Plastic-Degrading Microbial Consortia from a Wastewater Treatment Plant DOI Open Access
Andrea Salini, Luca Zuliani,

Paolo Matteo Gonnelli

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12747 - 12747

Опубликована: Ноя. 27, 2024

Plastic waste pollution has become a global crisis, with millions of tons plastic expected to accumulate in landfills and natural environments, posing serious threat wildlife human health. As current recycling methods remain inefficient, there is an urgent need for innovative enzymatic solutions break down plastics enable circular economy approach. In this study, we explore the plastic-degrading potential microorganisms enriched from activated sludge (AS) sourced municipal wastewater treatment plant (WWTP)—a known microplastic-contaminated industrial niche. Five microbial consortia (i.e., microbiomes) were under selective pressure using low-carbon conditions high concentrations polyester polymers, including post-consumer PET, PLA, virgin PLA. Enrichment was performed 100 days at 37 °C 50 °C, followed by microbiomes isolation metagenomic analysis identify plastic-active bacteria their enzymes. The results revealed that PLA but not effectively degraded microbiomes, as confirmed nuclear magnetic resonance (NMR) gel permeation chromatography (GPC), showing significant molecular weight reduction compared abiotic controls. Microbial community highlighted distinct enrichment profile driven polymer composition temperature. At Bacillales order became predominant population, whereas more diverse within Proteobacteria Actinobacteria phyla selected. Nonetheless, communities both temperatures included members degradation. Moreover, putative PET/PLA hydrolases also observed. These findings suggest AS are reservoir polyester-active enzymes, particularly PLA-depolymerases, hold promise advancing biotechnological strategies mitigate through re- up-cycling.

Язык: Английский

Accelerating biodegradation efficiency of low-density polyethylene and its hazardous dissolved organic matter using unexplored polyolefin-respiring bacteria: New insights on degradation characterization, biomolecule influence and biotransformation pathways DOI

Maseed Uddin,

Swathi Krishnan Venkatesan,

Subhan Kumar Pal

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138144 - 138144

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Microplastics as persistent and vectors of other threats in the marine environment: Toxicological impacts, management and strategical roadmap to end plastic pollution DOI Creative Commons
Sameh S. Ali, Mohammed Hussein M. Alsharbaty,

Rania Al-Tohamy

и другие.

Environmental Chemistry and Ecotoxicology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

5
Quantification of the Uptake and Biodistribution of Nanoplastics in Escherichia coli DOI
Yan Gao,

Quanzhi Xiao,

Jie Zhang

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Nanoplastics (NPs) are prevalent in the environment, posing risks to ecosystems and human health. While research into their effects on bacterial activity has increased, mechanisms underlying NP-bacteria interactions─specifically whether NPs penetrate cells or adhere cell surface─remain poorly understood. This knowledge gap largely stems from absence of quantitative analytical methods. Herein, we developed a novel approach combining lysozyme treatment with pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) differentiate between intracellular wall-bound Escherichia coli (E. coli) quantitatively. The method involves selective removal wall using lysozyme, protein corona-induced extraction enrich NPs, hydrogen peroxide digestion eliminate protoplast interference before Py-GC/MS analysis. Validation europium (Eu)-labeled quantified by inductively coupled plasma mass (ICP-MS), confirmed method's accuracy reliability. Using this approach, found that after NP exposure, only small fraction (9.6-10.5%) penetrated E. cells, while majority (36.9-63.8%) adhered surface. Transmission electron microscopy further corroborated these findings. Consequently, work provides robust tool for quantification uptake biodistribution systems, advancing our understanding NP-microorganism interactions environmental implications.

Язык: Английский

Процитировано

0
Microplastic Abundance, Characteristics, and Associated Ecological Risk Assessment in the Selected Coastal Areas of Baybay City, Leyte, Philippines DOI

Abu Sayed Al Helal,

AB Siddique,

Teofanes Patindol

и другие.

