Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown
Опубликована: Дек. 19, 2024
Difucosyllactose (DFL), an important kind of fucosylated human milk oligosaccharides (HMOs), has garnered considerable attention due to its excellent physiological activities in infants. Previously, we achieved de novo biosynthesis DFL; however, substantial residual intermediates fucosyllactoses (FL) were detected. In this study, DFL was optimized, and FL reduced by regulating metabolic pathways. Different plasmid combinations used regulate gene expression, achieving optimal flux balance between 2′-FL DFL. The expression level key enzyme α-1,3-fucosyltransferase (α-1,3-FT, FucTa) then enhanced increasing copy number integrating fucTa into the chromosome. Exocytosis deleting sugar efflux transporter setA gene, thereby minimizing FL. Finally, strain BSF41 produced 55.3 g/L with only 2.59 a 5 L fermentor, representing highest reported titer date. This study provides foundation for advancing HMOs.
Язык: Английский