Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

In this work, using Al-H3NTB-MOG with self-enhanced and aggregation-induced electrochemiluminescence (AIECL) performance as an efficient emitter, a biosensor based on Y-shaped multiregion dual-drive DNA walker was constructed for the sensitive detection of miRNA-141. Notably, 4,4',4″-nitrilotribenzoic acid (H3NTB) selected luminescent ligand ECL property co-reactive tertiary amine in its structure. Al3+ served central ion to coordinate H3NTB form three-dimensional porous gel structure, which restricted internal rotation vibration benzene molecules exhibited excellent AIECL property. More interestingly, N-2-hydroxyethylpiperazine-N'-ethane-sulfonic (HEPES) chosen system buffer, could not only stabilize test environment but also play co-reaction compensation role compensate consumption groups process, then significantly resulting better stability response. Besides, dynamic signal amplification established synergistic effect rolling cycle (RCA) process ionic cleavage at both ends nanostructure assembled by catalytic hairpin self-assembly (CHA) reaction. Specifically, two long chains abundant recognition regions were formed RCA reaction walker, simultaneously walk along predesigned tracks shear specific sites from directions, effectively improving efficiency. that way, realized miRNA-141 10 aM 1 nM range limit low 6.48 aM.

Язык: Английский