Micropore Resistance Counting Platform for Multiplexed and Ultrasensitive Detection of Mycotoxins and Biomarkers

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 3, 2025

Development of a multiplexed and sensitive biosensing platform is priority for public health security. We report micropore resistance counting based on polystyrene microsphere size-based encoding Clostridium butyricum Argonaute (CbAgo) decoding ultrasensitive detection. Initially, we constructed target DNA-modified coding system counting. Subsequently, the precise recognition cleavage capabilities guide DNA-activated CbAgo protein enable encoded system. Changes in concentration microspheres are presented as signal readout. The demonstrated excellent performance detection three mycotoxins (with sensitivity range over 4 orders magnitude reaching pg/mL level) two inflammatory markers at pg/mL. Combining enzyme by with counting, developed highly tool wide-ranging potential applications such clinical diagnosis, food safety inspection, environmental monitoring.

Язык: Английский

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Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104882 - 104882

Опубликована: Янв. 1, 2025

Язык: Английский

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Atom‐Modified gDNA Enhances Cleavage Activity of TtAgo Enabling Ultra‐Sensitive Nucleic Acid Testing

Advanced Science, Год журнала: 2024, Номер 11(28)

Опубликована: Май 10, 2024

Abstract The DNA‐guided (gDNA) Argonaute from Thermus thermophilus (TtAgo) has little potential for nucleic acid detection and gene editing due to its poor dsDNA cleavage activity at relatively low temperature. Herein, the of TtAgo is enhanced by using 2′‐fluorine (2′F)‐modified gDNA developes a novel testing strategy. This study finds that with 2′F‐nucleotides 3′‐end (2′F‐gDNA) can promote assembly TtAgo‐guide‐target ternary complex significantly increasing intermolecular force target DNA TtAgo, thereby providing ≈40‐fold enhancement decreasing minimum reaction temperature 65 60 °C. Based on this outstanding advance, strategy proposed, termed FAST, which performed 2′F‐gDNA/TtAgo recognition combining it Bst polymerase amplification. By integrating G‐quadruplex Thioflavin T, FAST assay achieves one‐pot real‐time fluorescence analysis ultra‐sensitivity, limit up 5 copies (20 µL mixture) miR‐21 detection. In summary, an atom‐modification‐based been developed enhancing efficiently, improving practicability establishing TtAgo‐based technology ultra‐sensitivity high‐specificity.

Язык: Английский

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Versatile sensing strategies based on emerging programmable prokaryotic Argonautes: From nucleic acid to non-nucleic acid targets

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118130 - 118130

Опубликована: Янв. 1, 2025

Язык: Английский

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EXPAR for Biosensing: Recent Developments and Applications

The Analyst, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Emerging as a promising novel amplification technique, the exponential reaction (EXPAR) offers significant advantages due to its potent capability, straightforward design, rapid kinetics, and isothermal operation. The past few years have witnessed swift advancements refinements in EXPAR-based technologies, with numerous high-performance biosensing systems documented. A deeper understanding of EXPAR mechanism has facilitated proposal strategies overcome limitations inherent traditional EXPAR. Furthermore, synergistic integration diverse methodologies, including use CRISPR/Cas system, metal nanoparticles, aptamers, alternative techniques, enzymes, significantly bolstered analytical efficacy, aiming enhance specificity, sensitivity, efficiency. This comprehensive review presents detailed exposition analyzes primary challenges. Additionally, we summarize latest research biomedical field concerning technologies for sensing strategies. Finally, discuss challenges future prospects technology realms clinical applications.

Язык: Английский

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Argonaute protein powered biosensing for pathogenic biosafety

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 305, С. 141321 - 141321

Опубликована: Фев. 19, 2025

Язык: Английский

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Programmable and ultra-efficient Argonaute protein-mediated nucleic acid tests: A review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 278, С. 134755 - 134755

Опубликована: Авг. 13, 2024

Язык: Английский

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Recent Advances in Exponential Amplification Reaction-Enabled Biosensors

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 181, С. 118006 - 118006

Опубликована: Окт. 13, 2024

Язык: Английский

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A DNA sensor based on CbAgo effector protein and on a dual electrochemical signal amplification strategy for B19 parvovirus detection

Bioelectrochemistry, Год журнала: 2024, Номер 162, С. 108860 - 108860

Опубликована: Ноя. 26, 2024

Язык: Английский

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Trends in the use of Argonaute Proteins in Molecular Diagnosis

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118081 - 118081

Опубликована: Ноя. 1, 2024

Язык: Английский

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