Insight into the key bridge for infant’s nutrition and health: how to explore personalized utilization pathways from diverse human milk oligosaccharides DOI Creative Commons
Youyou Lü, Huaxi Yi, Yanchun Shao

и другие.

Food Science of Animal Products, Год журнала: 2024, Номер 2(1), С. 9240052 - 9240052

Опубликована: Март 1, 2024

Breast milk is the preferred gold standard food for infants. Human oligosaccharides (HMOs) are third most natural component in breast milk. But often insufficient, so they rely solely on substitutes. HMOs provide nutrients to beneficial gut microbiota such as Lactobacilli and Bifidobacteria, helping establish maintain a balance of microbial communities infant gut. mimic receptors pathogens, preventing them from attaching baby's intestinal cells, thereby pathogen infection. This function particularly crucial newborns How individually use important. We focused classification characteristics HMOs, their impact, intake, development/utilization mechanism health, aiming data support development. quite different (more than 200 kinds), it necessary make targeted selection, those associated with microorganisms (Bifidobacterium), which can utilize have greatest application potential. Oligosaccharide-binding protein (OBPs) an important medium ATP-binding cassette transporter channel transport; influence key OBPs Bifidobacterium recognition infants various countries has been explored, helpful accelerate establishment precise personalized powder future. The more significance results this review help consumers better choose promoting long-term health infants, especially early development immune system.

Язык: Английский

Engineered β1-3-N-Acetylglucosaminyltransferase Facilitating the One-Pot Multienzyme Synthesis of Human Milk Oligosaccharides DOI

Pei Pu,

Jie Zheng, Meng Qiao

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(50), С. 28019 - 28027

Опубликована: Дек. 6, 2024

β1-3-linked N-acetylglucosaminide is a prevalent carbohydrate motif found in oligosaccharides, polysaccharides, glycoproteins, and glycolipids. It crucial component of human milk oligosaccharides (HMOs). Neisseria meningitidis β1-3-N-acetylglucosaminyltransferase (NmLgtA) catalyzes the formation glycosidic bond has potential for use synthesizing HMOs. However, this application hindered by challenges such as low levels enzyme expression, poor stability, significant aggregation. Since there no available crystal structure NmLgtA, we used its AlphaFold 2 predicted to identify unfavorable factors. We then modified removing 17 N-terminal amino acids substituting nine specific residues. The engineered NmLgtA-Opti exhibited improved thermal increased soluble protein complete relief from aggregation, enhanced catalysis while maintaining catalytic specificity substrate promiscuity. Furthermore, maximizes utilization can be employed sequential one-pot multienzyme platform high-yield production

Язык: Английский

Процитировано

2
Selective microbial production of lacto-N-fucopentaose I in Escherichia coli using engineered α-1,2-fucosyltransferases DOI

Shun Endo,

Tomotoshi Sugita,

Sayaka Kamai

и другие.

Metabolic Engineering, Год журнала: 2023, Номер 82, С. 1 - 11

Опубликована: Дек. 24, 2023

Язык: Английский

Процитировано

5
Modular bioengineering of whole-cell catalysis for sialo-oligosaccharide production: coordinated co-expression of CMP-sialic acid synthetase and sialyltransferase DOI Creative Commons
Sabine Schelch, Manuel Eibinger,

Jasmin Zuson

и другие.

Microbial Cell Factories, Год журнала: 2023, Номер 22(1)

Опубликована: Ноя. 27, 2023

In whole-cell bio-catalysis, the biosystems engineering paradigm shifts from global reconfiguration of cellular metabolism as in fermentation to a more focused, and easily modularized, optimization comparably short cascade reactions. Human milk oligosaccharides (HMO) constitute an important field for synthetic application bio-catalysis resting or non-living cells. Here, we analyzed central catalytic module synthesis HMO-type sialo-oligosaccharides, comprised CMP-sialic acid synthetase (CSS) sialyltransferase (SiaT), with specific aim coordinated enzyme co-expression E. coli reaction flux whole cell conversions producing 3'-sialyllactose (3SL).Difference activity (CSS Neisseria meningitidis: 36 U/mg; α2,3-SiaT Pasteurella dagmatis: 5.7 U/mg) was compensated by differential protein tailored plasmid constructs, giving balance between individual activities at high level both (α2,3-SiaT: 9.4 × 102 U/g dry mass; CSS: 3.4 mass). Finally, selection guided kinetic modeling coupled CSS-SiaT reactions combination comprehensive analytical tracking multistep conversion (lactose, N-acetyl neuraminic (Neu5Ac), cytidine 5'-triphosphate; each up 100 mM). The half-life SiaT permeabilized cells (≤ 4 h) determined efficiency 3SL production 37 °C. Reaction 25 °C gave (40 ± g/L) ∼ 70% yield within 3 h, reaching mass-specific productivity g/(g avoiding intermediary CMP-Neu5Ac accumulation.Collectively, balanced CSS yields efficient (high-flux) sialylation support flexible development catalysts sialo-oligosaccharide production.

Язык: Английский

Процитировано

3
Microbial engineering for shikimate biosynthesis DOI
Qiang Ding, Chao Ye

Enzyme and Microbial Technology, Год журнала: 2023, Номер 170, С. 110306 - 110306

Опубликована: Авг. 15, 2023

Язык: Английский

Процитировано

2
Insight into the key bridge for infant’s nutrition and health: how to explore personalized utilization pathways from diverse human milk oligosaccharides DOI Creative Commons
Youyou Lü, Huaxi Yi, Yanchun Shao

и другие.

Food Science of Animal Products, Год журнала: 2024, Номер 2(1), С. 9240052 - 9240052

Опубликована: Март 1, 2024

Breast milk is the preferred gold standard food for infants. Human oligosaccharides (HMOs) are third most natural component in breast milk. But often insufficient, so they rely solely on substitutes. HMOs provide nutrients to beneficial gut microbiota such as Lactobacilli and Bifidobacteria, helping establish maintain a balance of microbial communities infant gut. mimic receptors pathogens, preventing them from attaching baby's intestinal cells, thereby pathogen infection. This function particularly crucial newborns How individually use important. We focused classification characteristics HMOs, their impact, intake, development/utilization mechanism health, aiming data support development. quite different (more than 200 kinds), it necessary make targeted selection, those associated with microorganisms (Bifidobacterium), which can utilize have greatest application potential. Oligosaccharide-binding protein (OBPs) an important medium ATP-binding cassette transporter channel transport; influence key OBPs Bifidobacterium recognition infants various countries has been explored, helpful accelerate establishment precise personalized powder future. The more significance results this review help consumers better choose promoting long-term health infants, especially early development immune system.

Язык: Английский

Процитировано

0