Analytical Chemistry,
Год журнала:
2024,
Номер
96(43), С. 17154 - 17164
Опубликована: Окт. 17, 2024
Real-time
monitoring
of
chemotherapy-induced
senescence
(CIS)
in
cancer
remains
a
challenging
task
that
would
lead
to
new
insights
into
the
adaptive
mechanisms
therapy
and
provide
guidance
for
management.
Here,
we
designed
tailor-made
nanoprobe
capable
imaging
CIS
sequential
activation
self-amplified
manner
by
reversing
senescence-related
impaired
ferritinophagy.
It
contains
an
amphipathic
polymer
as
spatially
responsive
vehicle,
Fe2+-activable
dye
reporter,
autophagy
inducer
signal
amplifier.
Owing
metabolic
changes,
preferentially
enriches
senescent
cells,
leading
situ
fluorescence
switching
reporter
labile
Fe2+.
Meanwhile,
restores
ferritinophagy
promotes
autophagic
degradation
accumulated
ferritin,
facilitating
conversion
ferritin-bound
iron
Fe2+
amplified
cells
yet
keeping
inert
nonsenescent
cells.
Of
note,
accumulation
sustained
ferritin
occur
at
same
subcellular
location,
thus
minimizing
diffusion
process-induced
nonspecific
responses.
The
feasibility
this
strategy
is
successfully
demonstrated
both
living
animal
models.
This
work
offers
way
therapeutic
evaluation
basic
understanding
roles
treatment.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(32)
Опубликована: Май 23, 2024
Clear
delineation
of
tumor
margins
is
essential
for
accurate
resection
and
decreased
recurrence
rate
in
the
clinic.
Fluorescence
imaging
emerging
as
a
promising
alternative
to
traditional
visual
inspection
by
surgeons
intraoperative
imaging.
However,
probes
lack
accuracy
diagnosis,
making
it
difficult
depict
boundaries
accurately.
Herein,
we
proposed
an
offensive
defensive
integration
(ODI)
strategy
based
on
"attack
systems
(invasive
peptidase)
defense
(reductive
microenvironment)"
multi-dimensional
characteristics
design
activatable
fluorescent
precisely.
Screened
out
from
series
ODI
strategy-based
probes,
ANQ
performed
better
than
unilateral
correlation
distinguishing
between
cells
normal
minimizing
false-positive
signals
living
metabolic
organs.
To
further
improve
signal-to-background
ratio
vivo,
derivatized
FANQ,
was
prepared
successfully
applied
distinguish
orthotopic
hepatocellular
carcinoma
tissues
adjacent
mice
models
clinical
samples.
This
work
highlights
innovative
develop
rapid
diagnosis
tumors
high-precision
boundaries,
providing
more
efficient
tools
future
applications
assisted
resection.
Chemical Science,
Год журнала:
2024,
Номер
15(15), С. 5681 - 5693
Опубликована: Янв. 1, 2024
A
NIR
fluorescent
probe
for
monitoring
senescence
was
developed
with
SA-β-gal
and
HClO
as
targets
under
high
lysosomal
pH
conditions.
The
precise
tracking
of
cellular/
in
vivo
achieved
a
ratiometric
sensing
pattern.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(28), С. 10481 - 10485
Опубликована: Июль 3, 2023
β-Galactosidase
(β-gal)
is
the
gold
standard
marker
of
cellular
senescence,
which
linked
with
various
age-related
diseases.
Therefore,
it
essential
to
develop
more
excellent
probes
that
can
real-time
monitor
β-gal
activity
in
senescence
vivo.
Fluorescent/photoacoustic
(FL/PA)
dual-modal
imaging
possesses
sensitivity
and
spatial
resolution.
To
our
knowledge,
there
has
been
no
tumor-targeted
FL/PA
probe
image
by
monitoring
we
developed
a
(Gal-HCy-Biotin)
for
β-gal-activatable
tumor
senescence.
Gal-HCy
without
biotin
used
as
control
probe.
Gal-HCy-Biotin
superior
due
higher
kinetic
parameter
than
vitro.
Moreover,
could
help
enter
accumulate
cells
signal.
In
detail,
or
senescent
4.6-fold
3.5-fold
FL
enhancement
4.1-fold
3.3-fold
PA
enhancement.
2.9-fold
1.7-fold
3.8-fold
1.3-fold
We
envision
will
be
applied
clinic.
Molecular
imaging
is
a
non-invasive
method
that
widely
used
for
visualization
and
detection
of
biological
events
at
cellular
or
molecular
levels.
