Selecting Competent Reverse Transcription Strategies to Maximise Biodiversity Recovery With eRNA Metabarcoding

Molecular Ecology Resources, Год журнала: 2025, Номер unknown

Опубликована: Фев. 22, 2025

ABSTRACT Both environmental DNA (eDNA) and RNA (eRNA) have been widely adopted for biodiversity assessment. While eDNA often persists longer in environments, eRNA offers a more current view of biological activities. In metabarcoding, extracted is reverse transcribed into complementary (cDNA) metabarcoding. However, the efficacy various transcription strategies has not evaluated. Here we compared recovery efficiency three strategies: random priming with hexamers, oligo(dT) taxa‐specific using Mifish‐U fish both high‐ low‐biodiversity regions. Our results demonstrate that significantly impact recovery. Random consistently detected highest number taxa low‐ high‐biodiversity areas, performed comparably to hexamers; however, regions, hexamers outperformed oligo(dT), particularly recovering rare taxa. was comparable other high‐abundance taxa, it less effective thus limiting its utility comprehensive These differences are largely due multiple binding sites fewer or absent primers under high degradation. Combining improved recovery, especially low‐abundance species, supporting best practice eukaryotes. For prokaryotes genes lacking polyadenylation, favoured over taxa‐ gene‐specific priming. Collectively, these findings underscore critical importance selecting appropriate significant implications monitoring conservation efforts.

Язык: Английский

---

Exploring technical improvements for environmental nucleic acids-based biodiversity assessment and management in coastal ecosystems

Journal of Environmental Management, Год журнала: 2025, Номер 377, С. 124724 - 124724

Опубликована: Фев. 27, 2025

Язык: Английский

---

Asymmetric Effect of Natural Resource Exploitation on Climate Change in Resource-Rich African Countries

Standards, Год журнала: 2025, Номер 5(1), С. 7 - 7

Опубликована: Фев. 28, 2025

This study investigated the asymmetric impact of natural resource exploitation on climate change in resource-rich African countries, based panel data from 1980 to 2022. The dynamic common correlated effect (DCCE) and seemingly unrelated regression (DSUR) econometric techniques were employed evaluate long-term effects positive shocks negative exploitation. findings revealed a relationship between both temperature, with increases exerting more intensified temperature than decreases. In contrast, changes are negatively related precipitation, an increased intensity having pronounced rainfall patterns. also highlights critical role control variables such as GDP per capita, urban population, total energy consumption altering precipitation underscore importance adopting sustainable extraction practices, integrating green technologies, promoting collaboration across renewable value chains mitigate impacts

Язык: Английский

---

Applying the CORVI tool for designing localized Climate Risk Management: A case study of Dagupan City

Ocean & Coastal Management, Год журнала: 2025, Номер 263, С. 107602 - 107602

Опубликована: Март 1, 2025

Язык: Английский

---

Submerged and Emerged Rigid Vegetation Impact on Bedforms and Sediment Suspension under Wave Action

Coastal Engineering, Год журнала: 2025, Номер unknown, С. 104739 - 104739

Опубликована: Март 1, 2025

Язык: Английский

---