Noncanonical Amino Acids Dictate Peptide Assembly in Living Cells
Accounts of Chemical Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
ConspectusEmulating
the
structural
features
or
functions
of
natural
systems
has
been
demonstrated
as
a
state-of-the-art
strategy
to
create
artificial
functional
materials.
Inspired
by
assembly
and
bioactivity
proteins,
self-assembly
peptides
into
nanostructures
represents
promising
approach
for
creating
biomaterials.
Conventional
assembled
peptide
biomaterials
are
typically
formulated
in
solution
delivered
pathological
sites
implementing
theranostic
objectives.
However,
this
translocation
entails
switch
from
formulation
conditions
physiological
environment
raises
concerns
about
material
performance.
In
addition,
precise
efficient
accumulation
administered
at
target
remains
significant
challenge,
leading
potential
biosafety
issues
associated
with
off-target
effects.
These
limitations
significantly
hinder
progress
advanced
To
address
these
concerns,
past
few
years
have
witnessed
development
situ
living
new
endeavor
optimizing
biomaterial
performance
benefiting
advances
stimuli-responsive
reactions
regulating
noncovalent
interactions.
refers
processes
via
sites.
Due
advantages
precisely
forming
well-defined
lesions,
situ-formed
assemblies
integrated
interesting
next-generation
biomedical
agents.Despite
great
developing
agents,
research
area
still
suffers
limited
toolkit
operating
under
complicated
conditions.
Considering
amino
acids
being
incorporated
backbones
modified
units,
an
acid
is
concern.
Therefore,
our
laboratory
intensively
engaged
designing
discovering
noncanonical
(ncAAs)
expand
manipulating
various
biological
Thus
far,
we
synthesized
containing
ncAAs
4-aminoproline,
2-nitroimidazole
alanine,
Se-methionine,
sulfated
tyrosine,
glycosylated
serine,
which
allow
us
develop
acid-responsive,
redox-responsive,
enzyme-responsive
systems.
Based
on
ncAAs,
established
complex
self-sorting
assembly,
self-amplified
dissipative
cells
optimize
peptides.
The
resulting
exhibit
morphological
adaptability
microenvironment,
contributes
overcoming
delivery
barriers
improvement
targeting
accumulation.
utilizing
developed
toolkit,
further
created
supramolecular
PROTACs,
antagonists,
probes
cancer
treatment
diagnosis
highlight
implications
usage.
Account,
summarize
journey
emphasis
mechanism
Eventually,
also
provide
forward
conceiving
prospects
challenges
clinical
translation
situ-formulated
Язык: Английский
DNA Nanostructures for Rational Regulation of Cellular Organelles
JACS Au,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
DNA
nanotechnology
has
revolutionized
materials
science
and
biomedicine
by
enabling
precise
manipulation
of
matter
at
the
nanoscale.
nanostructures
(DNs)
in
particular
represent
a
promising
frontier
for
targeted
therapeutics.
Engineered
DNs
offer
unprecedented
molecular
programmability,
biocompatibility,
structural
versatility,
making
them
ideal
candidates
advanced
drug
delivery,
organelle
regulation,
cellular
function
modulation.
This
Perspective
explores
emerging
role
modulating
behavior
through
organelle-targeted
interventions.
We
highlight
current
advances
nuclear,
mitochondrial,
lysosomal
targeting,
showcasing
applications
ranging
from
gene
delivery
to
cancer
For
instance,
have
enabled
precision
mitochondrial
disruption
cells,
pH
modulation
enhance
silencing,
nuclear
gene-editing
templates.
While
hold
immense
promise
advancing
nanomedicine,
outstanding
challenges
include
optimizing
biological
interactions
addressing
safety
concerns.
highlights
potential
rational
control
organelles,
which
could
lead
novel
therapeutic
strategies
advancement
nanomedicines
future.
Язык: Английский
Functionalization of Nucleic Acid Molecular Machines under Physiological Conditions: A Review
ACS Applied Bio Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
In-situ
fabrication
of
nucleic
acid
molecular
machines
in
biological
environments
is
desirable
for
smart
theranostic
applications.
However,
given
the
complex
nature
environments,
integration
multiple
functional
modules
into
a
coordinated
machine
remains
challenging.
Recent
advances
nanotechnology
offer
solutions
to
these
challenges.
Here,
we
outline
design
principles
acid–based
tailored
physiological
conditions,
drawing
on
recent
examples.
We
review
cutting-edge
technologies
that
facilitate
their
functionalization
settings,
particularly
presynthesis
modifications
using
unnatural
bases
and
postsynthesis
via
bioorthogonal
chemistry
noncovalent
interactions.
discuss
advantages
limitations
suggest
future
directions
overcome
existing
Язык: Английский
Synthetic Nanoassemblies for Regulating Organelles: From Molecular Design to Precision Therapeutics
ACS Nano,
Год журнала:
2024,
Номер
18(44), С. 30224 - 30246
Опубликована: Окт. 23, 2024
Each
organelle
referring
to
a
complex
multiorder
architecture
executes
respective
biological
processes
via
its
distinct
spatial
organization
and
internal
microenvironment.
As
the
assembly
of
biomolecules
is
structural
basis
living
cells,
creating
synthetic
nanoassemblies
with
specific
physicochemical
morphological
properties
in
cells
interfere
or
couple
natural
architectures
has
attracted
great
attention
precision
therapeutics
cancers.
In
this
review,
we
give
an
overview
latest
advances
for
precise
regulation,
including
formation
mechanisms,
triggering
strategies,
biomedical
applications
therapeutics.
We
summarize
emerging
material
systems,
polymers,
peptides,
deoxyribonucleic
acids
(DNAs),
their
intermolecular
interactions
intercellular
nanoassemblies,
highlight
design
principles
constructing
precursors
that
assemble
into
targeting
organelles
cellular
environment.
further
showcase
developed
intracellular
mitochondria,
endoplasmic
reticulum,
lysosome,
Golgi
apparatus,
nucleus
describe
underlying
mechanisms
regulation
cancer.
Last,
essential
challenges
field
prospects
future
are
discussed.
This
review
should
facilitate
rational
organelle-targeting
comprehensive
recognition
by
materials
contribute
deep
understanding
application
Язык: Английский
Metal Ion-Condensed DNA Nanoparticle Library: Phase Separation and Transition and Antisense Therapy Applications
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(43), С. 59116 - 59127
Опубликована: Окт. 20, 2024
DNA
condensation
has
long
been
investigated
as
a
fundamental
cellular
activity
and
is
known
to
be
driven
by
the
mediation
of
diverse
condensing
agents.
The
phase
behaviors
during
are
particularly
interesting
because
complicated
molecular
structure
natural
nucleotides
fundamentally
allows
electrostatic,
coordinate
covalent,
various
other
secondary
interactions
with
Recently,
metal
ion
(M
Язык: Английский