Controlled activation of a DNA walker driven by mismatched catalytic hairpin assembly for microRNA imaging in cancer cells and clinical samples

Sensors and Actuators B Chemical, Год журнала: 2025, Номер 437, С. 137747 - 137747

Опубликована: Апрель 10, 2025

Язык: Английский

---

An enzymatically activated self-powered and self-recycled strategy for in situ tumor cell-specific molecular imaging

Chemical Engineering Journal, Год журнала: 2024, Номер 489, С. 151226 - 151226

Опубликована: Апрель 15, 2024

Язык: Английский

---

Exogenous and Endogenous Dual-Activated Nanoladder for Precise Imaging of Mitochondrial Ferroptosis-Related Inhibition miRNA with Tumor Cell Specificity

Analytical Chemistry, Год журнала: 2024, Номер 96(19), С. 7550 - 7557

Опубликована: Май 6, 2024

Developing precise tumor cell-specific mitochondrial ferroptosis-related inhibition miRNA imaging methods holds enormous potential for anticancer drug screening and cancer treatment. Nevertheless, traditional amplification still tolerated the limited specificity because of "off-tumor" signal leakage resulting from their "always-active" sensing mode. To overcome this limitation, we herein developed a dual (exogenous 808 nm NIR light endogenous APE1) activated nanoladder with cell improved resolution. Exogenous light-activation can regulate signals within mitochondria, enzyme-activation confine to cells. Based on activation design, off-tumor were greatly reduced tumor-to-background contrast was enhanced an tumor/normal discrimination ratio, realizing miRNA.

Язык: Английский

---

Engineering of a Self-Regulatory Bidirectional DNA Assembly Circuit for Amplified MicroRNA Imaging

Analytical Chemistry, Год журнала: 2023, Номер 95(51), С. 18731 - 18738

Опубликована: Дек. 14, 2023

The engineering of catalytic hybridization DNA circuits represents versatile ways to orchestrate a complex flux molecular information at the nanoscale, with potential applications in DNA-encoded biosensing, drug discovery, and therapeutics. However, diffusive escape intermediates unintentional binding interactions remain an unsolved challenge. Herein, we developed compact, yet efficient, self-regulatory assembly circuit (SAC) for achieving robust microRNA (miRNA) imaging live cells through DNA-templated guaranteed hybridization. By integrating toehold strand preblocked palindromic fragment stem domain, proposed miniature SAC system allows reactant-to-template-controlled proximal hybridization, thus facilitating bidirectional-sustained localization-intensified signal amplification without undesired crosstalk. With condensed components low reactant complexity, amplifier realized high-contrast intracellular miRNA imaging. We anticipate that this simple template-controlled design can enrich clinical diagnosis prognosis toolbox.

Язык: Английский

---

Cell Membrane-Anchored AND Logic Gate Aptasensor for Tumor Cell-Specific Imaging with Improved Accuracy

Analytical Chemistry, Год журнала: 2024, Номер 96(37), С. 14775 - 14782

Опубликована: Сен. 5, 2024

Accurate and rapid imaging of tumor cells is vital importance for early cancer diagnosis intervention. Aptamer-based fluorescence sensors have become a potent instrument bioimaging, while false positives on-target off-tumors linked to single-biomarker aptasensors compromise the specificity sensitivity imaging. In this paper, we describe sequential response aptasensor precise cell identification that based on DNA "AND" logic gate. Specifically, sensor consists three single-stranded DNA, including P-strand can sensitively respond an acid environment, L-strand containing ATP aptamer sequence, R-strand target anchoring. These strands hybridize with one another create Y-shaped structure (named Y-ALGN). The in utilized anchor membrane primarily. Responding extracellular acidic environment (input 1), I-motif sequence forms tetramer so released from exposes binding sites L-strand. Extracellular ATP, as input 2, continuously operates complete computation. Upon both protons molecules, activated restored particular membrane. Benefiting computation capacity gate, Y-ALGN distinguish between MCF-7 normal high accuracy. As simple dual-stimuli-responsive strategy, nanodevice would offer fresh approach accurately diagnosing cells.

Язык: Английский

---

Endogenous Enzyme-activated AND-gate DNA Nanomachines for Intracellular miRNA Detection and Cell-selective Imaging

Talanta, Год журнала: 2024, Номер 283, С. 127087 - 127087

Опубликована: Окт. 21, 2024

Язык: Английский

---

Light and endogenous enzyme triggered plasmonic antennas for accurate subcellular molecular imaging with enhanced spatial resolution

Chemical Science, Год журнала: 2023, Номер 15(2), С. 566 - 572

Опубликована: Дек. 2, 2023

Light and endogenous APE1-triggered plasmonic antennas were developed for accurate tumor-specific subcellular molecular imaging with enhanced spatial resolution.

Язык: Английский

---

Biomarker Multiplexing with Rational Design of Nucleic Acid Probe Complex

Analysis & Sensing, Год журнала: 2024, Номер 4(5)

Опубликована: Июль 10, 2024

Abstract The expression profiles of intracellular biomarkers hold significance for understanding cellular biological functions and tracking pathological activities. Due to its programmability biocompatibility, extensive efforts have been devoted design various kinds nucleic acid probes biomarker detection. However, pinpointing a single could end up in false positive signal, delaying diagnosis. In this review, we present an overview current advances detection signal amplification techniques. We highlight strategies multiplexing with combination isothermal approaches. High specificity sensitivity are the two criteria desired probe, as challenges encountered by probe that operates efficiently systems. With higher identification accuracy, may be able move one step closer precision medicine.

Язык: Английский

---

Colorimetric detection of furin based on enhanced catalytic activity of G-quadruplex/hemin DNAzyme

Analytica Chimica Acta, Год журнала: 2024, Номер 1323, С. 343070 - 343070

Опубликована: Авг. 8, 2024

Язык: Английский

---

Enzymatically Cyclic Activated Biosensor Based on a Tetrahedral DNA Framework for Precise Tumor in Situ Molecular Imaging

ACS Sensors, Год журнала: 2024, Номер 9(10), С. 5302 - 5311

Опубликована: Окт. 10, 2024

The development of stimulus-responsive and amplification-based strategies is crucial for achieving improved spatial specificity enhanced sensitivity in tumor molecular imaging, addressing challenges such as off-tumor signal leakage limited biomarker content. Therefore, a cyclically activated enzymatic biosensor based on the modification an AP site within tetrahedral framework DNA (AP-tFNA) was rationally developed cell-specific imaging using endogenous enzyme apurinic/apyrimidinic endonuclease 1 (APE1) target, exhibiting superior high sensitivity. APE1, which predominantly localizes nucleus normal cells but exhibits cytosolic expression cancer cells, can specifically recognize cleave AP-tFNA, resulting separation fluorophore quenching group, thereby inducing fluorescence signal. Additionally, upon completion excision one APE1 released, initiating subsequent cycle hydrolytic cleavage reactions. experimental results demonstrated that AP-tFNA enables precise differentiation both

Язык: Английский

---