JACS Au,
Год журнала:
2022,
Номер
2(11), С. 2381 - 2399
Опубликована: Окт. 21, 2022
Artificial
molecular
machines
have
found
widespread
applications
ranging
from
fundamental
studies
to
biomedicine.
More
recent
advances
in
exploiting
unique
physical
and
chemical
properties
of
DNA
led
the
development
DNA-based
artificial
machines.
The
unprecedented
programmability
provides
a
powerful
means
design
complex
sophisticated
that
can
exert
mechanical
force
or
motion
realize
tasks
controllable,
modular
fashion.
This
Perspective
highlights
potential
strategies
construct
using
double-stranded
DNA,
functional
nucleic
acids,
frameworks,
which
enable
improved
control
over
reaction
pathways
behaviors.
We
also
outline
challenges
opportunities
for
biophysics,
biosensing,
biocomputing.
ACS Nano,
Год журнала:
2024,
Номер
18(46), С. 31713 - 31736
Опубликована: Ноя. 7, 2024
Sensitive
and
quantitative
detection
of
chemical
biological
molecules
for
screening,
diagnosis
monitoring
diseases
is
essential
to
treatment
planning
response
monitoring.
Electrochemical
biosensors
are
fast,
sensitive,
easy
miniaturize,
which
has
led
rapid
development
in
clinical
diagnosis.
Benefiting
from
their
excellent
molecular
recognition
ability
high
programmability,
DNA
nanomaterials
could
overcome
the
Debye
length
electrochemical
by
simple
design
well
suited
as
elements
biosensors.
Therefore,
enhance
sensitivity
specificity
biosensors,
significant
progress
been
made
recent
years
optimizing
design.
Here,
establishment
sensing
strategies
based
on
reviewed
detail.
First,
structural
nanomaterial
examined
improving
overcoming
length.
In
addition,
electrical
signal
transduction
amplification
reviewed,
applications
nanomaterial-based
integrated
devices
further
summarized.
Finally,
main
opportunities
challenges
detecting
disease
biomarkers
presented
an
aim
guide
with
specificity.
Rapid
and
accurate
molecular
diagnostics
are
crucial
for
preventing
the
global
spread
of
emerging
infectious
diseases.
However,
current
gold
standard
nucleic
acid
detection,
reverse
transcription
polymerase
chain
reaction
(RT-PCR),
relies
heavily
on
traditional
magnetic
beads
or
silica
membranes
extraction,
resulting
in
several
limitations,
including
time-consuming
processes,
need
trained
personnel,
complex
equipment.
Therefore,
there
is
an
urgent
fully
integrated
detection
technologies
that
simple
to
operate,
rapid,
highly
sensitive
meet
unmet
clinical
needs.
In
this
study,
we
developed
a
novel,
microfluidic
cartridge
featuring
unique
needle-plug/piston
microvalve,
which
enables
stable
long-term
reagent
storage
flexible
liquid
handling
on-site
analysis.
Coupled
with
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Фев. 21, 2022
Shigella
flexneri
is
a
serious
threat
to
global
public
health,
and
rapid
detection
method
urgently
needed.
The
CRISPR/Cas
(clustered
regularly
interspaced
short
palindromic
repeats/CRISPR-associated)
system
widely
used
in
gene
editing,
therapy,
vitro
diagnosis.
Here,
we
combined
loop-mediated
isothermal
amplification
(LAMP)
CRISPR/Cas12a
develop
novel
diagnostic
test
(CRISPR/Cas12a-E-LAMP)
for
the
diagnosis
of
S.
.
CRISPR/Cas12a-E-LAMP
protocol
conducts
LAMP
reaction
templates
followed
by
predefined
target
sequences.
primers
sgRNAs
were
designed
highly
conserved
hypothetical
protein
(accession:
AE014073,
region:
4170556–4171,068)
After
at
60°C
20
min,
pre-loaded
regents
mixed
with
products
one
tube
37°C
final
results
can
be
viewed
naked
eyes
total
time
40
min.
sensitivity
detect
was
4
×
10
0
copies/μl
plasmids
without
cross-reaction
other
six
closely
related
non-
flexneri.
Therefore,
assay
useful
reliable
quick
may
applied
pathogen
infection
detection.
JACS Au,
Год журнала:
2022,
Номер
2(11), С. 2381 - 2399
Опубликована: Окт. 21, 2022
Artificial
molecular
machines
have
found
widespread
applications
ranging
from
fundamental
studies
to
biomedicine.
More
recent
advances
in
exploiting
unique
physical
and
chemical
properties
of
DNA
led
the
development
DNA-based
artificial
machines.
The
unprecedented
programmability
provides
a
powerful
means
design
complex
sophisticated
that
can
exert
mechanical
force
or
motion
realize
tasks
controllable,
modular
fashion.
This
Perspective
highlights
potential
strategies
construct
using
double-stranded
DNA,
functional
nucleic
acids,
frameworks,
which
enable
improved
control
over
reaction
pathways
behaviors.
We
also
outline
challenges
opportunities
for
biophysics,
biosensing,
biocomputing.
