Biotechnology Journal,
Год журнала:
2025,
Номер
20(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Terpenoids
are
widely
distributed
in
nature
and
have
various
applications
healthcare
products,
pharmaceuticals,
fragrances.
Despite
the
significant
potential
that
terpenoids
possess,
traditional
production
methods,
such
as
plant
extraction
chemical
synthesis,
face
challenges
meeting
current
market
demand.
With
advancement
of
synthetic
biology
metabolic
engineering,
it
becomes
feasible
to
construct
efficient
microbial
cell
factories
for
large‐scale
terpenoids.
This
article
primarily
centers
on
heterologous
expression
Saccharomyces
cerevisiae
,
detailing
terpenoid
biosynthesis
pathways
through
utilization
cellular
microcompartments,
strategies
key
P450
enzymes
synthesis
pathway,
regulation
optimization
host
metabolism
enhance
flux
synthesis.
Additionally,
we
analyze
propose
solutions
further
refine
yeast
chassis
more
effective
production.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Янв. 2, 2024
Abstract
Artificial
organelles
can
manipulate
cellular
functions
and
introduce
non-biological
processes
into
cells.
Coacervate
droplets
have
emerged
as
a
close
analog
of
membraneless
organelles.
Their
biomimetic
properties,
such
molecular
crowding
selective
partitioning,
make
them
promising
components
for
designing
cell-like
materials.
However,
their
use
artificial
has
been
limited
by
complex
structure,
control
over
internal
microenvironment
inherent
colloidal
instability.
Here
we
report
the
design
dipeptide
coacervates
that
exhibit
enhanced
stability,
biocompatibility,
hydrophobic
microenvironment.
The
character
facilitates
encapsulation
species,
including
transition
metal-based
catalysts,
enhancing
efficiency
in
aqueous
environments.
Dipeptide
carrying
catalyst
are
incorporated
active
cells
trigger
an
chemical
reaction.
development
with
opens
alternative
avenue
field
materials
applications
catalysis
synthetic
biology.
Chemical Reviews,
Год журнала:
2024,
Номер
124(5), С. 2553 - 2582
Опубликована: Март 4, 2024
The
intricate
and
complex
features
of
enzymatic
reaction
networks
(ERNs)
play
a
key
role
in
the
emergence
sustenance
life.
Constructing
such
vitro
enables
stepwise
build
up
complexity
introduces
opportunity
to
control
activity
using
physicochemical
stimuli.
Rational
design
modulation
network
motifs
enable
engineering
artificial
systems
with
emergent
functionalities.
Such
functional
are
useful
for
variety
reasons
as
creating
new-to-nature
dynamic
materials,
producing
value-added
chemicals,
constructing
metabolic
modules
synthetic
cells,
even
enabling
molecular
computation.
In
this
review,
we
offer
insights
into
chemical
characteristics
ERNs
while
also
delving
their
potential
applications
associated
challenges.
Chemical Society Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
We
explore
how
simulations
and
modelling
help
us
to
learn
about
the
fundamental
principles
of
DNA
self-assembly;
we
highlight
key
concepts
such
as
cooperativity
nucleation
show
they
can
be
harnessed
improve
self-assembly
process.
PLANT PHYSIOLOGY,
Год журнала:
2024,
Номер
195(1), С. 36 - 47
Опубликована: Янв. 2, 2024
Abstract
The
whole
field
of
synthetic
biology
(SynBio)
is
only
about
20
years
old,
and
plant
SynBio
younger
still.
Nevertheless,
within
that
short
time,
in
general
has
drawn
more
scientific,
philosophical,
government,
private-sector
interest
than
anything
since
the
recombinant
DNA
revolution.
Plant
SynBio,
particular,
now
drawing
relation
to
plants’
potential
help
solve
planetary
problems
such
as
carbon
capture
storage
replacing
fossil
fuels
feedstocks.
As
so
young
fast-developing,
we
felt
it
was
too
soon
try
analyze
its
history.
Instead,
set
out
essence
SynBio's
origins
early
development
through
interviews
with
8
field's
founders,
representing
5
countries
3
continents.
We
then
distilled
these
founders’
personal
recollections
reflections
into
this
review,
centering
narrative
on
timelines
for
pivotal
events,
articles,
funding
programs,
quoting
from
interviews.
have
archived
interview
recordings
documented
timeline
entries.
This
work
provides
a
resource
future
historical
scholarship.
Molecules,
Год журнала:
2024,
Номер
29(9), С. 2106 - 2106
Опубликована: Май 2, 2024
Diverse
secondary
metabolites
in
plants,
with
their
rich
biological
activities,
have
long
been
important
sources
for
human
medicine,
food
additives,
pesticides,
etc.
However,
the
large-scale
cultivation
of
host
plants
consumes
land
resources
and
is
susceptible
to
pest
disease
problems.
Additionally,
multi-step
demanding
nature
chemical
synthesis
adds
production
costs,
limiting
widespread
application.
In
vitro
metabolic
engineering
significantly
enhanced
successful
industrial
cases.
As
synthetic
biology
advances,
more
research
focusing
on
heterologous
using
microorganisms.
This
review
provides
a
comprehensive
comparison
between
these
two
chassis,
evaluating
performance
various
types
from
perspectives
yield
strategies.
It
also
discusses
challenges
they
face
offers
insights
into
future
efforts
directions.
Communications Chemistry,
Год журнала:
2023,
Номер
6(1)
Опубликована: Март 28, 2023
A
"synthetic
minimal
cell"
is
considered
here
as
a
cell-like
artificial
vesicle
reproduction
system
in
which
chemical
and
physico-chemical
transformation
network
regulated
by
information
polymers.
Here
we
synthesise
such
cell
consisting
of
three
units:
energy
production,
polymer
synthesis,
reproduction.
Supplied
ingredients
are
converted
to
currencies
trigger
the
synthesis
an
polymer,
where
membrane
plays
role
template.
The
promotes
growth.
By
tuning
composition
permeability
osmolytes,
growing
vesicles
show
recursive
over
several
generations.
Our
greatly
simplifies
scheme
contemporary
living
cells
while
keeping
their
essence.
pathways
well
described
kinetic
equations
applying
elasticity
model,
respectively.
This
study
provides
new
insights
better
understand
differences
similarities
between
non-living
forms
matter
life.
Chemical Science,
Год журнала:
2024,
Номер
15(28), С. 10724 - 10744
Опубликована: Янв. 1, 2024
This
tutorial
review
gives
an
overview
over
the
different
lipid
–
block
copolymer
hybrid
vesicle
designs,
and
provides
a
detailed
analysis
of
their
properties.
It
puts
emphasis
on
application
in
bottom-up
synthetic
biology.
