Differences in in vitro bioavailability, bioaccessibility, and antioxidant capacity depending on linkage type of luteolin 4′-O-glucosides
Food Research International,
Год журнала:
2025,
Номер
202, С. 115746 - 115746
Опубликована: Янв. 14, 2025
Язык: Английский
Synergistic Engineering of the Twin-Arginine Translocation (Tat) Pathway and Membrane Capacity Enhances Extracellular Production of Amylosucrase in Bacillus licheniformis
Microorganisms,
Год журнала:
2025,
Номер
13(6), С. 1179 - 1179
Опубликована: Май 22, 2025
Amylosucrase
(AS)
is
a
highly
versatile
enzyme
with
significant
potential
for
industrial
applications,
including
functional
food
production
and
glycosylation
of
bioactive
compounds.
However,
its
large-scale
hampered
by
low
secretion
efficiency
in
microbial
hosts.
This
study
focuses
on
engineering
the
twin-arginine
translocation
(TAT)
pathway
optimizing
membrane
resource
allocation
Bacillus
licheniformis
to
enhance
extracellular
Neisseria
polysaccharea
amylosucrase
(NpAS).
The
investigation
integrates
three
targeted
strategies:
hydrophobic
region
adjacent
TAT
signal
peptide,
modifying
translocases
via
site-directed
mutagenesis,
improving
inter-pathway
redistribution
deleting
non-essential
Sec
components.
Among
engineered
strains,
BLΔDF93S-2.0AS1
achieved
an
activity
706.10
U/L,
equating
2.01-fold
improvement
over
parental
strain.
These
results
emphasize
combining
multifaceted
strategies
optimize
heterologous
protein
systems.
Язык: Английский
Regulating the rate-limiting step of cyclodextrin glycosyltransferase glycosylation reaction for efficient production of glycosylated polyphenols
Bioresource Technology,
Год журнала:
2025,
Номер
unknown, С. 132727 - 132727
Опубликована: Май 1, 2025
Язык: Английский
Glycosylation of flavonoids by sucrose- and starch-utilizing glycoside hydrolases: A practical approach to enhance glycodiversification
Yuqing Tian,
Wei Xu,
Cuie Guang
и другие.
Critical Reviews in Food Science and Nutrition,
Год журнала:
2023,
Номер
64(21), С. 7408 - 7425
Опубликована: Март 6, 2023
Flavonoids
are
ubiquitous
and
diverse
in
plants
inseparable
from
the
human
diet.
However,
terms
of
health,
their
further
research
application
functional
food
pharmaceutical
industries
hindered
by
low
water
solubility.
Therefore,
flavonoid
glycosylation
has
recently
attracted
attention
because
it
can
modulate
physicochemical
biochemical
properties
flavonoids.
This
review
represents
a
comprehensive
overview
O-glycosylation
flavonoids
catalyzed
sucrose-
starch-utilizing
glycoside
hydrolases
(GHs).
The
characteristics
this
feasible
biosynthesis
approach
systematically
summarized,
including
catalytic
mechanism,
specificity,
reaction
conditions,
yields
enzymatic
reaction,
as
well
bioactivities
product
glycosides.
cheap
glycosyl
donor
substrates
high
undoubtedly
make
practical
modification
to
enhance
glycodiversification.
Язык: Английский
Natural Amino Acid-Bearing Carbamate Prodrugs of Daidzein Increase Water Solubility and Improve Phase II Metabolic Stability for Enhanced Oral Bioavailability
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(15), С. 8618 - 8631
Опубликована: Апрель 3, 2024
Daidzein
(DAN)
is
an
isoflavone,
and
it
often
found
in
its
natural
form
soybean
food
supplements.
DAN
has
poor
bioavailability
owing
to
extremely
low
water
solubility
first-pass
metabolism.
Herein,
we
hypothesized
that
a
bioactivatable
amino
acid-bearing
carbamate
prodrug
strategy
could
increase
the
metabolic
stability
of
DAN.
To
test
our
hypothesis,
nine
acid
prodrugs
were
designed
synthesized.
Compared
with
DAN,
optimal
(daidzein-4′-O-CO-N-isoleucine,
D-4′-I)
demonstrated
enhanced
improved
phase
II
activation
plasma.
