Extracellular vesicles as a drug delivery system: A systematic review of preclinical studies

Advanced Drug Delivery Reviews, Год журнала: 2021, Номер 175, С. 113801 - 113801

Опубликована: Май 18, 2021

Язык: Английский

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Functional siRNA Delivery by Extracellular Vesicle–Liposome Hybrid Nanoparticles

Advanced Healthcare Materials, Год журнала: 2021, Номер 11(5)

Опубликована: Авг. 11, 2021

The therapeutic use of RNA interference is limited by the inability siRNA molecules to reach their site action, cytosol target cells. Lipid nanoparticles, including liposomes, are commonly employed as carrier systems overcome this hurdle, although widespread remains due a lack delivery efficiency. More recently, nature's own carriers RNA, extracellular vesicles (EVs), increasingly being considered alternative vehicles intrinsic properties. However, they difficult load with exogenous cargo. Here, EV-liposome hybrid nanoparticles (hybrids) prepared and evaluated an system combining properties both liposomes EVs. It shown that hybrids spherical particles encapsulating siRNA, contain EV-surface makers, functionally deliver different cell types. functional behavior hybrids, in terms cellular uptake, toxicity, gene-silencing efficacy, altered compared varies among recipient Moreover, produced cardiac progenitor (CPC) derived-EVs retain attributed CPC-EVs such activation endothelial signaling migration. To conclude, combine benefits synthetic biological drug might serve future siRNA.

Язык: Английский

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Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy

Nature Biomedical Engineering, Год журнала: 2023, Номер 7(7), С. 887 - 900

Опубликована: Янв. 12, 2023

Язык: Английский

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Enzyme Therapy: Current Challenges and Future Perspectives

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(17), С. 9181 - 9181

Опубликована: Авг. 25, 2021

In recent years, enzymes have risen as promising therapeutic tools for different pathologies, from metabolic deficiencies, such fibrosis conditions, ocular pathologies or joint problems, to cancer cardiovascular diseases. Treatments based on the catalytic activity of are able convert a wide range target molecules restore correct physiological metabolism. These treatments present several advantages compared established approaches thanks their affinity and specificity properties. However, some challenges, short in vivo half-life, lack targeted action and, particular, patient immune system reaction against enzyme. For this reason, it is important monitor serum response during treatment. This can be achieved by conventional techniques (ELISA) but also new microarrays. assays gained popularity due high-throughput analysis capacity, simplicity, potential patients enzyme therapies. growing field, research still ongoing solve current health problems COVID-19. Currently, alternatives using angiotensin-converting 2 (ACE2) being studied treat

Язык: Английский

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Microvesicles transfer mitochondria and increase mitochondrial function in brain endothelial cells

Journal of Controlled Release, Год журнала: 2021, Номер 338, С. 505 - 526

Опубликована: Авг. 24, 2021

Язык: Английский

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Overview and Update on Methods for Cargo Loading into Extracellular Vesicles

Processes, Год журнала: 2021, Номер 9(2), С. 356 - 356

Опубликована: Фев. 15, 2021

The enormous library of pharmaceutical compounds presents endless research avenues. However, several factors limit the therapeutic potential these drugs, such as drug resistance, stability, off-target toxicity, and inadequate delivery to site action. Extracellular vesicles (EVs) are lipid bilayer-delimited particles naturally released from cells. Growing evidence shows that EVs have great serve effective carriers. Since can not only transfer biological information, but also effectively deliver hydrophobic drugs into cells, application a novel system has attracted considerable scientific interest. Recently, loaded with siRNA, miRNA, mRNA, CRISPR/Cas9, proteins, or show improved efficiency effect. In this review, we summarize methods used for cargo loading EVs, including drugs. Furthermore, include recent advance in engineered delivery. Finally, both advantages challenges new discussed. Here, encourage researchers further develop convenient reliable clinical applications carriers future.

