Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 110960 - 110960
Опубликована: Фев. 1, 2025
Язык: Английский
Chinese Journal of Applied Physiology, Год журнала: 2025, Номер unknown, С. e20250001 - e20250001
Опубликована: Янв. 1, 2025
The aim of this study is to explore and evaluate recent innovations in drug delivery systems (DDS) for biologics, focusing on enhancing stability targeted improve the efficacy safety next-generation therapeutics. most developments a variety DDS, such as nanoparticles, microneedles, hydrogels, biodegradable polymers, were examined depth. Information from peer-audited diaries, clinical preliminaries, mechanical reports blended survey presentation these frameworks concerning dependability, designated conveyance, patient consistence, controlled discharge. A radar chart was used comparative analysis show advantages disadvantages each DDS. Utilizing cutting-edge our revealed significant improvements biologics. Nanoparticles exhibited elevated precision conveyance at 92% showed 85% improvement soundness. With an 88% satisfaction rate moderate other criteria, microneedles achieved highest level compliance. Biodegradable polymers provided balanced enhancement across all with stability, 87% delivery, 89% release hydrogels. lost only 6% their 10% release, hydrogels 7% 5% compliance systems. precise biologics have been significantly improved by advancements Hydrogels hand, provide nanoparticles are promising maintaining integrity targeting particular sites. In order overcome limitations that exist currently enhance therapeutic outcomes future research ought concentrate hybrid strategies combine multiple
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Янв. 16, 2025
The dysregulation of matrix metalloproteinases (MMPs) in skin cutaneous melanoma (SKCM) represents a critical aspect tumorigenesis. In this study, we investigated the diagnostic, prognostic, and therapeutic aspects MMPs SKCM. Thirteen SKCM cell lines seven normal were cultured under standard conditions for experimental analyses. RNA DNA extracted, followed by RT-qPCR to assess MMP expression promoter methylation analysis determine levels. Functional assays, including proliferation, colony formation, wound healing, conducted post-MMP7 knockdown using siRNA A375 cells. Databases like GEPIA2, HPA, MEXPRESS, miRNet employed expression, survival, methylation, miRNA-mRNA network We landscape lines, revealing significant (p-value < 0.05) up-regulation MMP1, MMP7, MMP9, MMP10, MMP11, MMP12, MMP13, MMP14, MMP25, alongside down-regulation MMP2, MMP3, MMP21. Furthermore, our demonstrated inverse correlation between levels status, suggesting potential regulatory role dysregulation. Notably, MMP14 exhibited associations with overall survival patients, emphasizing their prognostic significance. Additionally, Receiver operating characteristic (ROC) curve highlighted diagnostic distinguishing from individuals. Subsequent validation across multiple cohorts confirmed elevated tissues, particularly advanced disease stages, further tumor progression. elucidated pathways involving miR-22-3p, which targets genes Our findings also revealed immune modulation, drug sensitivity, functional states Lastly, MMP7 cells significantly impacted several characteristics, healing. highlight These could serve as biomarkers early detection therapy. Future efforts should focus on preclinical clinical translate these insights into personalized strategies.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Фев. 14, 2025
Nanobodies have gained significant attention as promising tools for cancer diagnostics and treatment due to their unique ability precisely target specific cells. However, a major challenge lies in the site-specific incorporation of multifunctional molecules into nanobodies, it is essential link these manner that preserves nanobody's function stability while retaining desired therapeutic or diagnostic properties. This study outlines development dual-functional nanobody optical probes enhanced interventions. We designed clickable linker enables functionalization nanobody, facilitating simultaneous conjugation two dyes: indocyanine green imaging chlorin e6 photodynamic therapy. In vitro cellular assays confirmed successful labeling dyes, with probe exhibiting high binding specificity. vivo mice bearing Hep3B tumors revealed clear visualization signal-to-noise ratio. Furthermore, PEGylated significantly improved tumor retention, enhancing both contrast therapy efficacy compared free e6. These show great promise precise diagnosis malignant tumors.
Язык: Английский
Процитировано
0
Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 110960 - 110960
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0