A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury

Food & Function, Год журнала: 2021, Номер 12(14), С. 6271 - 6282

Опубликована: Янв. 1, 2021

A polysaccharide from C. sinensis regulated the balance between Treg and Th17 cells, modulated microbiota composition in gut of mice with Cy-induced intestinal injury.

Язык: Английский

---

Sulfation modification enhances the intestinal regulation of Cyclocarya paliurus polysaccharides in cyclophosphamide-treated mice via restoring intestinal mucosal barrier function and modulating gut microbiota

Food & Function, Год журнала: 2021, Номер 12(24), С. 12278 - 12290

Опубликована: Янв. 1, 2021

This work aimed to investigate the effects of a sulfated derivative Cyclocarya paliurus polysaccharide (SCP3) on cyclophosphamide (CTX)-induced intestinal barrier damage and microbiota in mice. The results showed that SCP3 increased intestine antioxidant defense, repaired via restoring villi length crypt depth, up-regulated expression tight junction proteins. Bacterial 16S rRNA sequencing confirmed dramatically altered structure gut microbiota, diversity regulated relative abundances specific bacteria, including increasing Bacteroidetes, Firmicutes, Tenericutes, Oscillospira, Akkermansia, decreasing Proteobacteria Verrucomicrobia. In conclusion, can improve function CTX-treated mice enhancing oxidative stress capacity, repairing mucosal barrier, regulating microorganisms, this study provides scientific theoretical basis for application food pharmaceutical fields.

Язык: Английский

---

Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(10), С. 3344 - 3356

Опубликована: Март 1, 2022

Gut microbiota plays an important role in the regulation of food allergy. However, interactions between gut flora and immune system are not well studied. Here, we obtained ovalbumin (OVA)-sensitive BALB/c mice, combined with serum untargeted metabolomics to investigate mechanisms interactions. The results showed that 17 metabolites were downregulated, enriched aminoacyl-tRNA biosynthesis pathway, whereas indole-3-propionic acid (IPA) was increased. Six operational taxonomic units (OTUs) at family level altered correlated endpoints. Combined metabolomic microbiomic analyses revealed IPA levels differential bacterial OTUs a positive correlation Treg splenic lymphocytes. These suggest regulatory effects intestinal on allergic responses may be achieved by metabolizing tryptophan produce indole derivatives pathway. formation OVA tolerance mice related enrichment Peptostreptococcaceae, Ruminococcaceae, Lactobacillaceae.

Язык: Английский

---

The regulating effect of Tibet Opuntia ficus-indica (Linn.) Mill. polysaccharides on the intestinal flora of cyclophosphamide-induced immunocompromised mice

International Journal of Biological Macromolecules, Год журнала: 2022, Номер 207, С. 570 - 579

Опубликована: Март 12, 2022

Язык: Английский

---

Prebiotic effects of goji berry in protection against inflammatory bowel disease

Critical Reviews in Food Science and Nutrition, Год журнала: 2022, Номер 63(21), С. 5206 - 5230

Опубликована: Янв. 6, 2022

The prevalence of inflammatory bowel disease (IBD) is increasing, which concerning because IBD a known risk factor for the development colorectal cancer. Emerging evidence highlights environmental factors, particularly dietary factors and gut microbiota dysbiosis, as pivotal inducers onset. Goji berry, an ancient tonic food nutraceutical supplement, contains range phytochemicals such polysaccharides, carotenoids, polyphenols. Among these phytochemicals, L. barbarum polysaccharides (LBPs) are most important functional constituents, have protective effects against oxidative stress, inflammation, neurodegeneration. Recently, beneficial goji berry associated LBPs consumption were linked to prebiotic effects, can prevent dysbiosis with IBD. This review assessed pertinent literature on focusing their metabolites in mediating observed effects.

Язык: Английский

---

Combined effect of salinity and hypoxia on digestive enzymes and intestinal microbiota in the oyster Crassostrea hongkongensis

Environmental Pollution, Год журнала: 2023, Номер 331, С. 121921 - 121921

Опубликована: Май 30, 2023

Язык: Английский

---

Immunomodulatory activity of polysaccharides from the mycelium of Aspergillus cristatus, isolated from Fuzhuan brick tea, associated with the regulation of intestinal barrier function and gut microbiota

Food Research International, Год журнала: 2021, Номер 152, С. 110901 - 110901

Опубликована: Дек. 16, 2021

Язык: Английский

---

Mechanism of the Immunomodulatory Effect of the Combination of Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus on Immunocompromised Rats

