Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 21, 2025

The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) system has achieved remarkable success in the field of nucleic acid detection, while its Achilles' heel lies difficulties encountered flexibility regarding multiplex detection. As a sister CRISPR-Cas, prokaryotic Argonautes (pAgos) have precise recognition, multiturnover, and more importantly multiple specific cleavage characteristics, which is potential candidate for next generation Herein, single particle counting platform was developed simultaneous detection three colorectal cancer-related miRNAs (miR-141, miR-31, miR-21) by combining inductively coupled plasma mass spectrometry (SP-ICPMS) with Thermus thermophilus Argonaute protein (TtAgo), nanoparticles as signal probes cleavage. demonstrated high sensitivity (aM level) specificity due to dual-cycle mechanism exponential isothermal amplification (EXPAR) TtAgo cleavage, well combination TtAgo's capability advantages metal stable isotope tagging. Additionally, showed good robustness human serum cell extracts, indicating significant clinical applications.

Язык: Английский