Stimuli-responsive
linkers
can
be
selectively
cleaved
by
specific
biomarkers
desired
sites
to
release
activate
agents
are
appealing
tools
improve
the
specificity,
sensitivity,
efficacy
imaging.
This
review
summarizes
recent
advances
stimuli-responsive
their
application
in
imaging,
highlighting
potential
these
design
activatable
probes.
It
hoped
this
could
inspire
more
research
interests
development
responsive
associated
applications.
Chemical Society Reviews,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Enzyme-responsive,
multi-lock
optical
probes
exhibit
superior
detection
specificity
towards
certain
biomolecular
events
and
pathological
conditions,
offering
high
disease
diagnostic
accuracy
treatment
efficacy.
Abstract
Cellular
senescence
is
a
steady
state
of
cell
cycle
arrest
necessary
to
maintain
homeostasis
in
organisms.
However,
senescent
cells
may
cause
neighboring
healthy
cells,
inducing
the
onset
several
diseases,
such
as
inflammation,
neurological
disorders,
and
atherosclerosis.
Therefore,
early
detection
cellular
extremely
important.
β‐Galactosidase
(β‐gal),
critical
marker
senescence,
can
be
monitored
facilitate
diagnosis
aging‐related
diseases.
Furthermore,
β‐gal
mainly
found
lysosomes,
which
have
pH
value
about
4.5–5.5.
Here,
we
developed
near‐infrared
fluorescent
probe
(QMOH‐Gal)
for
tracking
vitro
vivo
via
β‐gal.
In
addition,
displayed
high
sensitivity
specificity
with
good
fluorescence
signal
acidity
range.
Subsequently,
this
QMOH‐Gal
was
successfully
employed
differentiate
between
normal
by
monitoring
not
only
realized
zebrafish
but
also
palbociclib‐induced
breast
tumor
senescence.
Overall,
shows
great
promise
an
effective
tool
imaging
studying
biology
aging
European journal of medical research,
Год журнала:
2024,
Номер
29(1)
Опубликована: Янв. 9, 2024
The
senescence-associated
secretory
phenotype
(SASP)
is
a
generic
term
for
the
secretion
of
cytokines,
such
as
pro-inflammatory
factors
and
proteases.
It
crucial
feature
senescent
cells.
SASP
induce
tissue
remodeling
immune
cell
recruitment.
Previous
studies
have
focused
on
beneficial
role
during
embryonic
development,
wound
healing,
healing
in
general,
immunoregulation
properties,
cancer.
However,
some
recent
identified
several
negative
effects
fracture
healing.
Senolytics
drug
that
selectively
eliminates
can
inhibit
function
cells
SASP,
which
has
been
found
to
positive
variety
aging-related
diseases.
At
same
time,
data
suggest
removing
may
promote
Here,
we
reviewed
latest
research
progress
about
illustrated
inflammatory
response
influence
This
review
aims
understand
aiming
provide
an
important
clinical
prevention
treatment
strategy
fracture.
Clinical
trials
senolytics
agents
are
underway
expected
clarify
effectiveness
their
targeted
therapy
clinic
future.
Meanwhile,
adverse
this
method
still
need
further
study.
Chemosensors,
Год журнала:
2025,
Номер
13(2), С. 74 - 74
Опубликована: Фев. 17, 2025
Senescence
is
an
intricate
physiological
progression
that
can
be
instigated
by
a
multiplicity
of
factors.
Aberrant
cellular
senescence
capable
precipitating
substantial
array
diseases.
During
chemotherapy,
drugs
typically
tend
to
gradually
accumulate
in
the
liver,
thereby
inducing
liver
and
leading
successive
deterioration
its
function.
β-galactosidase
(β-gal),
serving
as
significant
index
exploration
senescence,
has
attracted
considerable
attention.
In
this
study,
fluorescence/photoacoustic
(FL/PA)
biomodal
probe
(Gal-QCS)
was
developed
based
on
hemicyanine
fluorophore
for
imaging
β-gal
process
drug-induced
senescence.
Gal-QCS
demonstrates
rapid
responsiveness,
high
sensitivity,
remarkable
selectivity
detecting
aqueous
solutions.
After
incubation
with
β-gal,
fluorescence
signal
at
810
nm
significantly
increases,
concurrently,
photoacoustic
775
also
exhibits
increment.
Upon
induction
cell
camptothecin,
expeditiously
selectively
image
senescent
cells.
Moreover,
after
administering
mice
FL/PA
signals
livers
were
enhanced
10.53-fold
1.43-fold,
respectively.
This
work
robustly
substantiates
potential
application
prospects
biomarker.
