Multi-defense Pathways against Electrophiles through Adduct Formation by Low Molecular Weight Substances with Sulfur Atoms DOI Creative Commons
Yoshito Kumagai,

Yumi Abiko,

Masahiro Akiyama

и другие.

Toxicological Sciences, Год журнала: 2024, Номер unknown

Опубликована: Окт. 7, 2024

There is a variety of electrophiles in the environment. In addition, there are precursor chemicals that undergo metabolic activation by enzymes and conversion to body. Although covalently bind protein nucleophiles, they also form adducts associated with adaptive or toxic responses. Low molecular weight compounds containing sulfur capable blocking such adduct formation capturing electrophiles. this review, we present out findings on capture inactivation 1) intracellular glutathione, 2) reactive species 3) extracellular cysteine (formed during production adducts). These actions not only substantially suppress electrophilic activity but regulate formation.

Язык: Английский

Analysis and characterization of sulfane sulfur DOI

Shin Koike,

Yuki Ogasawara

Analytical Biochemistry, Год журнала: 2024, Номер 687, С. 115458 - 115458

Опубликована: Янв. 4, 2024

Язык: Английский

Процитировано

6
Extracellularly secreted cysteine derived from cystine regulates oxidative and electrophilic stress in HepG2 cells DOI Creative Commons

Hanako Aoki,

Yasuhiro Shinkai, Masahiro Akiyama

и другие.

Free Radical Research, Год журнала: 2024, Номер 58(5), С. 323 - 332

Опубликована: Май 3, 2024

While cysteine (CysSH) is known to be exported into the extracellular space, its biological significance not well understood. The present study examined movement of CysSH using stable isotope-labeled cystine (CysSSCys), which transported cells and reduced CysSH. Exposure HepG2 100 µM CysSSCys resulted in 70 labeled cell medium 1 h after exposure. When was collected incubated with either hydrogen peroxide (H2O2) or atmospheric electrophiles, such as 1,2-naphthoquinone, 1,4-naphthoquinone 1,4-benzoquinone, almost completely consumed. In contrast, levels were unaltered during exposure H2O2 for up 2 h, suggesting redox cycling CysSSCys/CysSH system. Experiments without changing containing from revealed that oxidative electrophilic modifications cellular proteins, caused by significantly repressed medium. We also participation enzymes and/or antioxidants intracellular reduction These results provide new findings derived plays a role regulation stress.

Язык: Английский

Процитировано

1
Multi-defense Pathways against Electrophiles through Adduct Formation by Low Molecular Weight Substances with Sulfur Atoms DOI Creative Commons
Yoshito Kumagai,

Yumi Abiko,

Masahiro Akiyama

и другие.

Toxicological Sciences, Год журнала: 2024, Номер unknown

Опубликована: Окт. 7, 2024

There is a variety of electrophiles in the environment. In addition, there are precursor chemicals that undergo metabolic activation by enzymes and conversion to body. Although covalently bind protein nucleophiles, they also form adducts associated with adaptive or toxic responses. Low molecular weight compounds containing sulfur capable blocking such adduct formation capturing electrophiles. this review, we present out findings on capture inactivation 1) intracellular glutathione, 2) reactive species 3) extracellular cysteine (formed during production adducts). These actions not only substantially suppress electrophilic activity but regulate formation.

Язык: Английский

Процитировано

0