Review article: Biodegradation of Pharmaceutical Pollutants: Challenges, Mechanisms, and Environmental Implications DOI Creative Commons

Wafaa Abdul-Rahman Alobaidi,

Ayat Abd-Aljaleel Nsaif,

Nooralhuda Aljawhar

и другие.

Basrah Researches Sciences, Год журнала: 2024, Номер 50(2), С. 86 - 98

Опубликована: Дек. 31, 2024

The emergence of such widespread pharmaceuticals as a pollutant has become one the world's critical environmental problems that may lead to both public's health and biodiversity deterioration. This article provides an exhaustive account current understanding persistence pharmaceutical contaminants following in-depth analysis additive effects existing natural biodegradation pathways on human impact these drugs. Paying special attention decomposing agents bacteria, fungi, algae paper estimates their ability convert drug ingredients compound is eventually less toxic. Although biologic systems contain enormous potential for killing unwanted pollutants, variability in complexity endurance substances overburden degradation capabilities organisms thus necessitating improved methods. Addressing above-mentioned factors, which include temperature, pH, occurrence other contaminants, play crucial role have direct process biodegradation, enhancing pollutants removal rate. To sum up, this enables science, microbiology bioengineering creating progressively more functional sustainable techniques neutralizing long-standing toxins; thus, protecting ecosystems, well health.

Язык: Английский

The universal accumulation of p-aminophenol during the microbial degradation of analgesic and antipyretic acetaminophen in WWTPs: a novel metagenomic perspective DOI Creative Commons
Chaofan Yin, Piaopiao Pan, Tao Li

и другие.

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Март 7, 2025

Abstract Background Acetaminophen, a widely used analgesic and antipyretic drug, has become significant aquatic micro-pollutant due to its extensive global production increased consumption, particularly during the COVID-19 pandemic. Its high-water solubility leads pervasive presence in wastewater treatment plants (WWTPs), posing substantial risks environment human health. Biological is one of promising approaches remove such pollutants. Although previous studies have isolated acetaminophen-degrading pure cultures proposed catabolic pathways, interactions between microbiotas acetaminophen, distribution feature acetaminophen degradation genes, gene-driven fate real-world remain largely unexplored. Results Among water samples from 20 WWTPs across China, was detected 19 at concentrations ranging 0.06 29.20 nM. However, p -aminophenol, more toxic metabolite, all significantly higher (23.93 108.68 nM), indicating bottleneck WWTPs. Metagenomic analysis both above datasets revealed consistently abundance initial amidases compared downstream enzymes, potentially having explained reason for bottleneck. Meanwhile, close correlation Actinomycetota by genome-based taxonomy suggests species-dependent pattern. Additionally, distinct amidase ApaA characterized newly Rhodococcus sp. NyZ502 (Actinomycetota), represents predominant category Significant phylogenetic structural diversity observed among putative suggest versatile hydrolysis potential Conclusions This study enhances our understanding acetaminophen’s environmental highlights possible occurrence ecological driven imbalanced genes process

Язык: Английский

Процитировано

0
Unveiling the Characteristics of Microbiota in Different Mucosal Layers of Leopard Coral Grouper (Plectropomus leopardus) DOI
Jiahui Wang, Shengtao Gao,

Zhenyuan Xu

и другие.

Marine Biotechnology, Год журнала: 2025, Номер 27(3)

Опубликована: Май 20, 2025

Язык: Английский

Процитировано

0
Microbial degradation of contaminants of emerging concern: metabolic, genetic and omics insights for enhanced bioremediation DOI Creative Commons

Bhavik A. Shah,

Harshit Malhotra,

Sandesh E. Papade

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Сен. 19, 2024

The perpetual release of natural/synthetic pollutants into the environment poses major risks to ecological balance and human health. Amongst these, contaminants emerging concern (CECs) are characterized by their recent introduction/detection in various niches, thereby causing significant hazards necessitating removal. Pharmaceuticals, plasticizers, cyanotoxins pesticides groups CECs that highly toxic found occur compartments biosphere. sources these compounds can be multipartite including industrial discharge, improper disposal, excretion unmetabolized residues, eutrophication etc ., while fate persistence determined factors such as physico-chemical properties, environmental conditions, biodegradability hydrological factors. resultant exposure microbiota has imposed a selection pressure resulted evolution metabolic pathways for biotransformation and/or utilization sole source carbon energy. Such microbial degradation phenotype exploited clean-up from environment, offering cost-effective eco-friendly alternative abiotic methods removal, mitigating toxicity. However, efficient bioprocess development bioremediation strategies requires extensive understanding individual components pathway gene clusters, proteins/enzymes, metabolites associated regulatory mechanisms. “Omics” “Meta-omics” techniques aid providing crucial insights complex interactions functions well community, enabling more effective targeted bioremediation. Aside natural isolates, engineering approaches employ application genetic enhance diversity rates. integration omics data will further developing systemic-level strategies, optimising process. This review describes bacterial catabolic pathways, genetics, four CECs: pharmaceuticals, cyanotoxins, pesticides.

