Photoinduced Electrochemiluminescence Immunoassays DOI
Dongni Han, Jasmina Vidić, Dechen Jiang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(45), С. 18262 - 18268

Опубликована: Ноя. 1, 2024

Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge the improvement operation conditions required bead-based using typical [Ru(bpy)3]2+/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates imaging and quantitative analysis [Ru(bpy)3]2+-modified immunobeads at low anodic potential. The photovoltage generated by photoanode under light promotes oxidation processes electrode/electrolyte interface, thus considerably lowering onset potential both TPrA ECL emission. anti-Stokes shift between excitation (invisible to human eyes) visible emitted results in clear stable signal from immunobeads. addition, it offers possibility site-selective photoexcitation process. This approach not only meets performance traditional accuracy but also additional benefits lower requirements enhanced stability, providing new perspective optimization commercial immunoassays.

Язык: Английский

Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence DOI Creative Commons

Vanshika Gupta,

Francesco Falciani, Brady R. Layman

и другие.

Chemical & Biomedical Imaging, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0
Real-Time Visualization of an Elusive, Strong Reducing Agent during Tris(2,2'-bipyridyl)ruthenium(II) Electro-Oxidation in Water DOI
Megan L. Hill, Brady R. Layman, Jeffrey E. Dick

и другие.

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Май 16, 2025

Recently, liquid|liquid and liquid|gas interfaces have been implicated in driving unexpected chemistries, including dramatic rate enhancement spontaneous redox reactions. Given such studies, new methods are necessary to observe implicate reactive species. Tris(2,2'-bipyridyl)ruthenium(II) ([Ru(bpy)3]2+) is a common luminophore for photoluminescence electrochemiluminescence (ECL) studies. In this work, we demonstrate that the electro-oxidation of [Ru(bpy)3]2+ water produces light without addition sacrificial coreactants. We studied by confining an aqueous droplet adhered both tin-doped indium oxide electrode and, separately, glassy carbon inlaid disc macroelectrode (d = 3 mm). also generalized method observation at larger electrodes. The intensity higher absence O2, diminishes when adding H2O2, disappears presence well-behaved, one-electron oxidant (hexaammineruthenium(III)). Our results indicate powerful reducing agent present during [Ru(bpy)3]2+. This least energetic enough create excited state, [Ru(bpy)3]2+*, giving minimum energy ∼2 eV. Chemiluminescence persists as [Ru(bpy)3]3+ diffuses into solution, indicating strong may exist natively low abundance. These observations significant fundamental ramifications because they elucidate pathway ECL allow real-time visualization highly

Язык: Английский

Процитировано

0
Dual-Channel electrochemiluminescence mechanism and bioanalysis of semiconductor solar cell material Cu2ZnSnS4 DOI
Yan Liu, Xue Han,

Guorong Huang

и другие.

Journal of Electroanalytical Chemistry, Год журнала: 2025, Номер unknown, С. 119207 - 119207

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Electrochemiluminescence of N,N′-Dimethylformamide Passivated Black Phosphorus Quantum Dots DOI
Shuijian He, Weihan Li, Minsi Li

и другие.

Langmuir, Год журнала: 2024, Номер unknown

Опубликована: Дек. 19, 2024

Black phosphorus quantum dots (BPQDs) have shown promising applications in biosensors and energy storage devices. However, the electrochemiluminescence (ECL) properties of pristine BPQDs an organic system rarely been reported. In this paper, N,N′-dimethylformamide passivated with a small size 2.3 nm were obtained by ultrasonication-assisted liquid exfoliation process, their ECL studied. A reversible reduction peak was recorded differential pulse voltammetry, while no apparent oxidation observed. signal not seen annihilation route. Persulfate proved to be effective coreactant yellow emission observed which greatly red-shifted comparison that photoluminescence. is believed generated from both surface states electron promotion over band gap.

Язык: Английский

Процитировано

3
Photoinduced Electrochemiluminescence Immunoassays DOI
Dongni Han, Jasmina Vidić, Dechen Jiang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(45), С. 18262 - 18268

Опубликована: Ноя. 1, 2024

Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge the improvement operation conditions required bead-based using typical [Ru(bpy)3]2+/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates imaging and quantitative analysis [Ru(bpy)3]2+-modified immunobeads at low anodic potential. The photovoltage generated by photoanode under light promotes oxidation processes electrode/electrolyte interface, thus considerably lowering onset potential both TPrA ECL emission. anti-Stokes shift between excitation (invisible to human eyes) visible emitted results in clear stable signal from immunobeads. addition, it offers possibility site-selective photoexcitation process. This approach not only meets performance traditional accuracy but also additional benefits lower requirements enhanced stability, providing new perspective optimization commercial immunoassays.

Язык: Английский

Процитировано

2