Analytical Chemistry,
Год журнала:
2024,
Номер
96(42), С. 16926 - 16936
Опубликована: Окт. 9, 2024
Protein-selection
mass
spectrometry
is
cost-effective
for
the
discovery
of
drugs
and
toxics.
Nuclear
receptors
(NRs)
are
major
targets
pharmaceuticals
endocrine-disrupting
chemicals
are,
thus,
widely
used
as
"bait"
proteins.
However,
their
application
limited
due
to
tendency
lose
protein
activity
during
cold
storage.
To
address
this
problem,
we
introduced
a
novel
biomineralization-based
approach
preserve
in
NRs,
exemplified
by
human
retinoic
acid
receptor
alpha
(hRARα),
target
cancer
leucocythemia
therapy.
Since
information
on
coordination
chemistry
metal
ion
NR
complexes
almost
unavailable,
applied
peptide
mapping
analysis
first
time
rational
design
his-hRARα-Co
phosphate
nanobiomaterial
with
high
bioactivity.
This
successfully
captured
hRARα
bioactive
from
Chinese
herb
environmental
water
discovered
an
unsaturated
fatty
acid,
(±)-(9
Medicinal Research Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
ABSTRACT
Proteins
hold
pivotal
importance
since
many
diseases
manifest
changes
in
protein
activity.
Proteomics
techniques
provide
a
comprehensive
exploration
of
structure,
abundance,
and
function
biological
samples,
enabling
the
holistic
characterization
overall
organisms.
Nowadays,
breadth
emerging
methodologies
proteomics
is
unprecedentedly
vast,
with
constant
optimization
technologies
sample
processing,
data
collection,
analysis,
its
scope
application
steadily
transitioning
from
bench
to
clinic.
Here,
we
offer
an
insightful
review
technical
developments
applications
biomedicine
over
past
5
years.
We
focus
on
profound
contributions
profiling
disease
spectra,
discovering
new
biomarkers,
identifying
promising
drug
targets,
deciphering
alterations
conformation,
unearthing
protein–protein
interactions.
Moreover,
summarize
cutting‐edge
potential
breakthroughs
pipeline
principal
challenges
proteomics.
Based
these,
aspire
broaden
applicability
inspire
researchers
enhance
our
understanding
complex
systems
by
utilizing
such
techniques.
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(26), С. 9722 - 9731
Опубликована: Июнь 23, 2023
As
typical
persistent
organic
pollutants,
polybrominated
diphenyl
ethers
(PBDEs)
tend
to
accumulate
in
edible
parts
of
rice,
posing
great
ecological
and
health
risks.
The
translocation
PBDEs
from
underground
aboveground
rice
is
a
crucial
procedure
determine
the
final
bioaccumulation
level.
Herein,
this
study
aimed
identify
transporter
proteins
for
plants
order
strengthen
our
understanding
mechanism
potential
prevention
strategy
PBDE
risk.
Similar
time-dependent
patterns
were
observed
among
root-to-shoot
factors
(TFs)
PBDEs,
expression
lysine
histidine
(LHT)
protein,
relative
levels
LHT
substrates
(phenylalanine
or
tyrosine),
implying
co-transport
phenylalanine,
tyrosine
by
carrier
LHT.
Fluorescence
spectra
circular
dichroism
showed
that
congeners
interfered
with
via
static
fluorescence
quenching
changes
protein's
secondary
structure.
vitro
sorption
fraction
as
revealed
equilibrium
analysis,
was
comparable
vivo
TF
values.
Knockout
OsLHT1
using
CRISPR/Cas9
technology
caused
48.2–78.4%
decrease
translocation.
Molecular
docking
simulation
suggested
inserted
into
same
ligand-binding
cavity
LHT,
substantiating
role
conformational
perspective.
Quantitative
structure
activity
relationship
analysis
demonstrated
ether-bond
oxygen
carbons
at
site
4
4′
molecules
are
significant
determinants
binding
affinity
protein
PBDEs.
In
summary,
discovered
acts
cellular
offered
comprehensive
molecular
explanation
plants,
covering
both
biological
chemical
perspectives.
These
findings
fill
knowledge
gap
on
endogenous
exogenous
pollutants.
Environmental Science Advances,
Год журнала:
2024,
Номер
3(5), С. 661 - 675
Опубликована: Янв. 1, 2024
Many
contaminants
can
have
long-term
effects
on
organisms
when
they
are
exposed
to
low
concentrations
for
extended
periods.
This
review
presents
new
methods
identifying
the
of
chemical
mixtures.
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(32), С. 11913 - 11925
Опубликована: Авг. 1, 2023
Chemical
contaminants
can
cause
adverse
effects
by
binding
to
the
liver-fatty
acid
protein
(L-FABP)
and
peroxisome
proliferator-activated
nuclear
receptor
γ
(PPARγ),
which
are
vital
in
lipid
metabolism.
However,
presence
of
numerous
compounds
environment
has
hindered
identification
their
ligands,
thus
only
a
small
portion
have
been
discovered
date.
In
this
study,
Affinity
Purification
with
Nontargeted
Analysis
(APNA)
was
employed
identify
ligands
L-FABP
PPARγ
indoor
dust
sewage
sludge.
A
total
83
nonredundant
features
were
pulled-out
His-tagged
as
putative
among
13
assigned
fatty
acids
hydrocarbon
surfactants.
contrast,
six
isolated
when
LBD
used
bait.
The
surfactants
confirmed
using
both
recombinant
proteins
reporter
cells.
These
surfactants,
along
>50
homologues
isomers,
detected
sludge
at
high
concentrations.
Fatty
explained
majority
(57.7
±
32.9%)
(66.0
27.1%)
activities
This
study
revealed
predominant
synthetic
PPARγ,
highlighting
importance
re-evaluating
chemical
safety.
Environmental Science & Technology,
Год журнала:
2024,
Номер
58(27), С. 11935 - 11944
Опубликована: Июнь 24, 2024
Pollutants
in
human
milk
are
critical
for
evaluating
maternal
internal
exposure
and
infant
external
exposure.
However,
most
studies
have
focused
on
a
limited
range
of
pollutants.
Here,
15
pooled
samples
(prepared
from
467
individual
samples)
three
areas
the
Yangtze
River
Delta
(YRD)
China
were
analyzed
by
gas
chromatography
quadrupole
time-of-flight
mass
spectrometry.
In
total,
171
compounds
nine
types
preliminarily
identified.
Among
these,
16
compounds,
including
2,5-di-tert-butylhydroquinone
2-tert-butyl-1,4-benzoquinone,
detected
first
time.
Partial
least-squares
discriminant
analysis
identified
ten
area-specific
pollutants,
2-naphthylamine,
9-fluorenone,
2-isopropylthianthrone,
benzo[a]pyrene,
among
Shanghai
(n
=
3),
Jiangsu
Province
6),
Zhejiang
6).
Risk
index
(RI)
values
calculated
indicated
that
legacy
polycyclic
aromatic
hydrocarbons
(PAHs)
contributed
only
20%
total
RIs
PAHs
derivatives,
indicating
more
attention
should
be
paid
to
with
various
functional
groups.
Nine
priority
pollutants
YRD
The
important
4-tert-amylphenol,
caffeine,
2,6-di-tert-butyl-p-benzoquinone,
which
associated
apoptosis,
oxidative
stress,
other
health
hazards.
results
improve
our
ability
assess
risks
posed
milk.
