ACS ES&T Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 8, 2024
Thiosulfate-assisted
anaerobic
fermentation
(AF)
effectively
converts
waste
activated
sludge
into
high-value
products
(e.g.,
short-chain
fatty
acids
(SCFAs)).
However,
the
roles
of
thiosulfate
in
organics
transformation,
electron
transfer,
and
microbial
interactions
within
AF
systems
are
not
fully
understood,
especially
under
long-term
operations.
In
this
study,
an
88
day
experiment
was
conducted
to
address
knowledge
gap.
The
results
indicated
average
SCFA
yield
3625.1
mg
COD/L
acetate
proportion
49.4%
with
a
dosage
600
S/L.
Model
organic
degradation
tests
revealed
that
functioned
as
acceptor,
facilitating
NAD+/NADH
stimulating
expression
protein
complexes
like
cytochrome
c
enhance
transport,
lowering
thermodynamic
barriers
propionate
butyrate
(ΔG1propionate
=
−335.0
kJ/mol;
ΔG2butyrate
−113.8
kJ/mol).
Molecular
ecological
networks
analysis
showed
strengthened
cooperative
relationships
among
biomarkers
hydrolytic
bacteria
(i.e.,
Proteiniphilum,
UBA5851),
acidogenic
UBA4179),
sulfur
reducers
JAEUSI01).
Functional
gene
using
random
forest
confirmed
upregulated
key
genes
2-oxoacid
ferredoxin
oxidoreductase)
associated
transfer
metabolism.
This
study
deepens
our
understanding
thiosulfate,
strengthening
cooperation
systems.
