Testing at scale during the COVID-19 pandemic

Nature Reviews Genetics, Год журнала: 2021, Номер 22(7), С. 415 - 426

Опубликована: Май 4, 2021

Язык: Английский

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Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

The Science of The Total Environment, Год журнала: 2021, Номер 805, С. 149877 - 149877

Опубликована: Авг. 25, 2021

Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in wastewater can potentially provide early warning signal COVID-19 infections a community. The capacity world's environmental microbiology virology laboratories SARS-CoV-2 RNA characterization increasing rapidly. However, there are no standardized protocols or harmonized quality assurance control (QA/QC) procedures surveillance. This paper technical review factors that cause false-positive false-negative errors wastewater, culminating recommended strategies be implemented to identify mitigate some these errors. Recommendations include stringent QA/QC measures, representative sampling approaches, virus concentration efficient extraction, PCR inhibition assessment, inclusion sample processing controls, considerations RT-PCR assay selection data interpretation. Clear interpretation guidelines (e.g., determination positive negative samples) critical, particularly when low. Corrective confirmatory actions must place inconclusive results diverging from current trends initial onset reemergence community). It also prudent perform interlaboratory comparisons ensure results' reliability interpretability prospective retrospective analyses. this aim improve detection applications. A silver lining pandemic efficacy continues demonstrated during global crisis. In future, should play important role range other communicable diseases.

Язык: Английский

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Evaluation of Sampling, Analysis, and Normalization Methods for SARS-CoV-2 Concentrations in Wastewater to Assess COVID-19 Burdens in Wisconsin Communities

ACS ES&T Water, Год журнала: 2021, Номер 1(8), С. 1955 - 1965

Опубликована: Июль 9, 2021

Wastewater surveillance for SARS-CoV-2 provides an approach assessing the infection burden across a sewer service area. For these data to be useful public health, measurement variability and relationship case need established. We determined RNA concentrations in influent of 12 wastewater treatment plants from August 2020 January 2021. Technical replicates N1 gene showed relative standard deviation 24%, suggesting it is possible track relatively small (∼30%) changes over time. COVID-19 cases were correlated significantly (ρ ≥ 0.70) large areas, with weaker relationships 0.59) two communities. normalized per capita slightly improved correlations incidence, but normalizing spiked recovery control (BCoV) or fecal marker (PMMoV HF183) reduced number plants. Daily sampling demonstrated that minimum samples collected week needed maintain accuracy trend analysis. The differences strength incidence effect normalization on among communities demonstrate rigorous validation should performed at individual sites where programs are implemented.

Язык: Английский

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Catching a resurgence: Increase in SARS-CoV-2 viral RNA identified in wastewater 48 h before COVID-19 clinical tests and 96 h before hospitalizations

The Science of The Total Environment, Год журнала: 2021, Номер 770, С. 145319 - 145319

Опубликована: Янв. 22, 2021

Язык: Английский

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Wastewater surveillance to infer COVID-19 transmission: A systematic review

The Science of The Total Environment, Год журнала: 2021, Номер 804, С. 150060 - 150060

Опубликована: Сен. 8, 2021

Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.

Язык: Английский

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COVID-19 wastewater epidemiology: a model to estimate infected populations

The Lancet Planetary Health, Год журнала: 2021, Номер 5(12), С. e874 - e881

Опубликована: Дек. 1, 2021

Язык: Английский

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Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes

Journal of Hazardous Materials, Год журнала: 2021, Номер 424, С. 127456 - 127456

Опубликована: Окт. 9, 2021

The COVID-19 pandemic has put unprecedented pressure on public health resources around the world. From adversity, opportunities have arisen to measure state and dynamics of human disease at a scale not seen before. In United Kingdom, evidence that wastewater could be used monitor SARS-CoV-2 virus prompted development National surveillance programmes. pace this work proven unique in monitoring national level, demonstrating importance wastewater-based epidemiology (WBE) for protection. Beyond COVID-19, it can provide additional value informing range biological chemical markers health. A discussion measurement uncertainty associated with wastewater, focusing lessons-learned from UK programmes is presented, showing sources impacting quality interpretation data decision-making, are varied complex. While some factors remain poorly understood, we present approaches taken by manage mitigate more tractable uncertainty. This provides platform integrate management into WBE activities as part global One Health initiatives beyond pandemic.

Язык: Английский

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Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiology

Chemical Engineering Journal, Год журнала: 2021, Номер 415, С. 129039 - 129039

Опубликована: Фев. 21, 2021

Язык: Английский

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Longitudinal Monitoring of SARS-CoV-2 RNA on High-Touch Surfaces in a Community Setting

Environmental Science & Technology Letters, Год журнала: 2020, Номер 8(2), С. 168 - 175

Опубликована: Дек. 14, 2020

Environmental surveillance of surface contamination is an unexplored tool for understanding transmission SARS-CoV-2 in community settings. We conducted longitudinal swab sampling high-touch non-porous surfaces a Massachusetts town during COVID-19 outbreak from April to June 2020. Twenty-nine 348 (8.3%) samples were positive RNA, including crosswalk buttons, trash can handles, and door handles essential business entrances (grocery store, liquor bank, gas station). The estimated risk infection touching contaminated was low (less than 5 10,000) by quantitative microbial assessment, suggesting fomites play minimal role transmission. weekly percentage (out n = 33 unique per week) best predicted variation city-level cases with 7-day lead time. RNA on may be useful provide early warning case trends.

Язык: Английский

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SARS-CoV-2 wastewater surveillance data can predict hospitalizations and ICU admissions

The Science of The Total Environment, Год журнала: 2021, Номер 804, С. 150151 - 150151

Опубликована: Сен. 7, 2021

Язык: Английский

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