Nanozymes
are
a
class
of
nanomaterials
that
exhibit
catalytic
functions
analogous
to
those
natural
enzymes.
They
demonstrate
considerable
promise
in
the
biomedical
field,
particularly
treatment
bone
infections,
due
their
distinctive
physicochemical
properties
and
adjustable
activities.
Bone
infections
(e.g.,
periprosthetic
osteomyelitis)
challenging
treat
clinically.
Traditional
treatments
often
encounter
issues
related
drug
resistance
suboptimal
anti-infection
outcomes.
The
advent
nanozymes
has
brought
with
it
new
avenue
hope
for
infections.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 27, 2025
Early
and
portable
detection
of
pathogenic
bacteria
is
crucial
for
ensuring
food
safety,
monitoring
product
quality,
tracing
the
sources
bacterial
infections.
Moving
beyond
traditional
plate-culture
counting
methods,
analysis
active
components
offers
a
rapid
means
quantifying
bacteria.
Here,
metal-organic
framework
(MOF)-derived
NiCo-layered
double
hydroxide
nanosheets
(LDHs),
synthesized
via
Kirkendall
effect,
were
employed
as
highly
effective
oxidase
mimics
to
generate
reactive
oxygen
species
(ROS).
These
ROS
quickly
etched
gold
nanobipyramids
(Au
NBPs),
producing
vivid
multicolormetric
response.
Experimental
results
theoretical
calculations
indicated
that
exceptional
oxidase-like
activity
NiCo-LDHs
stemmed
from
presence
bimetallic
sites
vacancies
modulating
local
electronic
structure
LDHs.
Additionally,
β-galactosidase
(β-Gal),
biomarker
Escherichia
coli,
reacted
with
p-aminophenyl-β-d-galactopyranoside
(PAPG)
form
p-aminophenol
(PAP),
reducing
agent
which
consumes
ROS,
thereby
inhibiting
etching
Au
NBPs.
Furthermore,
three-dimensional
(3D)-printed
point-of-care
testing
(POCT)
shell
was
designed
device
visually
detect
β-Gal
E.
coli
in
conjugation
smartphones.
This
study
not
only
provides
novel
approach
rational
design
nanozymes
but
also
establishes
portably
visual
biosensing
platform
detecting
