Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 19, 2024
CRISPR/Cas12a-based
biosensors
have
garnered
significant
attention
in
the
field
of
point-of-care
testing
(POCT),
yet
majority
CRISPR-based
POCT
methods
employ
fluorescent
systems
as
report
probes.
Herein,
we
a
new
CRISPR/Cas12a-enabled
multicolor
visual
biosensing
strategy
for
rapid
detection
disease
biomarkers.
The
proposed
assay
provided
vivid
color
responses
to
enhance
accuracy
detection.
In
existence
target,
The Analyst,
Год журнала:
2024,
Номер
149(17), С. 4343 - 4350
Опубликована: Янв. 1, 2024
Food
is
a
necessary
source
of
energy,
but
it
also
serves
as
pathway
for
transmitting
infectious
pathogens,
making
food
safety
matter
great
concern.
Rapid,
accurate,
and
specific
detection
methods
foodborne
viruses
are
crucial.
Surface-Enhanced
Raman
Scattering
(SERS),
due
to
its
superior
sensitivity
characteristic
fingerprint
spectra,
holds
enormous
potential.
However,
the
limitations
SERS,
requires
conditions
achieve
specificity.
In
order
enhance
specificity
accuracy
nucleic
acid
based
on
we
have
developed
CRISPR-Cas12a-mediated
SERS
technique
identify
target
DNA,
harnessing
targeting
recognition
capability
CRISPR-Cas12a
ultra-sensitive
tags
successfully
addressing
SERS'
lack
capability.
This
system
includes
gold/nickel
foam
substrate
(Au-NFs)
reporter
(ssDNA-ROX).
The
phenomenon
colloidal
gold/silver
nano-aggregation
magnesium
ions,
which
commonly
encountered
in
CRISPR-SERS,
was
simultaneously
solved
using
AuNFs.
qualitative
quantitative
analysis
DNA
drinking
water
performed
by
monitoring
intensity
change
ROX
molecules.
results
showed
that
sensor
detected
within
30
min
limit
(LOD)
8.23
fM.
expected
become
one
alternative
rapid
high
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 19, 2024
CRISPR/Cas12a-based
biosensors
have
garnered
significant
attention
in
the
field
of
point-of-care
testing
(POCT),
yet
majority
CRISPR-based
POCT
methods
employ
fluorescent
systems
as
report
probes.
Herein,
we
a
new
CRISPR/Cas12a-enabled
multicolor
visual
biosensing
strategy
for
rapid
detection
disease
biomarkers.
The
proposed
assay
provided
vivid
color
responses
to
enhance
accuracy
detection.
In
existence
target,