In
addition,
unlike
passive
transport
D-4′-I
maintained
high
permeability
via
organic
anion-transporting
polypeptide
2B1
(OATP2B1)-mediated
transport.
Importantly,
increased
oral
by
15.5-fold,
reduced
gender
difference,
extended
linear
absorption
capacity
pharmacokinetics
rats.
Furthermore,
exhibited
dose-dependent
protection
against
liver
injury.
Thus,
shows
potential
increasing
improving
enhance
Язык: Английский
Efficient biotransformation of naringenin to naringenin α-glucoside, a novel α-glucosidase inhibitor, by amylosucrase from Deinococcus wulumuquiensis
Food Chemistry,
Год журнала:
2024,
Номер
448, С. 139182 - 139182
Опубликована: Март 30, 2024
Язык: Английский
Efficient and novel biosynthesis of myricetin α-triglucoside with improved solubility using amylosucrase from Deinococcus deserti
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
273, С. 133205 - 133205
Опубликована: Июнь 15, 2024
Язык: Английский
Production of highly water-soluble genistein α-diglucoside using an engineered O-α-glycoligase with enhanced transglycosylation activity and altered substrate specificity
Food Chemistry,
Год журнала:
2023,
Номер
437, С. 137898 - 137898
Опубликована: Ноя. 1, 2023
Язык: Английский
Efficient Biosynthesis of Theanderose, a Potent Prebiotic, Using Amylosucrase from Deinococcus deserti
Jeon-Uk Kang,
Yun-Sang So,
Gyungcheon Kim
и другие.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(45), С. 25197 - 25209
Опубликована: Окт. 31, 2024
The
study
aimed
to
develop
an
efficient
bioprocess
for
the
discovery
and
synthesis
of
theanderose
by
using
amylosucrase
from
Deinococcus
deserti
(DdAS).
An
unknown
trisaccharide
produced
DdAS
was
detected
high-performance
anion-exchange
chromatography-pulsed
amperometric
detection
liquid
chromatography-evaporative
light
scattering
detection,
purified
medium-pressure
chromatography,
identified
as
(α-d-glucopyranosyl-(1→6)-α-d-glucopyranosyl-(1→2)-β-d-fructofuranoside)
through
nuclear
magnetic
resonance
mass
spectrometry.
synthesized
with
a
25.4%
yield
(174.1
g/L)
2.0
M
sucrose
at
40
°C
96
h.
In
in
vitro
digestion
model,
showed
6.5%
hydrolysis
rate
over
16
Prebiotic
efficacy
tests
confirmed
that
significantly
enhanced
proliferation
selected
Bifidobacterium
strains
culturing
medium
main
carbon
source.
Subsequently,
fecal
fermentation
performed
adding
feces
20
individuals
varying
ages
assess
its
effect
on
gut
microbiota.
Theanderose
increased
relative
abundance
Bifidobacteriaceae
Prevotellaceae
while
decreasing
population
ratio
Lachnospiraceae
Ruminococcaceae.
Conclusively,
displayed
excellent
prebiotic
potential
when
judged
low
digestibility
selective
growth
beneficial
microbes
harmful
microbes.
Язык: Английский
Bioaccessibility and Bioavailability of Soybean Isoflavones: An In Vitro Study
Food Reviews International,
Год журнала:
2023,
Номер
40(8), С. 2129 - 2146
Опубликована: Сен. 7, 2023
Soybean
(Glycine
max
[L.]
Merr.),
one
of
the
top
10
crops
produced
worldwide,
is
a
significant
dietary
source
isoflavones.
Understanding
bioaccessibility
and
bioavailability
soy
isoflavones
(SIs)
important
fgor
comprehending
their
various
bioactivities
in
human
body.
In
vitro
approaches
can
provide
valuable
insights
into
SIs
across
food
matrices
step-by-step
digestive
mechanisms.
This
review
focused
on
evaluating
reports
that
employ
methods
to
study
SIs,
using
human-simulated
gastrointestinal
system
models
Transwell
with
Caco-2
cell
monolayers.
Specifically,
this
discusses
how
molecular
structures
influence
bioavailability.
The
differences
SI
aglycones
conjugated
indicate
structure
critical
factor
influencing
SIs.
provides
information
for
understanding
physiological
effects
soybean
or
SI-containing
foods.
Язык: Английский