A dual-switch fluorescence biosensor with entropy-driven and DNA walker cascade amplification circuit for sensitive microRNA detection DOI
Zhouqing Xie, Zhao‐You Tang, Xuliang Zhuang

и другие.

Nanoscale, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

To achieve precise diagnosis of tumor cells, designing nucleic acid amplification circuits with intelligent multi-switch responsiveness for the specific and sensitive detection miRNA within cells is an important strategy. Here, we developed a dual-switch fluorescence biosensor that integrates two-step cascade signal circuit entropy-driven (EDC) DNA walkers onto gold nanoparticles highly quantitative target in cells. The dual switches trigger are APE1 enzyme, both which upregulated be specific, miRNA21 triggers upstream EDC, releasing strands serve as downstream circuit. Under cleavage-driven action walker walk along track on surface AuNPs, strong presenting good linearity concentration range 40 pM-100 nM. This presents specificity, anti-interference ability against multiple RNAs enzymes can effectively distinguish cancer from normal cell lysates. Overall, this provides recognition efficient strategy tumors, indicating its potential clinical applications disease diagnosis.

Язык: Английский

A dual-switch fluorescence biosensor with entropy-driven and DNA walker cascade amplification circuit for sensitive microRNA detection DOI
Zhouqing Xie, Zhao‐You Tang, Xuliang Zhuang

и другие.

Nanoscale, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

To achieve precise diagnosis of tumor cells, designing nucleic acid amplification circuits with intelligent multi-switch responsiveness for the specific and sensitive detection miRNA within cells is an important strategy. Here, we developed a dual-switch fluorescence biosensor that integrates two-step cascade signal circuit entropy-driven (EDC) DNA walkers onto gold nanoparticles highly quantitative target in cells. The dual switches trigger are APE1 enzyme, both which upregulated be specific, miRNA21 triggers upstream EDC, releasing strands serve as downstream circuit. Under cleavage-driven action walker walk along track on surface AuNPs, strong presenting good linearity concentration range 40 pM-100 nM. This presents specificity, anti-interference ability against multiple RNAs enzymes can effectively distinguish cancer from normal cell lysates. Overall, this provides recognition efficient strategy tumors, indicating its potential clinical applications disease diagnosis.

Язык: Английский

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