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Июнь 15, 2021

Immunodeficiency is a very common condition in suboptimal health status and during the development or treatment of many diseases. Recently, probiotics have become an important means for immune regulation. The present study aimed to investigate mechanism immunomodulatory effect combination live Bifidobacterium , Lactobacillus Enterococcus Bacillus (CBLEB), which drug used by approximately 10 million patients every year, on cyclophosphamide-immunosuppressed rats. Cyclophosphamide (40 mg/kg) was intraperitoneally injected induce immunosuppression rat model days 1, 2, 3, 10. Starting from day 4, rats were continuously gavaged with CBLEB solution 15 days. samples collected determine routine blood test parameters, liver kidney functions, serum cytokine levels, gut microbiota, fecal metabolomes, transcriptomes, histopathological features. results indicated that reduced cyclophosphamide-induced death, weight loss, damage gut, liver, spleen, lungs eliminated increase mean hemoglobin content GGT, M-CSF, MIP-3α levels decrease red cell distribution width total protein creatinine blood. Additionally, corrected dysbiosis microbiota all alterations at phylum level feces, including enrichment Proteobacteria, Fusobacteriota, Actinobacteriota depletion Spirochaetota Cyanobacteria. Furthermore, alleviated whole metabolome profile, 1-heptadecanol, succinic acid, hexadecane-1,2-diol, nonadecanoic pentadecanoic acid benzenepropanoic hexane. also D-lyxose D-galactose, L-5-oxoproline, L-alanine, malic acid. transcriptome analysis related induction recovery cyclophosphamide-altered carbohydrate metabolism signal transduction. In conclusion, provides experimental basis comprehensive application immunodeficiency.

Язык: Английский

---

Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota‐Dependent Manner

Molecular Nutrition & Food Research, Год журнала: 2022, Номер 67(2)

Опубликована: Ноя. 24, 2022

Ulcerative colitis (UC) is a common chronic recurrent inflammatory bowel disease. This study attempts to reveal the improvement mechanism of floral mushroom polysaccharide (FMPS) on UC from perspective coordinated interaction between intestinal microbes and helper T cell 17 (Th17)/regulatory (Treg) balance.Dextran sulfate sodium (DSS)-induced mice model used for experiment. The results suggest that FMPS up-regulated expression occludin, ZO-1, MUC2, down-regulated secretion TNF-α, IL-1β, IL-6 in mice. Importantly, restores Th17/Treg balance. Meanwhile, can regulate microorganisms improve level short-chain fatty acids (SCFAs) Intestinal microbial depletion fecal microbiota transplantation (FMT) experiments ameliorated mediated by microbiome. Flow cytometry further proves balance microbial-dependent manner.These indicate has potential UC, its depends restoration microorganisms. Therefore, it suggested dietary supplement be potentially intervene UC.

Язык: Английский

---

The underlying mechanism of A‐type procyanidins from peanut skin on DSS ‐induced ulcerative colitis mice by regulating gut microbiota and metabolism

Journal of Food Biochemistry, Год журнала: 2022, Номер 46(7)

Опубликована: Фев. 25, 2022

Ulcerative colitis (UC) is a kind of inflammatory bowel disease. Procyanidins have been found to prevent UC. However, most research has focused on the alleviation effect B-type procyanidins UC and ignored those A-type procyanidins. Hence, this study aims investigate anti-UC potential mechanism by combining gut microbiome metabolic profile. was induced dextran sulfate sodium (DSS) in Balb/c mice, then mice were administrated with peanut skin (PSP; rich procyanidins) for 9 days. Administration PSP can ameliorate DSS-induced mediating intestinal barrier, expression cytokines (TNF-α, IL-β, IL-6, IL-10) oxidative stress (MDA, T-SOD, NO, iNOS) mice. We observed that affects microbiota colon metabolomic patterns The 16S rDNA sequencing showed increase abundance Lachnospiraceae_NK4A136_group, Oscillibacter Roseburia decrease Bacteroides, Helicobacter, Parabacteroides, Escherichia-Shigella, Enterobacter after treatment. tissue metabolome significantly altered, as reflected regulating taste transduction, mTOR signaling pathway, PI3K-Akt FoxO pathway improve protection against Practical applications investigated anti-ulcerative its (PSP). This suggests abundant may be an effective candidate dietary supplementation alleviate symptoms metabolism.

Язык: Английский

---