Regional Studies in Marine Science, Год журнала: 2025, Номер unknown, С. 104246 - 104246

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Standardization guidelines and future trends for PET hydrolase research DOI Creative Commons
Ren Wei, Peter Westh, Gert Weber

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Май 20, 2025

Enzymatic depolymerization of polyethylene terephthalate (PET) towards monomer recycling offers a green route to circular plastic economy, with scale-up currently underway. Yet, inconsistent assessment methods hinder clear comparisons between various PET hydrolases. This Perspective aims identify critical gaps in this dynamic research field and outline key principles for selecting tailoring novel enzymes, such as using uniform samples standardizing reaction settings that mimic industrial conditions. Applying these guidelines will improve enzyme screening efficiency, increase data reproducibility, deepen the understanding interfacial biocatalysis, ultimately accelerate development more robust cost-effective bio-based methods.

Язык: Английский

Процитировано

0
Lima Megacity’s Influence on Aquatic Microbial Communities in the Rímac River: Dominance Over Spatial and Seasonal Variations DOI Creative Commons

Tatyana Samaniego,

Renato La Torre,

Gisella Orjeda

и другие.

Microbial Ecology, Год журнала: 2025, Номер 88(1)

Опубликована: Май 31, 2025

Язык: Английский

Процитировано

0
Microbial and Enzymatic Biodegradation of Plastic Waste for a Circular Economy DOI Creative Commons
Muhammad Akram, Rangasamy Savitha, Gemma K. Kinsella

и другие.

Applied Sciences, Год журнала: 2024, Номер 14(24), С. 11942 - 11942

Опубликована: Дек. 20, 2024

Plastics play a crucial role in modern life, but their accumulation poses serious threat to both the environment and human health. Due effects on terrestrial aquatic environment, it is essential develop sustainable approaches dispose of waste plastics. Traditional methods plastic disposal, such as burning landfilling, are problematic since they produce hazardous byproducts. Biodegradation potentially effective, eco-friendly approach which uses microbial consortia or isolated enzymes break down waste. Enzymes interact with surfaces hydrolyse large polymer chains into smaller units. These byproducts can then be utilised carbon sources by microbes, eventually converted CO2 water. This review explores principal degradation, focus existing emerging polymers made readily biodegradable. In addition, valorisation for converting valuable considered. The implementation circular economy expected lead further development, including scaling up efficient bio-upcycling processes, serve stimulate environmental removal value-added use post-consumer streams.

Язык: Английский

Процитировано

2
Plastic-Degrading Microbial Consortia from a Wastewater Treatment Plant DOI Open Access
Andrea Salini, Luca Zuliani,

Paolo Matteo Gonnelli

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12747 - 12747

Опубликована: Ноя. 27, 2024

Plastic waste pollution has become a global crisis, with millions of tons plastic expected to accumulate in landfills and natural environments, posing serious threat wildlife human health. As current recycling methods remain inefficient, there is an urgent need for innovative enzymatic solutions break down plastics enable circular economy approach. In this study, we explore the plastic-degrading potential microorganisms enriched from activated sludge (AS) sourced municipal wastewater treatment plant (WWTP)—a known microplastic-contaminated industrial niche. Five microbial consortia (i.e., microbiomes) were under selective pressure using low-carbon conditions high concentrations polyester polymers, including post-consumer PET, PLA, virgin PLA. Enrichment was performed 100 days at 37 °C 50 °C, followed by microbiomes isolation metagenomic analysis identify plastic-active bacteria their enzymes. The results revealed that PLA but not effectively degraded microbiomes, as confirmed nuclear magnetic resonance (NMR) gel permeation chromatography (GPC), showing significant molecular weight reduction compared abiotic controls. Microbial community highlighted distinct enrichment profile driven polymer composition temperature. At Bacillales order became predominant population, whereas more diverse within Proteobacteria Actinobacteria phyla selected. Nonetheless, communities both temperatures included members degradation. Moreover, putative PET/PLA hydrolases also observed. These findings suggest AS are reservoir polyester-active enzymes, particularly PLA-depolymerases, hold promise advancing biotechnological strategies mitigate through re- up-cycling.

Язык: Английский

Процитировано

0