BTI without reinfection, 1.5–3.1 15.8–69.1 1B). The remained stable between 4 among subsequent BA.5.2 infection, no significant differences observed two time points 5–6). However, there also eight subjects 1.1–15.4 along with obvious reduction GMT ratios high percentage neutralized (87.5%) 1C). addition, significantly Further comparison showed BA.4/5 those stage 5–6), indicating previous more likely to reinfection. After BA.2.76 titer 2.6–6.3 relatively (37.6–91.9 times) 1D). 1.1–2.6 1.6–2.7 respectively. Differently, 16.4–60.3 17.0–94.9 respectively 1E F). higher variants group group. And resistant found compared 7). Our study one limitation. Due sample size small, factors sex, age, intervals symptoms onset sampling are mismatched different groups. studies large number needed analyse influence these future. Overall, our data suggest extensively escape We concluded correlated Continued assessment emerging needed. G.-L.W., L.-Q.F., Y.S. designed supervised research. L.-B.L., Y.S., H.-H.P., B.J., C.S., C.Q., W.-G.J. collected clinical data. J.-J.C., S.T., M.-C.L., T.-T.L. performed laboratory detection analyzed G.-L.W. L.-Q.F. drafted manuscript. All authors reviewed approved final declare competing interests. thank for providing study. This work supported Beijing Natural Science Foundation (L222119 Guo-Lin Wang), National Key Research Development Program China (2019YFC1200502 2019YFC1200604 Li-Qun Fang), (82103901 32000087 Bin Ji), Wuxi Medical Discipline (FZXK2021010 Yuan Shen), Project Technology (Basic Research) "Light Taihu Lake" (Y20212042 Chao Shi). funders had role design, collection, analysis, interpretation, writing report. Download .docx (1.32 MB) Help docx files

Язык: Английский

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A monoclonal antibody targeting a large surface of the receptor binding motif shows pan-neutralizing SARS-CoV-2 activity

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 5, 2024

Abstract Here we report the characterization of 17T2, a SARS-CoV-2 pan-neutralizing human monoclonal antibody isolated from COVID-19 convalescent individual infected during first pandemic wave. 17T2 is class 1 VH1-58/κ3-20 antibody, derived receptor binding domain (RBD)-specific IgA + memory B cell, with broad neutralizing activity against former and new variants, including XBB.1.16 BA.2.86 Omicron subvariants. Consistently, demonstrates in vivo prophylactic therapeutic BA.1.1 infection K18-hACE2 mice. Cryo-electron microscopy reconstruction shows that binds BA.1 spike RBD “up” position blocks motif, as other structurally similar antibodies do, S2E12. Yet, unlike S2E12, retains its all variants tested, probably due to larger contact area. These results highlight impact small structural changes on performance identify potential candidate for future clinical interventions.

Язык: Английский

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