Cell Response to Nanoplastics and Their Carrier Effects Tracked Real-Timely with Machine Learning-Driven Smart Surface-Enhanced Raman Spectroscopy Slides DOI
Ruili Li,

Shu-Ting Huang,

Yuyang Hu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Research on nanoplastic (NP) toxicity and their "carrier effects" human health remains nascent, especially for real-time, in situ monitoring of metabolic reactions live cells. Herein, we developed smart surface-enhanced Raman spectroscopy (SERS) slides using a cyclic centrifugation-enhanced electrostatic loading (CCEL) method to facilitatively track live-cell signals. The designed core-shell polystyrene NPs (mPS) with embedded probes successfully identified intracellular accumulation via distinct Raman-silent peak. SERS slide effectively monitored the changes induced by mPS at molecular level, distinguishing different stages membrane interaction, endocytosis process, endosomal aggregation, cell apoptosis. Besides, this platform was employed perform comparison cycle alterations bare effects", revealing that extended both S G2 phases BEAS-2B cells, while further prolonged disrupted S-phase progression. Additionally, integrated machine learning algorithms accurately predict impacts associated effects". This study provides label-free, situ, real-time NP-induced laying groundwork investigation into cytotoxic behaviors strategies mitigate NP toxicity.

Язык: Английский

Cell Response to Nanoplastics and Their Carrier Effects Tracked Real-Timely with Machine Learning-Driven Smart Surface-Enhanced Raman Spectroscopy Slides DOI
Ruili Li,

Shu-Ting Huang,

Yuyang Hu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Research on nanoplastic (NP) toxicity and their "carrier effects" human health remains nascent, especially for real-time, in situ monitoring of metabolic reactions live cells. Herein, we developed smart surface-enhanced Raman spectroscopy (SERS) slides using a cyclic centrifugation-enhanced electrostatic loading (CCEL) method to facilitatively track live-cell signals. The designed core-shell polystyrene NPs (mPS) with embedded probes successfully identified intracellular accumulation via distinct Raman-silent peak. SERS slide effectively monitored the changes induced by mPS at molecular level, distinguishing different stages membrane interaction, endocytosis process, endosomal aggregation, cell apoptosis. Besides, this platform was employed perform comparison cycle alterations bare effects", revealing that extended both S G2 phases BEAS-2B cells, while further prolonged disrupted S-phase progression. Additionally, integrated machine learning algorithms accurately predict impacts associated effects". This study provides label-free, situ, real-time NP-induced laying groundwork investigation into cytotoxic behaviors strategies mitigate NP toxicity.

Язык: Английский

Процитировано

0