Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(14), С. 8237 - 8246
Опубликована: Март 26, 2024
Flavonols
represented
by
quercetin
have
been
widely
reported
to
biological
activities
of
regulating
lipid
metabolism.
However,
the
differences
in
flavonols
with
different
structures
lipid-lowering
activity
and
influencing
factors
remain
unclear.
In
this
study,
stability,
transmembrane
uptake
ratio,
metabolism
regulation
12
flavonol
compounds
3T3-L1
cell
model
were
systematically
compared.
The
results
showed
that
kaempferide
had
highest
cellular
ratio
most
potent
inhibitory
effect
on
adipogenesis
at
a
dosing
concentration
20
μM,
followed
isorhamnetin
kaempferol.
They
inhibited
TG
accumulation
more
than
65%
downregulated
expression
PPARγ
SREBP1c
60%.
other
four
aglycones,
including
quercetin,
did
not
exhibit
significant
due
structural
instability
culture
medium.
Meanwhile,
five
glucosides
quite
stable
but
low
no
obvious
was
observed.
Correlation
analysis
also
for
11
except
galangin,
positively
correlated
(p
<
0.05,
r
=
0.6349).
These
findings
may
provide
valuable
idea
insight
exploring
structure-based
flavonoids
level.
Antioxidants,
Год журнала:
2023,
Номер
12(11), С. 1939 - 1939
Опубликована: Окт. 31, 2023
With
the
development
of
an
aging
sociality,
aging-related
diseases,
such
as
Alzheimer's
disease,
cardiovascular
and
diabetes,
are
dramatically
increasing.
To
find
small
molecules
from
natural
products
that
can
prevent
human
beings
occurrence
these
we
used
lifespan
assay
yeast
a
bioassay
system
to
screen
antiaging
substance.
Isoquercitrin
(IQ),
substance,
was
isolated
Apocynum
venetum
L.,
herbal
tea
commonly
consumed
in
Xinjiang,
China.In
present
study,
utilized
molecular-biology
technology
clarify
mechanism
action
IQ.The
replicative
lifespans
K6001
yeasts
chronological
YOM36
were
confirm
effect
IQ.
Furthermore,
reactive
oxygen
species
(ROS)
malondialdehyde
(MDA)
assay,
survival
under
stresses,
real-time
polymerase
chain
reaction
(RT-PCR)
Western
blotting
analyses,
replicative-lifespan
mutants,
Δsod1,
Δsod2,
Δgpx,
Δcat,
Δskn7,
Δuth1,
Δatg32,
Δatg2,
Δrim15
K6001,
autophagy
flux
analysis,
after
giving
mitophagy
inhibitor
activator
performed.IQ
extended
yeasts.
nitrogen
(RNS)
showed
no
change
during
growth
phase
but
significantly
decreased
stationary
treatment
with
The
rates
oxidative-
thermal-stress
conditions
improved
upon
IQ
treatment,
thermal
stress
alleviated
by
increasing
superoxide
dismutase
(Sod)
activity.
Additionally,
ROS
MDA
while
activity
antioxidant
enzymes.
However,
it
could
not
prolong
Δuth1
K6001.
increased
induction,
presence
free
green
fluorescent
protein
(GFP)
cytoplasm,
ubiquitination
mitochondria
YOM38
at
level.
did
Δatg2
Δatg32
Moreover,
led
decrease
Sch9
level
increase
nuclear
translocation
Rim15
Msn2.These
results
indicated
Sch9/Rim15/Msn
signaling
pathway,
well
antioxidative
stress,
anti-thermal
autophagy,
involved
effects
Medicine,
Год журнала:
2024,
Номер
103(25), С. e38315 - e38315
Опубликована: Июнь 21, 2024
Gegensan
(GGS)
has
been
reported
for
the
treatment
of
alcoholic
liver
disease
(ALD),
but
its
therapeutic
mechanism
is
still
unclear.
This
paper
aims
to
determine
and
targets
action
GGS
on
utilizing
network
pharmacology
bioinformatics.
The
active
ingredients
in
were
screened
literature
databases,
common
ALD
then
obtained
from
public
databases
construct
diagram
traditional
Chinese
medicine-active
ingredient
targets.
Based
targets,
Gene
Ontology
enrichment
analysis
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
performed
find
target
pathways,
core
out
by
combining
differential
protein-protein
interaction
analysis.
Molecular
docking
was
verify
binding
effect
between
corresponding
ingredients.
have
84
91
After
subsequent
analysis,
10
identified.
KEGG
analyses
showed
that
main
BPs
included
response
lipopolysaccharide,
inflammatory
response,
etc.
pathways
involved
regulation
lipid-atherosclerosis
pathway
pathway,
Further
molecular
CYP1A1,
CYP1A2,
CXCL8,
ADH1C,
MMP1,
SERPINE1,
COL1A1,
APOB,
their
4
ingredients,
Naringenin,
Kaempferol,
Quercetin,
Stigmasterol,
a
greater
potential.
above
results
suggest
can
regulate
lipid
metabolism
process,
alleviate
accumulation
oxidative
stress
caused
ethanol.
study
analyzed
mechanisms
ALD,
which
provides
certain
theoretical
support
further
development
reference
research
ALD.
