Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
73(2), С. 1694 - 1702
Опубликована: Дек. 30, 2024
This
study
has
developed
a
pressure
sensor
array
based
on
four
functionalized
DNA-nanoenzymes
with
catalase-like
activity
for
multiple
detections
of
foodborne
pathogens
through
portable
manometer.
Benefiting
from
functionalization
4-mercaptophenylboronic
acid
and
β-mercaptoethylamine,
the
diversity
nonspecific
interactions
between
each
nine
bacteria
leads
to
differences
in
response
patterns
by
catalyzing
H2O2
generate
exclusive
"fingerprints".
As
effective
statistical
tools
processing
multivariate
data,
principal
component
analysis
hierarchical
clustering
are
employed
identify
analyzing
patterns.
Furthermore,
as-prepared
can
discriminate
different
mixtures
achieve
quantitative
detection,
an
average
detection
limit
102
104
CFU
mL–1
Gram-positive
Gram-negative
bacteria,
respectively,
demonstrating
its
desirable
practicality
satisfactory
accuracy
real
sample
detection.
expands
insights
into
analyses
food
safety
monitoring.
ACS Sensors,
Год журнала:
2024,
Номер
9(6), С. 2815 - 2825
Опубликована: Май 17, 2024
Bacterial
toxins
emerge
as
the
primary
triggers
of
foodborne
illnesses,
posing
a
significant
threat
to
human
health.
To
ensure
food
safety,
it
is
imperative
implement
point-of-care
testing
methods.
Lateral
flow
biosensors
(LFBs)
based
on
gold
nanoparticles
(GNPs)
have
been
commonly
used
for
rapid
detection,
but
their
applicationis
limited
by
low
sensitivity.
Based
localized
surface
plasmon
resonance
and
photothermal
effect
dual
nanoparticle
conjugates
(DGNPs),
we
developed
smartphone-integrated
LFB
(PLFB)
with
double-enhanced
colorimetric
Through
numerical
simulations,
verified
that
DGNPs
significantly
enhanced
performance
compared
single
15
nm
GNPs
(SGNPs),
applied
in
PLFB
detection
staphylococcus
enterotoxin
A
(SEA).
The
limits
DGNPs-based
SEA
were
0.091
0.0038
ng
mL–1,
respectively.
Compared
SGNPs-based
LFB,
sensitivity
was
increased
10.7
times,
further
improved
255.3
times.
Moreover,
exhibits
robust
reproducibility
exceptional
specificity
applicable
detecting
milk
samples.
This
allows
users
detect
simply,
conveniently,
quickly
has
huge
application
potential
field
safety.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(22), С. 9270 - 9277
Опубликована: Май 21, 2024
Developing
a
specific,
sensitive,
rapid,
and
on-site
method
for
detecting
pathogenic
bacteria
in
food
samples
is
critical
to
ensuring
public
safety.
This
article
demonstrates
CRISPR/Cas13a
system
chemiluminescence
resonance
energy
transfer
(CRET)
(CRISPR/Cas
13a-assisted
CRET)-based
strategy
sensitive
detection
of
real
samples.
Once
the
hybrid
double
strand
aptamerS.
aureus-cRNA
recognizes
target
model
Staphylococcus
aureus
(S.
aureus),
released
cRNA
would
bind
with
CRISPR/Cas
13a
form
complex
cRNA-CRISPR/Cas
13a,
which
could
cleave
RNA
molecule
probe
horseradish
peroxidase
(HRP)
modified-gold
nanoparticles
(AuNPs)
linked
by
(AuNPs-RNA-HRP),
resulting
an
enhanced
signal
due
CRET
"OFF"
phenomenon
after
introducing
substrate
luminol.
The
successfully
detected
S.
drinking
water
milk
limits
20
30
cfu/mL,
respectively,
within
recovery
90.07–105.50%.
Furthermore,
integrating
immunochromatographic
test
strip
(ICTS),
achieved
as
low
102
cfu/mL
via
smartphone,
about
10
times
lower
than
that
previously
reported
AuNPs-based
colorimetric
ICTS,
demonstrating
convenient
Trends in Food Science & Technology,
Год журнала:
2024,
Номер
147, С. 104472 - 104472
Опубликована: Март 28, 2024
Analytical
methods
play
a
crucial
role
in
verifying
the
authenticity
of
food
products
to
ensure
consumer
safety,
prevent
fraud,
and
maintain
regulatory
compliance
global
industry.
However,
conventional
such
as
DNA
testing,
spectroscopy,
chromatography,
heavily
reliant
on
laboratory
settings,
have
posed
significant
challenges
authentication
detection.
The
recent
integration
nanozymes
into
lateral
flow
assays
(LFAs)
offers
robust
solution
for
identifying
quantifying
contaminations
adulterations
with
improved
sensitivity,
efficiency,
versatility.
In
this
review,
advancements
nanozyme-enhanced
LFAs
tailored
were
examined.
A
thorough
discussion
is
first
presented
by
exploring
diverse
catalytic
activities.
review
further
highlights
nanozyme-incorporated
detection
across
various
applications
authentication.
has
significantly
heightened
precision
efficiency
Concurrently,
advanced
contribute
developing
multi-functional,
portable,
point-of-care
(POC)
devices.
synergy
between
nanozyme
enhancement
results
superior
compared
traditional
methods.
While
successful
are
highlighted,
article
also
addresses
provides
forward-looking
perspective
future
ensuring
real-world
industrial
POC
applications.
Food Chemistry X,
Год журнала:
2023,
Номер
20, С. 100990 - 100990
Опубликована: Ноя. 11, 2023
Photothermal
immunochromatographic
sensor
is
an
emerging
detection
technology,
and
it
important
to
develop
new
sensing
probes
with
excellent
photothermal
performance
improve
its
performance.
In
the
present
study,
a
novel
probe
based
on
violet
phosphorus
nanosheets
satisfactory
conversion
efficiency
(31.1
%)
was
reported
for
first
time.
A
using
above
applied
visual
of
diethylstilbestrol.
The
diethylstilbestrol
concentration
inversely
proportional
signal
showed
good
linear
correlation
in
range
0.75
∼
50
μg·L-1.
After
optimizing,
limits
were
6
μg·L-1
0.56
μg·L-1,
respectively.
recovery
rates
tap
water,
milk
pork
samples
ranged
from
82.2
%
115.2
%,
coefficient
variation
(CV)
ranging
2.0
10.8
%.
This
work
not
only
structured
type
probe,
but
also
expanded
application
phosphorus.