Язык: Английский

Процитировано

3
Metformin hydrolase is a recently evolved, nickel-dependent, heteromeric ureohydrolase DOI Creative Commons

Jörg S. Hartig,

Malte Sinn,

Lea Riede

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Фев. 22, 2024

Abstract The anti-diabetic drug metformin is one of the most widely prescribed medicines in world. Together with its degradation product guanylurea, it a major pharmaceutical pollutant wastewater treatment plants and surface waters. An operon comprising two genes ureohydrolase family Pseudomonas Aminobacter bacteria has recently been implicated degradation. However, corresponding proteins have not characterized. Here we show that these encode Ni2+-dependent enzyme efficiently specifically hydrolyzed to guanylurea dimethylamine. active heteromeric complex α- β- subunits which only α-subunits contain conserved His Asp residues for coordination Ni2+ ions site. A crystal structure hydrolase revealed an α2β4 stoichiometry hexameric complex, unprecedented family. By studying closely related but more distributed enzyme, found putative predecessor dimethylguanidine instead metformin. Our findings establish molecular basis hydrolysis as primary pathway biodegradation provide insight into recent evolution response anthropogenic compound.

Язык: Английский

Процитировано

2
Combined effects of micropollutants and their degradation on prokaryotic communities at the sediment–water interface DOI Creative Commons

Adrien Borreca,

Stéphane Vuilleumier, Gwenaël Imfeld

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 22, 2024

Pesticides and pharmaceuticals enter aquatic ecosystems as complex mixtures. Various processes govern their dissipation effect on the sediment surface waters. These micropollutants often show persistence can adversely affect microorganisms even at low concentrations. We investigated effects procaryotic communities of metformin (antidiabetic drug), metolachlor (agricultural herbicide), terbutryn (herbicide in building materials). contaminants were introduced individually or a mixture (17.6 µM per micropollutant) into laboratory microcosms mimicking sediment-water interface. Metformin completely dissipated within 70 days, whereas persisted. Dissipation did not differ whether part mixture. Sequence analysis 16S rRNA gene amplicons evidenced distinct responses prokaryotic both water. Prokaryotic community variations mainly driven by matrix composition incubation time. Micropollutant exposure played secondary but influential role, with pronounced recalcitrant micropollutant Antagonistic synergistic non-additive identified for specific taxa across taxonomic levels response to This study underscores importance considering diversity interactions between micropollutants, communities, respective environments when examining interfaces affected multiple contaminants.

Язык: Английский

Процитировано

2
Metformin hydrolase is a recently evolved nickel-dependent heteromeric ureohydrolase DOI Creative Commons
Malte Sinn,

Lea Riede,

Jennifer R. Fleming

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 13, 2024

Abstract The anti-diabetic drug metformin is one of the most widely prescribed medicines in world. Together with its degradation product guanylurea, it a major pharmaceutical pollutant wastewater treatment plants and surface waters. An operon comprising two genes ureohydrolase family Pseudomonas Aminobacter species has recently been implicated degradation. However, corresponding proteins have not characterized. Here we show that these encode Ni 2+ -dependent enzyme efficiently specifically hydrolyzes to guanylurea dimethylamine. active heteromeric complex α- β- subunits which only α-subunits contain conserved His Asp residues for coordination ions site. A crystal structure hydrolase reveals an α 2 β 4 stoichiometry hexameric complex, unprecedented family. By studying closely related but more distributed enzyme, find putative predecessor dimethylguanidine instead metformin. Our findings establish molecular basis hydrolysis as primary pathway biodegradation provide insight into recent evolution response anthropogenic compound.

Язык: Английский

Процитировано

2
Co-occurrence, toxicity, and biotransformation pathways of metformin and its intermediate product guanylurea: Current state and future prospects for enhanced biodegradation strategy DOI
Lei Dong, Shuai Li, Jie Huang

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 921, С. 171108 - 171108

Опубликована: Фев. 22, 2024

Язык: Английский

Процитировано

1
Biochemical and genomic evidence for converging metabolic routes of metformin and biguanide breakdown in environmental Pseudomonads DOI Creative Commons

Katie B. Wissbroecker,

Anthony J. Zmuda,

Harsheeth Karumanchi

и другие.

Journal of Biological Chemistry, Год журнала: 2024, Номер unknown, С. 107935 - 107935

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

1
Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance family DOI Creative Commons
Rachael M. Lucero, Kemal Demirer, Trevor Justin Yeh

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 14, 2023

Proteins from the Small Multidrug Resistance (SMR) family are frequently associated with horizontally transferred multidrug resistance gene arrays found in bacteria wastewater and human-adjacent biosphere. Recent studies suggest that a subset of SMR transporters might participate metabolism common pharmaceutical metformin by bacterial consortia. Here, we show both genomic plasmid-associated

Язык: Английский

Процитировано

1
Discovery of a unique Ni2+-dependent heterohexameric metformin hydrolase DOI Creative Commons
Ning‐Yi Zhou, Tao Li,

Zhi-Jing Xu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Янв. 31, 2024

Abstract The biguanide drug metformin is prescribed worldwide as a first-line blood glucose-lowering medication for type 2 diabetes, leading to its presence in the environment around world. Despite mounting evidence indicating that metabolic interactions of with environmental or intestinal microbes affect ecological and human health, little known about fate by microbial catabolism. Here, we characterized Ni2+-dependent bacterial enzyme catalyzes hydrolysis form guanylurea dimethylamine. hydrolase MetCaCb heterohexamer an uneven α2β4 stoichiometry. Both subunits are from arginase protein family, members which typically homomultimers. Either subunit alone catalytically inactive, but together they work active highly specific metformin. crystal structure complex clearly shows coordination binuclear metal cluster only MetCa, following geometry typical family enzymes. A unique pseudoenzyme MetCb, evolved without cluster, contributes activity binder cognate. An in-silico search functional assay led discovery group MetCaCb-like pairs exhibiting environment. Our findings not establish genetic biochemical foundation catabolism also provide new insights into adaption ancient proteins toward newly occurred substrate.

Язык: Английский

Процитировано

0