Amygdalin Alleviates DSS-Induced Colitis by Restricting Cell Death and Inflammatory Response, Maintaining the Intestinal Barrier, and Modulating Intestinal Flora

Cells, Год журнала: 2024, Номер 13(5), С. 444 - 444

Опубликована: Март 3, 2024

Inflammatory bowel disease (IBD) refers to a cluster of intractable gastrointestinal disorders with an undetermined etiology and lack effective therapeutic agents. Amygdalin (Amy) is glycoside extracted from the seeds apricot other Rosaceae plants it exhibits wide range pharmacological properties. Here, effects mechanisms Amy on colitis were examined via 16S rRNA sequencing, ELISA, transmission electron microscopy, Western blot, immunofluorescence. The results showed that administration remarkably attenuated signs (reduced body weight, increased activity index, shortened colon length) histopathological damage in dextran sodium sulfate (DSS)-challenged mice. Further studies revealed significantly diminished DSS-triggered gut barrier dysfunction by lowering pro-inflammatory mediator levels, inhibiting oxidative stress, reducing intestinal epithelial apoptosis ferroptosis. Notably, lowered TLR4 expression phosphorylation proteins related NF-κB MAPK pathways. Furthermore, modulated balance flora, including selective rise abundance S24-7 decline Allobaculum, Oscillospira, Bacteroides, Sutterella, Shigella. In conclusion, can alleviate colitis, which provides data support utility combating IBD.

Язык: Английский

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Regulation of gut microbiota-bile acids axis by probiotics in inflammatory bowel disease

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Сен. 23, 2022

Inflammatory bowel disease (IBD) is characterized by chronic and relapsing inflammation of gastrointestinal tract, with steadily increased incidence prevalence worldwide. Although the precise pathogenesis remains unclear, gut microbiota, bile acids (BAs), aberrant immune response play essential roles in development IBD. Lately, dysbiosis including certain decreased beneficial bacteria pathogens BAs metabolism have been reported The inhabited human critical functions BA biotransformation. Patients active IBD elevated primary conjugated secondary BAs, accompanied impaired transformation activities (mainly deconjugation 7α-dehydroxylation) microbiota. Probiotics exhibited positive effects different mechanisms therapy This review discussed effectiveness probiotics clinical animal model studies that might involve microbiota-BAs axis. More importantly, possible on regulating axis were elucidated, which we focused containing salt hydrolase or BA-inducible enzymes at genus/species level participate Furthermore, exerted activation BA-activated receptors intestinal immunity also summarized, partially explain protect Therefore, this will provide new insights into a better understanding targeting

Язык: Английский

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Effect of Bifidobacterium animalis subsp. lactis SF on enhancing the tumor suppression of irinotecan by regulating the intestinal flora

Pharmacological Research, Год журнала: 2022, Номер 184, С. 106406 - 106406

Опубликована: Авг. 18, 2022

Язык: Английский

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Sea Cucumber Peptides Ameliorate DSS-Induced Ulcerative Colitis: The Role of the Gut Microbiota, the Intestinal Barrier, and Macrophage Polarization

Nutrients, Год журнала: 2023, Номер 15(22), С. 4813 - 4813

Опубликована: Ноя. 17, 2023

The incidence of ulcerative colitis (UC) is increasing annually. There are few treatments for UC patients, and some drugs have serious side effects. Sea cucumber peptide (SCP) has anti-inflammatory, antioxidant other biological activities, various sea species in pharmaceutical development. However, relevant studies on the effects SCP progression still lacking. In this study, a mouse model acute was induced by 3% dextran sulfate (DSS), effect 500 mg/kg investigated. results showed that can alleviate DSS-induced colon damage intestinal barrier damage. significantly inhibited expression inflammatory factors oxidative stress mice. reversed microbiota dysregulation DSS, growth Sutterella, Prevotella_9 Escherichia-Shigella harmful bacteria, increased abundance Lachnospiraceae_NK4A136_group. At same time, treatment LPS-induced polarization M1 macrophages, which may be mediated two monopeptides, IPGAPGVP TGPIGPPGSP, via FPR2. conclusion, protect against modulating composition inhibiting macrophages.

Язык: Английский

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Complex probiotics alleviate ampicillin-induced antibiotic-associated diarrhea in mice

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Апрель 14, 2023

Antibiotic-associated diarrhea (AAD) is a common side effect during antibiotic treatment, which can cause dysbacteriosis of the gut microbiota. Previous studies have shown beneficial effects in AAD treatment with Bifidobacterium lactis XLTG11, Lactobacillus casei Zhang, plantarum CCFM8661, and rhamnosus Probio-M9. However, no been conducted on immunomodulatory protective intestinal barrier function four complex probiotics. The aim our study to investigate alleviation probiotics ampicillin-induced AAD.Thirty-six BALB/c mice were randomly divided into six groups: normal control group (NC), model (MC), low-, medium-, high-dose groups (LD, MD, HD), positive drug (Bifico, 1 × 107 cfu) (PDC; Bifico, also known as Triple Live Capsule, composed longum, acidophilus, Enterococcus faecalis). An was established by intragastric administration ampicillin, gavage different doses Bifico. weight gain, fecal water content, loose stool grade, permeability, total protein albumin levels, barrier, cytokine microbiota determined.The results showed that significantly decreased ileum tissue damage. Their application increased SIgA, TP, ALB levels. Additionally, levels pro-inflammatory cytokines those anti-inflammatory cytokines. Meanwhile, mRNA expression ZO-1, occludin, claudin-1, MUC2 upregulated probiotic-treated group. Furthermore, diversity modulated changes composition caused ampicillin. At phylum level, abundance Proteobacteria HD lower than MC group, whereas Bacteroidetes higher. genus abundances Klebsiella Parabacteroides lower, Bacteroides, Muribaculaceae, higher Moreover, Spearman's correlation analysis found several specific correlated AAD-related indicators.We improved diarrhea-related indexes, regulated diversity, barrier-related genes, preserved function, relieved inflammation injury, thereby effectively improving AAD-associated symptoms. Graphical Abstract.

Язык: Английский

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