Abstract
Tissue‐engineered
bone
has
emerged
as
a
promising
alternative
for
defect
repair
due
to
the
advantages
of
regenerative
healing
and
physiological
functional
reconstruction.
However,
there
is
very
limited
breakthrough
in
achieving
favorable
regeneration
harsh
osteogenic
microenvironment
after
injury,
especially
avascular
hypoxic
conditions.
Inspired
by
developmental
mode
endochondral
ossification,
novel
strategy
proposed
tolerant
rapid
using
framework‐enhanced
3D
biomineralized
matrix
hydrogels.
First,
it
meticulously
designed
biomimetic
hydrogels
with
both
osteoinductive
microenvironment,
then
integrated
3D‐printed
polycaprolactone
framework
improve
their
mechanical
strength
structural
fidelity.
The
inherent
effectively
activates
marrow
mesenchymal
stem
cells
self‐regulation
early‐stage
chondrogenesis
via
TGFβ/Smad
signaling
pathway
obstacle
aerobic
respiration.
Meanwhile,
strong
created
hybrid
formulation
native‐constitute
inorganic
salts,
can
synergistically
regulate
mineralization
osteoclastic
differentiation,
thus
accelerate
late‐stage
maturation.
Furthermore,
vivo
ectopic
osteogenesis
situ
skull
successfully
verified
high
efficiency
maintenance
mode,
which
offers
treatment
craniofacial
repair.
ACS Applied Bio Materials,
Год журнала:
2024,
Номер
7(5), С. 2982 - 2992
Опубликована: Апрель 8, 2024
Mycelium
is
the
root-like
network
of
fungi.
biocomposites
prepared
by
template
replication
(molding)
can
function
as
environmentally
friendly
alternatives
to
conventional
polystyrene
foams,
which
are
energy-
and
carbon-intensive
manufacture.
Recently,
several
studies
have
shown
that
3D
bioprinting
technologies
be
used
produce
high
value
functional
mycelium
products
with
intricate
geometries
otherwise
difficult
or
impossible
achieve
via
replication.
A
diverse
range
nutrients,
thickeners,
gelling
agents
combined
hydrogels
suitable
for
bioprinting.
hydrogel
formulations
infused
living
fungi
produces
engineered
materials
continue
grow
after
complete.
However,
a
formulation
optimized
Pleurotus
ostreatus
mycelium,
among
strains
most
commonly
in
biocomposite
fabrication,
has
yet
described.
Here,
we
design
evaluate
versatile
consisting
malt
extract
(nutrient),
carboxymethylcellulose
cornstarch
(thickeners),
agar
(gelling
agent),
all
easily
sourced
food
grade
reagents.
We
also
outline
reproducible
workflow
infuse
this
P.
liquid
culture
structures
comprised
characterize
changes
height
mass
well
hardness
prints
during
growth.
Finally,
demonstrate
does
not
require
sterile
environment
successful
same
mycelium-infused
supplemented
additives
such
sawdust
objects.
These
findings
using
mycelium-based
feedstocks
could
promising
biofabrication
technique
applications
mushroom
cultivation,
preparation,
construction
built
environment.
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(14), С. 4603 - 4631
Опубликована: Янв. 1, 2023
This
review
highlights
the
design
principles
for
functional
amyloid
fibrillar
assemblies
from
an
engineering
perspective
as
well
through
lens
of
structural
insights.
Abstract
Tissue‐engineered
bone
has
emerged
as
a
promising
alternative
for
defect
repair
due
to
the
advantages
of
regenerative
healing
and
physiological
functional
reconstruction.
However,
there
is
very
limited
breakthrough
in
achieving
favorable
regeneration
harsh
osteogenic
microenvironment
after
injury,
especially
avascular
hypoxic
conditions.
Inspired
by
developmental
mode
endochondral
ossification,
novel
strategy
proposed
tolerant
rapid
using
framework‐enhanced
3D
biomineralized
matrix
hydrogels.
First,
it
meticulously
designed
biomimetic
hydrogels
with
both
osteoinductive
microenvironment,
then
integrated
3D‐printed
polycaprolactone
framework
improve
their
mechanical
strength
structural
fidelity.
The
inherent
effectively
activates
marrow
mesenchymal
stem
cells
self‐regulation
early‐stage
chondrogenesis
via
TGFβ/Smad
signaling
pathway
obstacle
aerobic
respiration.
Meanwhile,
strong
created
hybrid
formulation
native‐constitute
inorganic
salts,
can
synergistically
regulate
mineralization
osteoclastic
differentiation,
thus
accelerate
late‐stage
maturation.
Furthermore,
vivo
ectopic
osteogenesis
situ
skull
successfully
verified
high
efficiency
maintenance
mode,
which
offers
treatment
craniofacial
repair.
