ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
10(11), С. 6814 - 6827
Опубликована: Окт. 5, 2024
DNA
is
a
kind
of
flexible
and
versatile
biomaterial
for
constructing
nanostructures
nanodevices.
Due
to
high
biocompatibility
programmability
easy
modification
fabrication,
nanotechnology
has
emerged
as
powerful
tool
application
in
intracellular
targeted
protein
degradation.
In
this
review,
we
summarize
the
recent
advances
design
mechanism
degradation
technologies
such
hydrolysis
chimeras,
lysosomal
autophagy
based
Subsequently,
introduce
nanotechnologies
cascade
circuits,
nanostructures,
dynamic
machines.
Moreover,
present
latest
developments
Finally,
vision
challenges
are
discussed.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Dynamic
DNA
nanodevices,
known
for
their
high
programmability
and
controllability,
are
pivotal
in
intracellular
biomarker
imaging.
However,
these
nanodevices
often
suffer
from
inadequate
detection
sensitivity
specificity
due
to
limited
cellular
loading
capacity
low
signal
feedback.
Herein,
we
engineered
an
integrated
multi-site
recognition
multi-signal
output
of
four-leaf
clover
dynamic
nanodevice
(MEMORY)
that
enables
sensitive
accurate
long
noncoding
RNA
(lncRNA)
MEMORY
features
one
fluorophore
(FAM)-modified
cross-shaped
structure
as
spatial-confinement
scaffolds
loaded
with
four
identical
quenchers
(BHQ1)-modified
probes
(RPs),
ensuring
a
background
initially.
In
the
presence
target
lncRNA,
multiple
sites
facilitate
hybridization
selectively
release
RPs,
exposing
toehold
region
outputting
green
fluorescence
(FAM)
signal.
Furthermore,
exposed
can
trigger
efficient
rapid
chain
reaction
(HCR)
amplification,
red
(Cy5)
MEMORY's
increase
likelihood
collisions,
enhancing
efficiency,
while
its
provides
sequential
feedback
through
FAM
Cy5,
boosting
overall
intensity.
With
lncRNA
metastasis-related
lung
adenocarcinoma
transcript
1
(MALAT1)
model,
offers
linear
range
pM
100
nM,
limit
0.29
pM.
We
demonstrated
differentiate
between
normal
tumor
cells
based
on
MALAT1
This
will
offer
valuable
tools
imaging
biomarkers.
Extracellular
vesicles
(EVs)
are
highly
attractive
in
cancer
diagnosis,
owing
to
their
ability
reflect
the
molecular
characteristics
of
source
cells.
Herein,
we
engineer
a
DNA-equipped
liposome
nanoprobe
for
developing
nanosized
membrane
fusion-triggered
three-dimensional
(3D)
DNA
walker
analysis
breast
EVs.
Specifically,
nanoprobes
efficiently
fuse
with
target
EVs
by
recognizing
surface-expressed
epidermal
growth
factor
receptors,
thereby
creating
3D
tracks
walker.
Subsequently,
walking
probes
targeting
human
receptor-2
(HER-2)
were
recruited
onto
fused
manipulate
walker,
eventually
generating
considerable
electrochemical
signals.
Results
reveal
good
linearity
between
output
and
EV
concentration
also
prove
differentiate
HER-2-positive
patients
monitor
disease
progression.
Taken
together,
this
work
presents
an
effective
approach
early
subtype-specific
diagnosis
cancer.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 20, 2025
Traditional
organic
planar
aromatic
emitters
often
encounter
low
electrochemiluminescence
(ECL)
efficiency
due
to
the
aggregation-induced
quenching
(ACQ)
induced
by
π-π
stacking,
limiting
their
applicability
in
bioanalysis.
In
this
work,
we
proposed
a
steric
engineering
strategy
mitigate
ACQ
perylene
diimide-bridged
β-cyclodextrin
aggregates
(Pe-CD
aggregates),
significantly
boosting
ECL
for
trace
microRNA
(miRNA)
detection.
By
incorporation
of
large
volume
cyclic
(β-CD)
at
both
side
chains
diimide,
Pe-CD
molecules
were
generated
and
further
assembled
into
robust
supramolecular
nanoblocks.
This
modulates
intermolecular
distances
around
cores,
effectively
minimizing
nonradiative
relaxation.
Molecular
simulation
results
demonstrated
that
distance
increased
from
3.32
Å
5.21
Å,
accompanied
5.8-fold
enhancement
compared
Pe
(formed
dense
stacking
molecules).
As
proof
concept,
an
bioassay
platform
was
developed
using
as
target-enrichment
accelerated-3D
DNAzyme
walkers
signal
amplifiers
detecting
miRNA-21,
achieving
limit
detection
9.7
aM.
study
offers
valuable
insights
designing
new
expands
potential
applications
technology.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(41), С. 16415 - 16424
Опубликована: Окт. 3, 2024
The
detection
of
DNA
methyltransferase
(MTase)
was
crucial
for
understanding
gene
expression
regulation,
cancer
mechanisms,
and
various
biological
processes,
contributing
significantly
to
disease
diagnosis
drug
development.
Herein,
a
nanopore
sensor
based
on
cascaded
signal
amplification
walker
autocatalytic
hybridization
reaction
(AHR)
developed
the
ultrasensitive
determination
MTases.
In
presence
Dam
MTase,
hairpin
structure
H
