Basrah Researches Sciences,
Год журнала:
2024,
Номер
50(2), С. 86 - 98
Опубликована: Дек. 31, 2024
The
emergence
of
such
widespread
pharmaceuticals
as
a
pollutant
has
become
one
the
world's
critical
environmental
problems
that
may
lead
to
both
public's
health
and
biodiversity
deterioration.
This
article
provides
an
exhaustive
account
current
understanding
persistence
pharmaceutical
contaminants
following
in-depth
analysis
additive
effects
existing
natural
biodegradation
pathways
on
human
impact
these
drugs.
Paying
special
attention
decomposing
agents
bacteria,
fungi,
algae
paper
estimates
their
ability
convert
drug
ingredients
compound
is
eventually
less
toxic.
Although
biologic
systems
contain
enormous
potential
for
killing
unwanted
pollutants,
variability
in
complexity
endurance
substances
overburden
degradation
capabilities
organisms
thus
necessitating
improved
methods.
Addressing
above-mentioned
factors,
which
include
temperature,
pH,
occurrence
other
contaminants,
play
crucial
role
have
direct
process
biodegradation,
enhancing
pollutants
removal
rate.
To
sum
up,
this
enables
science,
microbiology
bioengineering
creating
progressively
more
functional
sustainable
techniques
neutralizing
long-standing
toxins;
thus,
protecting
ecosystems,
well
health.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(10)
Опубликована: Фев. 26, 2024
Metformin
is
the
first-line
treatment
for
type
II
diabetes
patients
and
a
pervasive
pollutant
with
more
than
180
million
kg
ingested
globally
entering
wastewater.
The
drug's
direct
mode
of
action
currently
unknown
but
linked
to
effects
on
gut
microbiomes
may
involve
specific
microbial
reactions
drug.
In
wastewater
plants,
metformin
known
be
transformed
by
microbes
guanylurea,
although
genes
encoding
this
metabolism
had
not
been
elucidated.
present
study,
we
revealed
function
two
responsible
decomposition
(
The Journal of General Physiology,
Год журнала:
2024,
Номер
156(3)
Опубликована: Янв. 31, 2024
Proteins
from
the
small
multidrug
resistance
(SMR)
family
are
frequently
associated
with
horizontally
transferred
gene
arrays
found
in
bacteria
wastewater
and
human-adjacent
biosphere.
Recent
studies
suggest
that
a
subset
of
SMR
transporters
might
participate
metabolism
common
pharmaceutical
metformin
by
bacterial
consortia.
Here,
we
show
both
genomic
plasmid-associated
SMRGdx
functional
subtype
export
byproducts
microbial
metabolism,
particularly
high
efficiency
for
guanylurea.
We
use
solid-supported
membrane
electrophysiology
to
evaluate
transport
kinetics
guanylurea
native
substrate
guanidinium
four
representative
homologs.
Using
an
internal
reference
normalize
independent
experiments,
rates
comparable
homologs,
using
proteoliposome-based
assay,
2
proton:1
stoichiometry
is
maintained.
Additional
characterization
properties
focuses
on
structurally
characterized
homolog,
Gdx-Clo,
which
examined
pH
dependence
thermodynamics
binding
solved
x-ray
crystal
structure
bound.
Together,
these
experiments
contribute
two
main
ways.
By
providing
first
detailed
kinetic
examination
homolog
they
provide
framework
will
inform
future
mechanistic
this
model
protein.
Second,
study
casts
light
potential
role
handling
its
metabolic
byproducts,
insight
into
how
physiologies
co-opted
contend
new
selective
pressures.
iScience,
Год журнала:
2024,
Номер
27(2), С. 108900 - 108900
Опубликована: Янв. 12, 2024
Metformin
is
the
first-line
treatment
for
type
2
diabetes,
yet
its
mechanism
of
action
not
fully
understood.
Recent
studies
suggest
metformin's
interactions
with
gut
microbiota
are
responsible
exerting
therapeutic
effects.
In
this
study,
we
report
that
metformin
targets
microbial
enzyme
agmatinase,
as
a
competitive
inhibitor,
which
may
impair
agmatine
catabolism.
The
inhibition
constant
(K
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 20, 2024
The
biguanide
drug
metformin
is
a
first-line
blood
glucose-lowering
medication
for
type
2
diabetes,
leading
to
its
presence
in
the
global
environment.
However,
little
known
about
fate
of
by
microbial
catabolism.
Here,
we
characterize
Ni
Applied and Environmental Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 5, 2025
The
dioxygenase
TcsAB
is
a
specific
involved
in
the
initial
biodegradation
of
broad-spectrum
antibacterial
agent
triclosan
(TCS).
However,
it
exhibits
significantly
reduced
activity
under
cold
conditions.
In
this
study,
computer-directed
approach
combining
loop
engineering
and
N-terminal
truncation
was
utilized
to
decrease
thermostability
TcsAB,
thereby
enhancing
its
catalytic
environments.
iterative
mutant
(TcsAY277P/F279P/S311W/A313WTcsBN-terminal
truncation)
exhibited
2.54-fold
greater
efficiency
than
wild
type
at
15°C.
Molecular
dynamics
simulations
showed
that
mutations
introduced
substrate-binding
pocket
increased
flexibility,
leading
enhanced
through
binding
more
advantageous
conformation.
This
modified
employed
as
biological
component,
Pseudomonas
knackmussii
B13
used
chassis
cell
construct
an
engineered
strain
for
efficient
degradation
TCS
low
temperatures.
objective
enhance
capacity
bioremediation
natural
Insights
gained
from
study
may
inform
rational
redesign
enzymes
related
robustness
emerging
contaminants.IMPORTANCEThe
presence
surface
water
wastewater
poses
significant
risk
aquatic
organisms
human
health
due
high
resistance
degradation.
pollution
environment
metabolic
processes
microorganisms
represents
effective
remediation
strategy.
only
enzyme
has
been
identified
responsible
TCS.
Nevertheless,
markedly
diminished
actual
ambient
temperature
frequently
lower
optimum
reaction,
maintaining
30°C
reaction
condition
results
costs
energy
consumption
removal.
Accordingly,
low-temperature
will
facilitate
realistic
removal
aqueous
environment.
