Acupoint catgut embedding improves learning and memory impairment in vascular dementia rats

Annals of Translational Medicine, Год журнала: 2023, Номер 11(2), С. 108 - 108

Опубликована: Янв. 1, 2023

Vascular dementia (VD) is a disease that affects brain function through cerebrovascular disease. Due to its complex pathogenesis, there no effective drug treatment for VD. The present study aimed evaluate the role of acupoint catgut embedding in rats with VD and possible molecular mechanism.A modified 4 vessel occlusion (4-VO) method was used establish model rat, spatial learning memory ability assessed using Morris water maze (MWM) test. protein expression levels were detected by Western blot. Hematoxylin eosin (HE) staining histological analysis enzyme-linked immunosorbent assay (ELISA) applied serum inflammatory factors.We successfully constructed impairment, hippocampus injury, high response. Treatment significantly reduced escape latency increased time target quadrant platform crossing times. VD-mediated hippocampal tissue damage reaction [down-regulating interleukin-1β (IL-1β), interleukin-6 (IL-6)] alleviated treatment. In addition, further mechanism exploration found could improve activity toll-like receptor (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) signaling pathway. summary, improved loss, pathological hippocampus, inhibited inflammation response rats, which probably related inhibition TLR4/MyD88/NF-κB pathway.Acupoint may warrant as an adjuvant therapy

Язык: Английский

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Metabolomic Profiling and Biological Investigation of the Marine Sponge-Derived Fungus Aspergillus sp. SYPUF29 in Response to NO Condition

Journal of Fungi, Год журнала: 2024, Номер 10(9), С. 636 - 636

Опубликована: Сен. 5, 2024

Marine-derived fungi are assuming an increasingly central role in the search for natural leading compounds with unique chemical structures and diverse pharmacological properties. However, some gene clusters not expressed under laboratory conditions. In this study, we have found that a marine-derived fungus

Язык: Английский

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Protective effect of nanoparticle NLRP3 inhibitor (MCC950) on cranial nerves in rats with vascular cognitive impairment

Materials Express, Год журнала: 2023, Номер 13(6), С. 955 - 960

Опубликована: Июнь 1, 2023

Background : Chronic cerebral hypoperfusion (CCH) is a common cause of Vascular cognitive impairment (VCI). The neuroinflammation caused by ischemia play key role in the progression ailment process. over activation NLRP3/Caspase-1/IL-1 β signaling aggravates inflammatory response. We investigated whether NLRP3 inhibitors have protective effect on cranial nerves rats with VCI. Methods Male SD were selected arbitrarily and categorized into sham operation group, CCH group CCH+MCC950 (10 mg/kg). rat model was formed bilateral carotid artery occlusion (BCCAO). changes rat’s blood flow observed laser speckle imaging after operation. given intraperitoneal (i.p) injection normal saline, therapeutic i.p having dose MCC950 behavior analyzed through Y maze open field experiments, hematoxylin-eosin (HE) staining technique employed to analyze rats. histological hippocampus under following conditions, cell apoptosis Tune staining, protein expressions NLRP3, IL (interleukin)-1 , cleaved-Caspase-1 Western blot. Results treatment enhanced working memory rats, each showed no motor deficit. As shown HE inhibition reduced neuronal damage pyroptosis. In addition, diminishes appearance IL-1 brain tissue inhibiting inflammasome formation. Conclusions can improve learning ability vascular impairment, mechanism might be linked decreasing apoptosis.

Язык: Английский

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Characteristic early changes of Glu and Cho in brain regions affected by different types of subjective cognitive decline and their clinical significance

Medicine, Год журнала: 2023, Номер 102(49), С. e36457 - e36457

Опубликована: Дек. 8, 2023

To discuss the early changes of Glu and Cho in affected areas different types subjective cognitive decline, including amnestic MCI (aMCl), non-amnestic (naMCI) vascular impairment no dementia (VCIND), using Proton Magnetic Resonance Spectroscopy ( 1 H-MRS) technology. Routine head MRI l H-MRS examinations were performed on 50 clearly diagnosed aMCI patients, 44 naMCI VCIND elderly individuals with normal function. Measure volume patient bilateral hippocampus. Using hippocampus, left posterior cingulate gyrus (PCG), frontal lobe as regions interest, scope under peak N-acetylaspartate (NAA), choline complex (Cho), glutamate (Glu), Metabolic Images (mI), creatine (Cr) was tested. Perform a correlation analysis between NAA/Cho/Cr values group MoCA score. All experimental subjects right-handed. The NAACr both hippocampus greatly lower than those control P < .05). NAA/Cr sides correlated score (P LHp PCG groups NC showed obvious decrease compared to NC. Glu/Cr discrepancy > .05. In lobe, contrast NC, mI/Cr higher relative concentration 1H-MRS metabolites can indicate presence hippocampal neuronal damage before morphological occur reflect function patients certain extent. There are regional differences mI metabolism brain groups. provides an effective basis for clinical differentiation naMCI.

Язык: Английский

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