Язык: Английский

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Pharmacokinetics and biodistribution of extracellular vesicles administered intravenously and intranasally to Macaca nemestrina

Journal of Extracellular Biology, Год журнала: 2022, Номер 1(10)

Опубликована: Окт. 1, 2022

Abstract Extracellular vesicles (EVs) have potential in disease treatment since they can be loaded with therapeutic molecules and engineered for retention by specific tissues. However, questions remain on optimal dosing, administration pharmacokinetics. Previous studies addressed biodistribution pharmacokinetics rodents, but little evidence is available larger animals. Here, we investigated the of Expi293F‐derived EVs labelled a highly sensitive nanoluciferase reporter (palmGRET) non‐human primate model ( Macaca nemestrina ), comparing intravenous (IV) intranasal (IN) over 125‐fold dose range. We report that administered IV had longer circulation times plasma than previously reported mice were detectable cerebrospinal fluid after 30–60 min. EV association peripheral blood mononuclear cells, especially B‐cells, was observed as early 1‐min post‐administration. detected liver spleen within 1 h administration. IN delivery minimal, suggesting pretreatment approaches may needed large Furthermore, strongly decreased repeated administration, possibly due to immune responses clear implications xenogeneic EV‐based therapeutics. hope our findings from this baseline study macaques will help inform future research development EVs.

Язык: Английский

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Exosome nanovesicles: A potential carrier for therapeutic delivery

Nano Today, Год журнала: 2023, Номер 49, С. 101771 - 101771

Опубликована: Фев. 7, 2023

Exosomes are small nanosized biovesicles that form when multivesicular bodies and the plasma membrane fuse released into surrounding body fluids. They best known for their multifunction in mediating intercellular communication by transferring various biomolecules, including DNA, RNAs, proteins, lipids, a short- long-distance manner have been identified as health disease messengers. Importantly, exosomes necessary physiological processes disease. The generation of depends on status disease, which usually exhibits opposite roles inducing enhanced cellular stress damage. Recently, exosome-based nanotechnologies provided unprecedented opportunities to boost developments exosome-related biology, chemistry, pathology, therapeutics different diseases based unique structural/compositional/morphological characteristics next-generation nanomedicines. Herein, we provide comprehensive overview recent advances exosome nanotechnology research, classification, isolation preparation, constitution, biological function, nanobiomedical applications treatment diagnosis. Furthermore, future prospects were also concluded. This review will more inspiration promoting development advanced theranostic nanoplatforms nanotechnology.

Язык: Английский

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Scalable Production of Extracellular Vesicles and Its Therapeutic Values: A Review

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(14), С. 7986 - 7986

Опубликована: Июль 20, 2022

Extracellular vesicles (EVs) are minute with lipid bilayer membranes. EVs secreted by cells for intercellular communication. Recently, have received much attention, as they rich in biological components such nucleic acids, lipids, and proteins that play essential roles tissue regeneration disease modification. In addition, can be developed vaccines against cancer infectious diseases, the vesicle membrane has an abundance of antigenic determinants virulent factors. therapeutic applications typically collected from conditioned media cultured cells. However, number is limited. Thus, it critical to devise new strategies large-scale production EVs. Here, we discussed utilized researchers scalable Techniques bioreactors, mechanical stimulation, electrical thermal magnetic field topographic clue, hypoxia, serum deprivation, pH modification, exposure small molecules, nanoparticles, increasing intracellular calcium concentration, genetic modification been used improve secretion nitrogen cavitation, porous extrusion, sonication prepare EV-mimetic nanovesicles share many characteristics naturally Apart inducing EV production, these upscaling interventions also reported modify EVs' cargo thus their functionality potential. summary, imperative identify a reliable technique produce large quantities consistently. Ideally, produced should possess improved

Язык: Английский

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Apoptotic bodies for advanced drug delivery and therapy

Journal of Controlled Release, Год журнала: 2022, Номер 351, С. 394 - 406

Опубликована: Сен. 27, 2022

Язык: Английский

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