Functional redundancy in the toxic pathway of Bt protein Cry1Ab, but not Cry1Fa, against the Asian corn borer DOI Creative Commons
Xingliang Wang,

Ya-Jun Yue,

Yuqian Zhai

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(16)

Опубликована: Апрель 17, 2025

Crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have been used extensively control some major crop pests, but their benefits decrease when pests evolve resistance. Better understanding of genetic basis resistance is needed effectively monitor, manage, and counter pest Bt crops. Resistance in at least 11 species Lepidoptera, including many important associated with naturally occurring mutations that disrupt one or more three larval midgut proteins: cadherin ATP-binding cassette ABCC2 ABCC3. Here, we determined how CRISPR/Cas9-mediated disrupting cadherin, ABCC2, ABCC3 singly pairs affect Cry1Ab Cry1Fa Asian corn borer ( Ostrinia furnacalis ), which most damaging Asia closely related European nubilalis a Europe North America. The results bioassays six knockout strains parent susceptible strain support model can kill larvae via path requiring another ABCC3, whereas uses only first path. model’s predictions are generally supported by linkage analyses responses Sf9 cells Xenopus oocytes modified pairs. functional redundancy identified here for could sustain its efficacy against O. may exemplify widespread natural strategy delaying

Язык: Английский

Functional redundancy in the toxic pathway of Bt protein Cry1Ab, but not Cry1Fa, against the Asian corn borer DOI Creative Commons
Xingliang Wang,

Ya-Jun Yue,

Yuqian Zhai

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(16)

Опубликована: Апрель 17, 2025

Crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) have been used extensively control some major crop pests, but their benefits decrease when pests evolve resistance. Better understanding of genetic basis resistance is needed effectively monitor, manage, and counter pest Bt crops. Resistance in at least 11 species Lepidoptera, including many important associated with naturally occurring mutations that disrupt one or more three larval midgut proteins: cadherin ATP-binding cassette ABCC2 ABCC3. Here, we determined how CRISPR/Cas9-mediated disrupting cadherin, ABCC2, ABCC3 singly pairs affect Cry1Ab Cry1Fa Asian corn borer ( Ostrinia furnacalis ), which most damaging Asia closely related European nubilalis a Europe North America. The results bioassays six knockout strains parent susceptible strain support model can kill larvae via path requiring another ABCC3, whereas uses only first path. model’s predictions are generally supported by linkage analyses responses Sf9 cells Xenopus oocytes modified pairs. functional redundancy identified here for could sustain its efficacy against O. may exemplify widespread natural strategy delaying

Язык: Английский

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