Quantitative Super‐Resolution Imaging of Molecular Tension DOI Creative Commons
Seong Ho Kim, Adam B. Yasunaga,

Hongyuan Zhang

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Abstract DNA‐based molecular tension probes have revolutionized the localization of mechanical events in live cells with super‐resolution. However, imaging magnitude these forces at super‐resolution has been challenging. Here, qtPAINT (quantitative points accumulation for nanoscale topography) is introduced as a strategy to image accuracy. By leveraging force‐dependent dissociation kinetics short DNA oligonucleotides on their complementary strands, encoded individual molecules through binding kinetics. This method allowed quantitative analysis kinetics, providing detailed reconstruction force magnitudes acting each probe. The technique integrates molecular‐beacon PAINT imager hairpin probe, achieving quantification range 9–30 pN and maintaining spatial resolution 30–120 nm low high‐density regions. Additionally, offers temporal order minute, enhancing its applicability studying dynamic cellular processes.

Язык: Английский

Integrin force loading rate in mechanobiology: from model to molecular measurement DOI Creative Commons
Hongyuan Zhang, Micah Yang, Seong Ho Kim

и другие.

QRB Discovery, Год журнала: 2025, Номер 6

Опубликована: Янв. 1, 2025

Abstract Integrins are critical transmembrane receptors that connect the extracellular matrix (ECM) to intracellular cytoskeleton, playing a central role in mechanotransduction – process by which cells convert mechanical stimuli into biochemical signals. The dynamic assembly and disassembly of integrin-mediated adhesions enable adapt continuously changing cues, regulating essential processes such as adhesion, migration, proliferation. In this review, we explore molecular clutch model framework for understanding dynamics integrin ECM interactions, emphasizing importance force loading rate. We discuss how rate bridges internal actomyosin-generated forces properties like stiffness ligand density, determining whether sufficient is transmitted mechanosensitive proteins talin. This transmission leads talin unfolding activation downstream signalling pathways, ultimately influencing cellular responses. also examine recent advances single-molecule DNA tension sensors have enabled direct measurements rates, refining range approximately 0.5–4 pN/s. These findings deepen our force-mediated underscore need improved sensor designs overcome current limitations.

Язык: Английский

Процитировано

1
Quantitative Super‐Resolution Imaging of Molecular Tension DOI Creative Commons
Seong Ho Kim, Adam B. Yasunaga,

Hongyuan Zhang

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Abstract DNA‐based molecular tension probes have revolutionized the localization of mechanical events in live cells with super‐resolution. However, imaging magnitude these forces at super‐resolution has been challenging. Here, qtPAINT (quantitative points accumulation for nanoscale topography) is introduced as a strategy to image accuracy. By leveraging force‐dependent dissociation kinetics short DNA oligonucleotides on their complementary strands, encoded individual molecules through binding kinetics. This method allowed quantitative analysis kinetics, providing detailed reconstruction force magnitudes acting each probe. The technique integrates molecular‐beacon PAINT imager hairpin probe, achieving quantification range 9–30 pN and maintaining spatial resolution 30–120 nm low high‐density regions. Additionally, offers temporal order minute, enhancing its applicability studying dynamic cellular processes.

Язык: Английский

Процитировано

0